Figure 5 | Scientific Reports

Figure 5

From: Toxoplasma gondii-induced host cellular cell cycle dysregulation is linked to chromosome missegregation and cytokinesis failure in primary endothelial host cells

Figure 5

Mitotic spindle and centromer formation in T. gondii-infected BUVEC. (A) T. gondii-infected BUVEC and control cells were stained for chromosomes by DAPI (blue), for mitotic spindles by α-Tubulin (red) and for centromeres by γ-Tubulin (green) and analyzed via confocal microscopy. Additionally, the intensity of centromer-related signals was assessed and plotted as a graph showing intensity value vs distance (in µm). Scale bar represents 5 µm. (B) The number of centrosomes in mitotic cells, in T.gondii-infected BUVEC and control cells, were counted, and graph as a percentage of the total mitotic cells in the monolayer. Bars represent the median ± SEM. Bars represent the median ± SEM. Nonparametric one-way ANOVA (Kruskal-Wallis post-test), **p ≤ 0.001.

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