Figure 1 | Scientific Reports

Figure 1

From: Abscisic acid induced a negative geotropic response in dark-incubated Chlamydomonas reinhardtii

Figure 1

Exogenous abscisic (ABA) acid differentially altered HCO3 uptake by C. reinhardtii in a light-intensity-dependent manner. Mid log phase (A750 = 0.3) algal cultures were immobilised in alginate gel beads containing the concentrations of ABA indicated and in (A) were incubated for 1 h under different light intensities (7.6 to 223.2 µmoles photons m−2 s−1) with bicarbonate indicator buffer in TAP media. Depletion of HCO3 in the media was monitored as the change in absorbance in the indicator buffer at 550 nm after the incubation period with conversion to the change in the concentration of HCO3 in the TAP media by reference to a standard calibration. Shown are the mean δHCO3 (mM) h−1 of n = 5 replicates with errors as the 95% confidence intervals around the means in each case. In (B) mid log phase (A750 = 0.3) algal cultures were sampled 1 h into the photoperiod and subsequently exposed for 1 h to the different light intensities indicated. Algal cells were pelleted from n = 3 replicate 25 mL aliquots of culture. Pellets were extracted and assessed for ABA content by competitive ELISA (MyBioSource Inc.). Data are shown as the mean ABA content cell−1 with error bars shown as +/− the 95% confidence interval around the mean in each case.

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