Distribution of cell cycle phases in T. gondii-infected BUVEC and control cells and expression/phosphorylation of cyclin B1 in infected cells. (A) BUVEC were infected with T. gondii tachyzoites and examined for DNA content one day after infection applying FACS analyses. Therefore, the total amount of cells with one (G-phase) or two copies (G2-phase) of the genome was plotted as a percentage of the total cells vs DNA amount. (B) Analysis of T. gondii-infected cells vs non-infected cells originating from the same (infected) cell layer was done using an specific antibody against T. gondii tachyzoites for splitting both population. (C,D) Analysis of cyclin B1 expression and phosphorylation in T. gondii-infected BUVEC. Six biological replicates of BUVEC were analyzed by Western blotting (C) for the expression of cyclin B1 and its phosphorylated form (S126). For control, pure T. gondii tachyzoites were also analyzed. The density of the protein signals was quantified and graphed as ratio relative to vinculin as housekeeping protein (D). Bars represent the median ± SEM. Nonparametric one-way ANOVA, ***p ≤ 0.001, ***p < 0.0001.