Figure 3 | Scientific Reports

Figure 3

From: Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates

Figure 3

Heterotypic and homotypic droplets display distinct microenvironments. (a) Confocal fluorescence and DIC images of polyU droplets with RP3-594 as a fluorescent probe. (1.5 mg/mL polyU, 0.5 µM RP3-AF594). (b) Confocal fluorescence images showing peptide/protein partitioning in heterotypic and homotypic RNA coacervates. (Left Panels: [RP3] = 500 µM, 0.6x polyU wt/wt, 10 mM MgCl2 - Right Panels: 1 mg/mL polyU, 500 mM MgCl2) (Fluorescent probes: 0.5 µM (GR)20-AF594, 0.5 µM α-synuclein-AF488, 0.5 µM Hsp27-AF594, 0.5 µM eGFP-MBP-AF488).

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