In vitro adipogenic potential of SVF cells obtained from human SAT and VAT. The percentage of mature adipocytes was obtained by optical microscope analysis, upon Oil-Red O staining, of in vitro adipogenic differentiated cell cultures isolated from SAT (white columns) and VAT (grey columns) of normoglycemic (ob N), prediabetic (ob preDM) and diabetic (ob T2DM) obese patients (A,B). The data, described as mean value ± SD, was analyzed using the One Way ANOVA test followed, when statistically significant, by Fisher LSD test in (A) and Kruskal-Wallis test (Dunn’s method) in (B), *P ≤ 0.05, **P ≤ 0.01. The patients analyzed were: 18 ob N, 15 ob preDM, 13 ob T2DM in SAT and 20 ob N, 25 ob preDM, 23 ob T2DM in VAT. PPARG2 (C,D), FABP4 (E,F), ADIPOQ (G,H) mRNA levels quantified in adipogenic differentiated cell cultures obtained from SAT (white boxes, C,E,G) and VAT (grey boxes, D,F,H) of ob N, ob preDM and ob T2DM patients, normalized to 18S rRNA content, are reported as box plot graph with the lowest and highest values (wiskers), the medians (lines) and 5th and 95th percentiles (black circles outside the whiskers). Statistical analysis was performed using Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test in (C–H), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. The patients analyzed were: 17 ob N, 15 ob preDM, 12 ob T2DM in SAT and 20 ob N, 25 ob preDM, 23 ob T2DM in VAT.