Effect of BDL in the liver and the intestine. (A) Serum liver damage markers ALT, AP and Bilirubin showed increased levels in WT mice 3days and 7 days after BDL. (B) Haematoxylin and Eosin staining on liver sections showing profuse necrosis in livers from mice after BDL. (C,D) FACS analysis of immune cells isolated from livers showing increased numbers of CD11b/F4/80 cells in livers after BDL. (E) Histopathological analysis of colon sections showing tissue damage after BDL and (F) shortening of colon crypts (in microns). (G) Detection of FITC in serum samples from mice gavaged with FITC-Dextran 3 hours before sacrifice showing increased intestinal permeability. *P < 0.05, **P < 0.01, ***P < 0.001 [Control vs BDL].