Transcriptional Analysis of Mitochondrial Complexes (n = 8). (a–e) ddPCR gene transcript counts for NADH:ubiquinone oxidoreductase subunit A1 (Ndufa1), Succinate dehydrogenase complex flavoprotein subunit A (Sdha), BCS1 homolog, ubiquinol-cytochrome c reductase complex chaperone (Bcs1l), Cytochrome c oxidase subunit 4i1 (Cox4i1), and ATP synthase 6, mitochondrial (MT-ATP6). *p < 0.0125 main effect for AGE vs. AGE-0 μg/ml, mixed effects regression. †p < 0.05 vs. HG AGE-0 μg/ml, paired t-test. NG:HG p < 0.0125 indicates main effect for glucose condition, mixed effects regression. Data presented as mean ± standard error. ■ Normal Glucose (NG), High Glucose (HG).