(A) Morphological difference of NPC43 cells cultured with or without the Rock inhibitor (RI) Y27632 in 2D and 3D culture conditions. (B) Percentage of number of viable NPC43 cells/colonies in 2D/3D culture in the absence of RI compared with in the presence of RI (4 μM). Numbers of viable cells were determined by MTT assay on day 3 after the treatment. (C) Comparison of number of viable cells of NPC43 cells treated with or without RI (4 μM) in response to PS1145 (32 μM). Numbers of viable cells were determined by MTT assay on day 3.