Expression of PCNA in binucleate T. gondii-infected BUVEC. T. gondii-infected BUVEC and control cells were stained for DNA via DAPI (blue) and for Proliferating cell nuclear antigen (PCNA, green) and analyzed via confocal microscopy. The nuclei of binucleate cells are indicated by white arrows. To analyze the nuclear PCNA distribution, we measured the intensity of PCNA-related signals by drawing a longitudinal line over the nuclei and plotting these data as a graph showing intensity value vs distance (in µm). (a) = left and (b) = right nucleus of a binucleate cell. Scale bar represents 5 µm.