Table 2 Primer sequences employed in the study.

From: Hepatitis C virus (HCV) genotype 1b displays higher genetic variability of hypervariable region 1 (HVR1) than genotype 3

  genotype 1b HVR1 amplification Positions of HCV genome genotype 3 HVR1 amplification Positions of HCV genome
First round PCR Forward:
5′-GGTGCTCACTGGGGAGTCCT-3′
1389–1408 Forward:
5′-ATGGCATGGGATATGAT-3′
1291–1307
Reverse:
5′-CATTGCAGTTCAGGGCCGTGCTA-3′
1632–1610 Reverse:
5′-AAGGCCGTCCTGTTGA-3′
1619–1604
Second round PCR Forward:
5′- TCCATGGTGGGGAACTGGGC-3′
1428–1447 Forward:
5′-GGCAACTGGGCCAAGGTCGC-3′
1437–1456
Reverse:
5′-TGCCAACTGCCATTGGTGTT-3′
1603–1584 Reverse:
5′-ATGTGCCACGAGCCATTGGT-3′
1606–1587