Figure 7 | Scientific Reports

Figure 7

From: Identification of pathways that regulate circadian rhythms using a larval zebrafish small molecule screen

Figure 7

Drugs that affect Tg(per3:luc) luminescence circadian amplitude do not necessarily similarly affect locomotor activity circadian amplitude. Anti-inflammatory drug cyclosporin (A) and NF-κB inhibitor Ro 106-9920 (D) increase and decrease larval per3:luc luminescence amplitude, respectively. However, both compounds increase locomotor activity circadian amplitude in LD (B,E) and in entrained animals shifted to DD (C,F). Number of animals: (B) DMSO n = 36, cyclosporin n = 39. (C) DMSO n = 45, cyclosporin n = 34. (E) DMSO n = 45, Ro 106-9920 n = 37. (F) DMSO n = 45, Ro 106-9920 n = 40. *P < 0.05, **P < 0.01, ***P < 0.001 by ANOVA with Tukey’s test. Luminescence data shown in (A) is the same as that shown in Fig. 4A.

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