Application examples. (A) Detection of elevated levels of 2HG (specifically D-2HG) in glioma FFPE specimens with known IDH1/2 mutations. (B) Correlation between D- to L-2HG ratios and total 2HG or D-2HG levels in glioma FFPE specimens. (C,D) Identification of subsets of hereditary or sporadic renal cell carcinoma frozen tissue samples with overproduction of fumarate or succinate. HLRCC, Hereditary Leiomyomatosis and Renal Cell Cancer (HLRCC); PGL/PCC, Hereditary Paraganglioma – Pheochromocytoma (PGL/PCC).