ATX induces alterations on autophagy in neuron-like cells. (A) Representative western blots and (B) quantification analysis of LC3-II levels. β-actin was used as a loading control. (C) Representative confocal images showed the LC3B-GFP puncta formation with different concentrations of ATX. Data are mean ± SEM, and values are from three independent experiments. *P < 0.05 compared to the control group. Scale bar = 10 μm. For clarity of the results, the representative Western blots and confocal images are cropped, for raw data see Supplementary Information.