Figure 3 | Scientific Reports

Figure 3

From: Anthocyanins, delphinidin-3-O-glucoside and cyanidin-3-O-glucoside, inhibit immune checkpoints in human colorectal cancer cells in vitro and in silico

Figure 3

Flow cytometry plots (propidium iodide (PI) vs annexin V FITC (fluorescein isothiocyanate) and percentages of total apoptotic HCT 116 human colon cancer cells. The total of apoptotic cells is the combined percentage of early and late apototic cells, quadrants two and four, respectively. (a) Untreated. (b) Delphinidin-3-O-glucoside. (c) Delphinidin chloride. (d) Gallic acid. (e) Percent of apoptotic cells for all tested treatments. The results are expressed as mean ± SEM. Different letters indicate statistical difference among all treatments as determined by Tukey test p < 0.05. At least two independent experiments with duplicate data points were performed. D3G, delphinidin-3-O-glucoside; DC, delphinidin chloride; OX, oxaliplatin; RGC, red grape combination; UNT, untreated; UNT D, untreated DMSO. RGC was compared to UNT D; both had 0.3% DMSO.

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