Aspergillus fumigatus establishes infection in zebrafish by germination of phagocytized conidia, while Aspergillus niger relies on extracellular germination

Among opportunistically pathogenic filamentous fungi of the Aspergillus genus, Aspergillus fumigatus stands out as a drastically more prevalent cause of infection than others. Utilizing the zebrafish embryo model, we applied a combination of non-invasive real-time imaging and genetic approaches to compare the infectious development of A. fumigatus with that of the less pathogenic A. niger. We found that both species evoke similar immune cell migratory responses, but A. fumigatus is more efficiently phagocytized than A. niger. Though efficiently phagocytized, A. fumigatus conidia retains the ability to germinate and form hyphae from inside macrophages leading to serious infection even at relatively low infectious burdens. By contrast, A. niger appears to rely on extracellular germination, and rapid hyphal growth to establish infection. Despite these differences in the mechanism of infection between the species, galactofuranose mutant strains of both A. fumigatus and A. niger display attenuated pathogenesis. However, deficiency in this cell wall component has a stronger impact on A. niger, which is dependent on rapid extracellular hyphal growth. In conclusion, we uncover differences in the interaction of the two fungal species with innate immune cells, noticeable from very early stages of infection, which drive a divergence in their route to establishing infections.

The Aspergillus genus comprises over 200 species of filamentous fungi. Though normally harmless, several Aspergillus species have been described as opportunistic pathogens, capable of causing invasive aspergillosis, a severe condition characterized by the germination of spores, growth and penetration of fungal hyphae into host tissues. A. fumigatus is the dominating causative agent in invasive aspergillosis with four species (A. niger, A. flavus, A. tereus and A. nidulans) accounting for almost all remaining incidences 1 . Several different features of A. fumigatus physiology have been proposed to account for its higher virulence, including the size of the conidia, their ubiquitous abundance and the hydrophobicity of the A. fumigatus cell wall making the spreading of their conidia highly efficient 2,3 . In addition to these features, however, it seems clear that different pathogenic species of Aspergillus may interact with components of the host innate immune system in fundamentally different ways which may be another important factor in the increased pathogenicity of a. A. fumigatus compared to other Aspergillus species 4 . For this reason, thorough characterization of the interactions between host and pathogen, as well as the identification of common factors that may influence virulence in similar ways across species, is of interest as potential targets for therapeutic interventions.
The fungal cell wall is essential to the interactions between the fungus and the host immune system. The fungal cell wall is a protective structure consisting of a complex mixture of various polysaccharides and glycoproteins. The outer layer of the cell wall is enriched in glycoproteins which are retained in the cell wall by ionic interactions or by covalent linkage via remnants of GPI-anchors to the glycan/chitin part of the cell wall. The inner layer of the cell wall consists of mainly of β-1,3-glucan and chitin. These structural components are cross-linked to each other and form an important scaffold to which other cell wall components (α-glucan, galactomannan and glycoproteins) can bind 5,6 . Galactofuranose (Galf) containing glycostructures (mainly galactomannan and galactomannoproteins) are common components of Aspergillus cell walls 7 and Galf biosynthesis is important to maintain cell wall integrity. Mutations or deletions of genes required for Galf-biosynthesis lead to higher sensitivity towards antifungal drugs [8][9][10] . UDP-galactopyranose mutase is a crucial enzyme in the biosynthesis of Galf-containing glycoconjugates. The enzyme is required to convert UDP-galactopyranose into UDP-galactofuranose which is used as a nucleotide sugar donor for the transfer of Galf via Golgi localized Galf-transferases 11 . The gene encoding UDP-galactopyranose mutase (named ugmA in A. niger and A. nidulans, and glfA in A. fumigatus) has been shown to be required for Galf biosynthesis 8,9,12,13 .
From a pharmaceutical perspective, Galf-containing glycostructures are interesting for two reasons. First, the presence or absence of Galf in A. fumigatus has been shown to influence virulence and resistance to antifungal drugs in immunosuppressed mice, making it an interesting target either on its own or in combination with other antifungal agents 9 . Second, while the six-membered pyranosyl form of the hexose monosaccharide galactose is found in many vertebrate species as part of complex glycoconjugates of proteins and lipids 14 , the five-membered furanosyl form is not found in higher vertebrates 7 , thus making it a candidate for selective pharmaceutical intervention. Immunogenic properties of Galf have been shown in vitro 15 , but the importance of Galf as a pathogen-associated molecular pattern (PAMP) has been contested in vivo 16 . Further studies into the effects of mutations of genes involved in the biosynthesis of Galf in relation to infectious behavior are required to establish the role of Galf and derived glycosides in pathogenic fungal infection.
The zebrafish embryo is an attractive model for in vivo studies of Aspergillus infection. The optical transparency of the embryo makes it very tractable for fluorescence based live in vivo studies of the development of fungal morphology and the interactions of the fungal pathogen with innate immune cells. While the embryo possesses a functional innate immune system with macrophages and neutrophils capable of phagocytizing microbes as early as 30 hours post fertilization 17,18 , the first mature lymphocytes do not appear until 3 weeks post fertilization with the progressing development of the pronephronic tissues from which these cells primarily originate [19][20][21] . This temporal gap between the emergence of components of innate and acquired immunity allows for studying opportunistic infections without the need for immunosuppression of the host. The feasibility of this concept has recently been demonstrated by infections with A. fumigatus 22 , Mucor circinelloides 23 and Cryptococcus neoformans 23,24 , and as the hindbrain ventricle is devoid of leukocytes at the early stages of embryonic development 17 it has been widely utilized to study leukocyte migratory patterns.
Here we utilize the zebrafish embryonic hindbrain injection model to characterize the disease progression resulting from A. fumigatus and A. niger conidia injections in terms of immune cell interactions and embryonic survival, and compare the results to those of the Galf-deficient mutants. We find that A. niger and A. fumigatus elicit similar immune responses, but interact with innate immune cell populations differently, causing the two Aspergillus species to follow diverging paths of pathogenic development. Whereas A. niger appears dependent on rapid growth to overwhelm the innate immune response, A. fumigatus is able to germinate and grow after the conidia have been phagocytized by macrophages. Yet, in spite of the clear differences in infectious development, the Galf-deficient strains of both species are significantly attenuated in pathogenicity. The impeded virulence of the Galf-deficient strains appears to correlate most strongly with decreased hyphal growth rate. Therefore, in agreement with the notion that A. niger proliferation is more dependent on speed of growth, we find the effect of Galf deficiency to be more drastic in this species. These results demonstrate the value of the zebrafish embryo model for comparative in vivo studies of Aspergillus infection and evaluating the impact of cell wall components.

Results
Aspergillus niger and Aspergillus fumigatus exhibit differences in infectious behavior that are related to their interactions with host innate immune cells. To investigate the pathogenic behavior of A. niger and A. fumigatus we applied the approach of Knox et al. 22 for hindbrain ventricle injection of fungal conidia in zebrafish embryos. Forming a cavity lined with epithelium, the hindbrain ventricle in zebrafish embryos is commonly used to mimic the conditions of alveolar infections [24][25][26] . This is an infection route that we and others have successfully used to study macrophage and neutrophil responses to bacterial infections [25][26][27] . Using this approach, we investigated the survival of control embryos versus pu.1 morpholino injected embryos after infection with A. niger and A. fumigatus. Morpholino knockdown of pu.1 causes a blockage of myelopoiesis, the process by which the macrophages and neutrophils develop 28 , thus rendering the pu.1 morphant embryo deficient in cellular innate immune responses for the duration of the experiment. Our observations revealed that A. niger and A. fumigatus both exhibited robust pathogenicity over 96 hours in survival rate assays at high infectious burdens of 150 conidia (Fig. 1A), and in both cases the innate immune system appeared to confer significant protection, with a hazard ratio of approximately 6 between controls and pu.1 morphants for A. niger and over 14 for A. fumigatus. However, though overall mortality rates to these high infectious burdens were similar at the termination of the experiment, 96 hours post infection (HPI), there was a marked difference in the temporal development of survival rate assays between the two Aspergillus species. While the profile of A. niger was characterized by significant mortality at 24 HPI, the sharpest drop in survival in the case of A. fumigatus occurred at later time points. This indicates that the development of infection by the two different fungal pathogens is different, causing mortality to occur later in A. fumigatus infection (Fig. 1A,B). Decreasing the fungal challenge dose revealed that the mortality of A. niger was more dependent on high infectious burdens than A. fumigatus, as A. niger caused no mortality with a challenge dose of 50 conidia, while A. fumigatus still induced significant mortality at this challenge dose (Fig. 1C). This is consistent with the general view that A. fumigatus is more pathogenic than A. niger. The protective effect of the host immune system was once again demonstrated as both A. niger and A. fumigatus induced nearly complete mortality in immunodeficient pu.1 morphants. The protective effect, as quantified by the hazard ratios between control and pu.1 morphants confirm this observation; while pu.1 morphants are 24 times www.nature.com/scientificreports www.nature.com/scientificreports/ more likely to die from dose of 50 conidia of A. fumigatus over 96 hours than control embryos, that ratio is nearly 42 for A. niger. Thus, it appears that the interactions of the injected fungal conidia with the innate immune system shapes the outcome of the infection, and that the innate immune system confers a greater protection against a low challenge dose of A. niger than against A. fumigatus. As we wished to investigate the germination and hyphal growth phases of infectious development we continued with the higher challenge doses of 150 conidia in the subsequent experiments. In summary, these survival rate analyses indicated that A. niger and A. fumigatus both exhibit robust pathogenicity when high infectious burdens of 150 conidia are applied, but that there is a temporal difference in the infectious development, whereby A. niger induces robust mortality within the first 24 hours, while A. fumigatus takes longer to deliver a lethal outcome. The innate immune system confers significant protection against both species of Aspergillus, but while the innate immune system is more protective against A. fumigatus than A. niger at high challenge doses, this is reversed at lower challenge doses. All these observations led us to conclude that the two Aspergillus species develop infection through different routes, and that the differences are likely related to interactions with the innate host immune system.
Both species induce similar leukocyte migratory responses but Aspergillus fumigatus is more efficiently phagocytized than Aspergillus niger. We wished to investigate whether the apparent difference in the infectious progression between A. niger and A. fumigatus was caused by differences in their interactions with the different subsets of innate immune cells. To approach this question, we characterized the nature of these interactions in the hours immediately subsequent to infection. To this end, we utilized a dual reporter zebrafish line with labelled neutrophils 29 and macrophages 30 in green and red, respectively. As the mCherry signal labelling the macrophages in this line is membrane bound, we were able to use morphology and brightness of fluorescent signal to distinguish between macrophages and fungi ( Fig. 2B,C). Examining confocal slices carefully we were able to establish a difference in the efficiency of phagocytosis between the two Aspergillus species, as early as 7 hours post infection (HPI), before germination of the conidia. We found that A. fumigatus was significantly more efficiently phagocytized than A. niger. Overall, on average 7,3% of the conidia of A. niger were free and unphagocytized, at 7 HPI, against just 1,2% unphagocytized A. fumigatus conidia (Fig. 2D). Using the same approach we investigated the migratory behavior of each leukocyte population. Again we relied on morphology and brightness of fluorescent signal to distinguish between macrophages and fungi (Fig. 3). To investigate the development over time and assess the importance of fungal lifecycle dynamics we included live and heat killed conidia, at 7 and 24 HPI. The quantification results indicated quite similar leukocyte migratory behavior directed against both species of Aspergillus. Heat killed conidia of either species elicited a significant macrophage migratory response indicating that the initial macrophage response is likely to be independent of the maturation and swelling of the conidia prior to germination (Fig. 3). However, while the leukocyte presence in response to live conidia of both species were significantly elevated at 24 HPI the leukocyte numbers in subjects injected with heat killed conidia of both species had reverted to the level of control injected subjects. Together with the survival rate observations this indicates that the efficient phagocytosis of injected A. fumigatus is not sufficient to resolve the infection, and that significant neutrophil influx occurs later than is the case of A. niger.
Aspergillus niger often germinate and grows hyphae extracellularly while Aspergillus fumigatus conidia persist, germinate, and grow from inside macrophages. Following the development in confocal time lapse experiments (between 4 and 24 HPI), the initial differences in infectious behavior remained evident and were further perpetuated as the conidia germinated and hyphal growth commenced. In A. niger infection, in every instance when germination was observed, it occurred from non-phagocytized conidia with germination and hyphal growth commencing as early as 9-12 HPI (more than 100 observations of germination events from embryos injected in 3 independent experiments) (see Supplementary Movies SM1, SM14 and SM15). The outcome, in terms of larval survival appeared to be dependent on the success of immune cells in the ensuing, highly inflammatory response, to attenuate the growth of the hyphae (Fig. 4B,C and Supplementary Movies SM1, SM2, SM14 and SM15). The more rapid development observed in A. niger infection appears in good agreement with our initial observations that A. niger caused mortality more rapidly than A. fumigatus (Fig. 1).
Since A. fumigatus conidia were efficiently phagocytized by macrophages in the hours immediately after injection, it stands to reason that the development of infection and pathogenicity would be different from that of A. niger. Indeed, in the cases analyzed, A. fumigatus germination and hyphal growth occurred later than in the case of A. niger, (compare Fig. 4B,D) commencing around 24-48 HPI (see Supplementary Movie SM5). Confocal microscopy revealed that, at least in some cases, A. fumigatus hyphal growth occurred from conidia which had been phagocytized by macrophages (Fig. 4E). This was an event we had not observed in A. niger infection, though we cannot rule out that it may happen occasionally. With the aim to capture the event of germination and hyphal growth from macrophages after phagocytosis in a time lapse movie, and to further clarify the role of neutrophils and macrophages in the defense against A. fumigatus infection, we performed another experiment in which we commenced the time-lapse acquisition at 24 HPI, using the dual fluorescent reporter line to follow macrophages and neutrophils. Acquisition continued over a time period of 16 hours (Supplementary Movie SM5). In our interpretation this captures several macrophages, heavily laden with A. fumigatus conidia, which germinate and grow hyphae from within the macrophages after phagocytosis. Taken together, these observations point to a fundamentally different mode of pathological development in the two Aspergillus species. Whereas A. niger appears dependent on evading the innate immune cells long enough to germinate and grow hyphae, A. fumigatus appears to be able to withstand phagocytosis and to grow hyphae, which will eventually burst through the phagocyte. This is in good agreement with the results from the survival rate experiments, indicating that A. niger causes very little mortality unless it is delivered at a dose that can overwhelm the immune response.   (Fig. 5). The result clearly shows that Galf-deficient strains of both Aspergillus species exhibit attenuated pathogenicity. As was the case with the WT strains, protection from infection and pathogenicity of the Galf-deficient strains in both species was clearly dependent on a functioning innate immune system, as immunocompromised pu.1 morphants, exhibited drastically increased mortality, compared to the immunocompetent controls (Fig. 5). Following the immune cell migratory responses by time lapse confocal microscopy the Galf mutants appear to elicit similar innate immune responses to their respective WT strains (Supplementary Movies SM6-9). Leukocyte quantification revealed similar initial inflammatory responses in terms of leukocyte migration in response to the mutants both at 7 and 24 HPI compared to their respective parental strains (Supplemental Fig. 1). Thus, it does not appear that the attenuated pathogenicity of the Galf synthesis mutants is directly related to drastically different interactions with immune cells.

Galactofuranose-deficient mutants of Aspergillus niger and
Decreased virulence of galactofuranose synthesis mutants correlates with slower hyphal growth. While investigating the pathogenicity of the mutant fungal strains it was interesting to note that while the Galf-deficient mutant strains of both Aspergillus species, like WT strains, effected nearly complete mortality in pu.1 morphants, they did so slightly more slowly than the WT strains at the same infectious burdens (Fig. 5A,B). Though the survival of embryos to the Galf deficient fungal strains was clearly dependent on cells of the innate immune system, the slight temporal delay of mortality of these fungal strains even in the absence of a functional innate immune system, indicates that elements inherent to the fungal physiology and growth are contributing to their loss of virulence. Several explanations could be offered to account for this, but most likely the attenuated pathogenicity of the Galf-deficient Aspergillus strains might be caused by a decreased germination rate or hyphal growth rate. In vitro growth rate assessment did not reveal any major attenuation of growth rate of the Galf deficient strains (Supplemental Fig. 2). Aiming to investigate this in the most appropriate environment, i.e. in an infected subject but without the growth-attenuating effects of immune cells, we analyzed confocal time-lapse videos which were acquired in parallel, to follow the germination and growth of WT and Galf-deficient mutant conidia of A. niger and A. fumigatus, in pu.1 morphant embryos. For each species and genotype we analyzed the time of germination based on the first observed germination events in pu.1 morphant embryos captured by confocal time-lapse microscopy (Fig. 6A,B). The results of these experiments revealed no significant difference in the germination time between the galf deficient mutant and parental strains of either species. With an average germination time of approximately 14 hours A. fumigatus appeared to take longer to germinate in the host environment compared to A. niger which exhibited an average germination time of 8 hours. Analyzing the increase in hyphal length between frames in the confocal time lapse microscopy series we could quantify an approximate rate of hyphal growth. The galf deficient mutant strains of both species exhibited drastically decreased hyphal growth rates compared to WT (Fig. 6C and Supplementary Movies SM10-13). We propose this attenuated hyphal growth rate of the Galf-deficient mutant strains as the most probable cause of their decreased pathogenicity.

Discussion
A. fumigatus is well known for causing more serious infections in human patients than other fungi belonging to the Aspergillus genus, but the mechanisms underlying this higher pathogenicity are poorly understood. Here we used a well-established zebrafish hindbrain infection model to study differences in virulence between A. fumigatus and A. niger, focusing on the interaction of these fungi with the host innate immune cells, macrophages and neutrophils. The model is suitable for simple yet detailed non-invasive in vivo analysis of infectious progression based on the morphological alterations of the Aspergillus early lifecycle in dynamic interactions with innate immune cells. Performing parallel experiments with A. fumigatus and A. niger allowed us to compare and contrast these two species in terms of their infectious behavior and point out fundamental differences between the two species of Aspergillus. This led us to propose an explanation for the drastically higher incidence of infection caused by A. fumigatus compared to other species of Aspergillus.
We have gathered our observations in one comprehensive model of the development of infection of the two species of Aspergillus (Fig. 7). It is plausible that the increased incidence of infection caused by A. fumigatus is, at least partially, related to the ability of this species to withstand, germinate and grow hyphae after phagocytosis by macrophages. This behavior we have not observed for A. niger. Infection by A niger would therefore be expected to occur only in the cases when conidia are allowed to germinate and grow hyphae unchallenged by the immune cells. This notion is supported by the observation that the rate of A. niger-induced mortality drops rapidly to near zero when the infectious burden is reduced to 50 conidia, while A. fumigatus maintains significant mortality at this reduced challenge dose (Fig. 1C). Thus it would appear that A. niger primarily causes progressive infection and mortality when it is applied in a sufficiently high dose to overwhelm the initial immune response, whereas A. fumigatus is able to cause a fatal infection in spite of an adequate initial macrophage response. Importantly, the lower infectious dosages where A. niger was entirely contained are likely to be closer to those a human might encounter. The primary objective of our experiments applying higher dosages, is to force an infection to occur even with the less pathogenic A. niger which allows us to use microscopy based approaches to contrast the be seen protruding from the cluster of several conidia (arrowheads). In the central panel, showing the green channel the macrophage can be seen. In the right panel, overlaying the green fluorescence channel it was clear that the cluster of conidia had been phagocytized and were contained within a macrophage. One growing hyphae could be seen to stretch the membrane of the macrophage (arrowhead). (B) White box insert in 10 HPI image represents the magnified area in image panel (C). (2019) 9:12791 | https://doi.org/10.1038/s41598-019-49284-w www.nature.com/scientificreports www.nature.com/scientificreports/ infectious route taken, and outline aspects of A. fumigatus infectious development that are likely to be critical to its enhanced pathogenicity.
Our observations regarding immune cell interactions of A. niger are difficult to discuss in a broader context as, to the best of our knowledge, no similar in vivo studies have explored A. niger infectious behavior of interactions with innate immune cells. A. fumigatus, as the more severe pathogen, has received substantial scientific attention. Our observations regarding the pathogenicity of A. fumigatus our observations indicate a more severe, and faster development of disease and mortality, than was reported in the study of Knox and coworkers 22 and as such are more in line with those reported by Herbst and coworkers 33 . The causes of these inter-lab differences are not clear but may relate to the genetic background of the WT zebrafish lines used. Whatever the underlying cause may be, one advantage of the higher observed speed of infectious development in A. fumigatus in our studies compared to the Knox paper 22 is that we can more easily capture some of the pivotal events in the progression of infection. This has allowed us to definitively show that A. fumigatus can germinate and grow from inside macrophages after phagocytosis in vivo (Fig. 4E), an event which the authors of the Knox paper remarked that they had been unable to capture. The notion that the progress of A. fumigatus infection will in some cases be initiated from within phagocytizing macrophages has been inferred from in vitro studies 4 , but to our knowledge this is the first time germination and hyphal growth from within phagocytizing macrophages has been demonstrated directly in vivo.
In the light of the observed differences between the two Aspergillus species of this study, as well as in vitro comparative studies comparing A. fumigatus to A. terreus 4 , it seems that the infectious behavior of Aspergillus may in many cases be fundamentally different across species. Therefore, it is very interesting to note that deficiency in the synthesis of the same cell wall component, galactofuranose (Galf), affects both species in a similar manner, decelerating hyphal growth and significantly attenuating pathogenicity. We believe the Galf synthesis pathway deserves further attention as a potential candidate for broad ranging Aspergillus medical intervention. The zebrafish hindbrain infection model, with its demonstrated applicability for investigations of immune cell infiltration behavior as well as a means to study the behavior of the fungal infectant in vivo, will be a valuable addition to further research efforts to resolve the relative importance of different cell wall components to overall infectious behavior of Aspergillus species. The demonstration of the A. fumigatus infectious route through the macrophage has now been observed by several independent studies apart from ours 22,33,34 . Further investigations into the relevant intracellular fungal derived signaling as well as relief strategies such as the lateral macrophage to macrophage transfer of conidia 34 35 , Tg(mpeg1:mCherryF ump2 ) 30 and Tg(mpx:GFP i114 ) 29 zebrafish lines, adults and embryos, were handled in compliance with the local animal welfare regulations and maintained according to standard protocols (zfin.org). Breeding of zebrafish adults was approved by the local animal welfare committee (DEC) of the University of the Leiden, under license number 10612 and in compliance with international guidelines specified by the EU Animal Protective Directive 2010/63/EU. All studies in this work was performed on embryos/larvae before the free feeding stage, no adult   niger. For the cell wall mutants 1 M sorbitol was added to CM. After 72 hours of growth, spores were harvested with 10 ml of PZ (0.9% (w/v) NaCl) and filtered through 2 layers of sterile Mira cloth. Spore inoculum concentration was measured by automated cell counter (Bio-Rad TC20) and adjusted to final concentration of 1.5 × 10 8 spores/ml in 2% (w/v) polyvinylpyrrolidone (PVP) in phosphate-buffered saline (PBS). The obtained spore suspensions were used within 4 weeks.
Fluorescent labeled A. niger and A. fumigatus strains were constructed by co-transformation of plasmid pgpdA-mCherry with plasmid pAN7.1 38 . Hygromycin resistant transformants were purified twice and analyzed by fluorescence microscopy to identify transformants in which the FP construct was integrated into the genome. A. niger elicits a robust migratory response from macrophages and neutrophils in the initial stages of infection. This leads to a strong innate immune cell presence before the conidia germinate, around 7-10 hours post infection (HPI). Despite the strong immune cell migration, phagocytosis is in many cases incomplete and extracellular conidia progress from dormancy to germination and hyphal growth, often between 10 and 16 HPI. Embryonic survival appears dependent on the immune system's ability to curb the germination and hyphal growth, and as such the success of A. niger to cause infection is very dependent on the magnitude of the infectious burden, and very sensitive to mutations that impede hyphal growth rates such as the ΔugmA. A. fumigatus, depicted in the lower half of the figure, causes a very similar initial immune cell migratory response. Conidia are very efficiently phagocytized, leading to the formation of large discrete clusters of phagocytized, but viable conidia inside macrophages. This development takes place within the initial hours after infection and is most often complete by 7 HPI. At later stages, starting from approximately 24 HPI, germinating conidia start to grow hyphae from within phagocytosing macrophages, while other macrophages can be observed to swarm around the growing hyphae, but seemingly unable to stop the growth.