Table 2 Recovery of spiked oncometabolites from pooled homogenates of FFPE or frozen tissues.

From: Quantitative Profiling of Oncometabolites in Frozen and Formalin-Fixed Paraffin-Embedded Tissue Specimens by Liquid Chromatography Coupled with Tandem Mass Spectrometry

FFPEMeasured conc. (µM), Mean (SD)Recoverya (%)CVb (%)Frozen TissueMeasured conc. (µM), Mean (SD)Recoverya (%)CVb (%)
Succinate added (µM)   Succinate added (µM)   
01.580 (0.031)  02.192 (0.142)  
LQC (0.5)2.011 (0.027)866.2LQC (0.6)2.710 (0.073)8614
HQC (4.0)6.031(0.132)1085.6HQC (6)7.988 (0.413)957.2
Fumarate added (µM)   Fumarate added (µM)   
00.16 (0.006)  01.91 (0.17)  
LQC (0.6)0.79 (0.02)1043.9LQC (0.6)2.46 (0.10)9614
HQC (8)8.16 (0.28)1003.4HQC (60)60.14 (2.68)974.6
Total 2-HG added (µM)   Total 2-HG added (µM)   
00.1170 (0.0025)  00.4822 (0.0183)  
LQC (0.04)0.1618 (0.0027)1126.0LQC (0.12)0.6023 (0.0152)10013
HQC (4.0)4.5932 (0.1501)1113.9HQC (6.0)5.9174 (0.2259)914.2
D-2HG added (µM)   D-2HG added (µM)   
00.0371(0.0028)  00.1101(0.0075)  
LQC (0.01)0.0101(0.0010)101%10%LQC (0.03)0.0283(0.0042)94%14%
HQC (4)3.9581(0.1031)99%2.6%HQC (4)3.8344(0.0456)96%1.2%
L-2HG added (µM)   L-2HG added (µM)   
00.1167(0.0031)  00.5697(0.0312)  
LQC (0.03)0.0292(0.0023)97%8.0%LQC (0.2)0.1927(0.0248)96%13%
HQC (4)3.7160(0.0767)93%2.1%HQC (4)3.5549(0.1802)89%5.1%
  1. aIndividual oncometabolites were spiked at low (i.e., ~30% of endogenous level) and high (i.e., high QC) concentrations in pooled homogenates of FFPE or frozen tissues. Recovery = (measured total concentration − measured baseline concentration)/nominal spiked concentration.
  2. bCoefficient variation from 6 replicate measurements.