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Figure 1

From: Anthocyanins, delphinidin-3-O-glucoside and cyanidin-3-O-glucoside, inhibit immune checkpoints in human colorectal cancer cells in vitro and in silico

Figure 1

Inhibition curves with standard error bars. (a) Pure anthocyanins on HCT 116 cells. (b) Pure phenolics on HCT 116 cells. (c) The 50% viability inhibition concentrations (IC50) of the pure anthocyanins and phenolics on HCT 116 cells (mean ± SEM) and their percent inhibition compared at 400 μg/mL. (d) Inhibition curves of the pure phenolic combinations on HCT 116 cells. (e) The IC50 values of the pure phenolic combinations on HCT 116 cells (mean ± SEM). (f) Inhibition curves of the pure phenolics and red grape combination on HT-29 cells. (g) The IC50 values of the pure phenolics and red grape combination on HT-29 cells (mean ± SEM). Letters indicate statistical difference between phenolics per column as determined by the Tukey test (p < 0.05). Table c presents the statistics for the IC50 inhibitions of HCT 116 cells by phenolics and anthocyanins. Table (e) presents the statistics for the IC50 inhibitions of HCT 116 cells by combination of phenolics and anthocyanins present in represented extracts. Table (g) presents the statistics for the IC50 inhibitions of HT-29 cells by phenolics and anthocyanins; statistics are compared within the table only for each column. At least two independent experiments were run with triplicate data points. *Inhibition for delphinidin chloride at 600 μg/mL was 43.9%. GA, gallic acid; DC, delphinidin chloride; D3G, delphinidin-3-O-glucoside; PB1, procyanidin B1; C3G, cyanidin-3-O-glucoside; M3G, malvidin-3-O-glucoside; RG, red grape; BL, black lentil; SH, sorghum; NA, not applicable; ND, not able to be determined.

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