Low-temperature catalyst based Hydrothermal liquefaction of harmful Macroalgal blooms, and aqueous phase nutrient recycling by microalgae

The present study investigates the hydrothermal liquefaction (HTL) of harmful green macroalgal blooms at a temperature of 270 °C with, and without a catalyst with a holding time of 45 min. The effect of different catalysts on the HTL product yield was also studied. Two separation methods were used for recovering the biocrude oil yield from the solid phase. On comparision with other catalyst, Na2CO3 was found to produce higher yiled of bio-oil. The total bio-oil yield was 20.10% with Na2CO3, 18.74% with TiO2, 17.37% with CaO, and 14.6% without a catalyst. The aqueous phase was analyzed for TOC, COD, TN, and TP to determine the nutrient enrichment of water phase for microalgae cultivation. Growth of four microalgae strains viz., Chlorella Minutissima, Chlorella sorokiniana UUIND6, Chlorella singularis UUIND5 and Scenedesmus abundans in the aqueous phase were studied, and compared with a standard growth medium. The results indicate that harmful macroalgal blooms are a suitable feedstock for HTL, and its aqueous phase offers a promising nutrient source for microalgae.

The appearance of a dense mat of macroalgae on water bodies is a widespread phenomenon. An algal bloom is a result of accumulating algal biomass in the slow-moving lake, pond or river. Macroalgae blooms are largely filamentous, unattached forms, and are mainly green algal species found in nutrient-rich, and temperate waters. Increase of nitrogen and other micropollutants into water bodies are linked to increases in macroalgal blooms worldwide 1 .
Accumulation of macroalgae in rivers and ponds leads to microbial decomposition that may reduce dissolved oxygen in the water bodies due to algal respiration 2 . Low dissolved oxygen content in water bodies leads to a change in biodiversity and species composition 3 . Macroalgal blooms lead to a decline in the growth of non-blooming algae and also affects the diversity of plankton and zooplankton in water bodies 4 . These algal blooms affect the ecosystem by changing the quality of water, light penetration, and alteration of the food chain, and food web 5 . Various initiatives have been undertaken to deracinate the problem related to algal blooms contaminate freshwater as well as marine water 5 . Many studies have been reported on producing biofuels from microalgae 6 . However, a limited number of studies are related to the use of macroalgae for biofuel production. Toxic algal blooms can be used as a cheap raw material for microalgae cultivation because its density has extensively increased worldwide in recent years due to wastewater being discharged into water bodies 7 .
Hydrothermal liquefaction (HTL) is a process in which wet algal feedstocks at 200-400 °C temperature and 10-15 MPa pressure gets converted into four products (1) Bio-crude oil (2) Gas (3) Solid phase and (4) Aqueous phase rich in organics and nutrients 8 . During the HTL process, along with lipids, proteins and carbohydrates also get converted into biocrude oil. Therefore, the yield of oil is higher using HTL 8,9 . Thus, HTL is well suited to a variety of biomass, including bacteria, wastewater sludge, and algal biomass, which is fast-growing but have low-lipid contents. This process provides the energetic advantages by the use of wet algal biomass and the efficient separation of products over alternative techniques such as lipid isolation and transesterification, pyrolysis etc 9 . HTL is ideal for conversion of high-moisture biomass into biocrude oil because water acts as a reaction medium and thus avoids the costly phase of biomass drying 9 .
The use of a catalyst was found more to be suitable for HTL of macroalgae as compared to microalgae because it increases the conversion of carbohydrates into biocrude oil 10 . Previous studies have shown that most of the catalysts lead to a significant increase in biocrude oil. HTL process will not be economically viable if these catalysts cannot be recycled properly 11,12 . Na 2 CO 3 increases the HTL biocrude oil yield of terrestrial plants, and microalgae by promoting hydrolysis 12 .
Quality of the biocrude oil is dependent on the properties of the algal biomass. High carbon, hydrogen content and low ash, nitrogen, sulfur, and oxygen content containing biomass are considered as ideal for HTL 13 . HTL bio-crude ids dark in color, highly viscous liquid which is 10-10,000 times higher than that of conventional fuel and have a smoke-like smell 14 . High nitrogen content of algal biomass results in higher nitrogen content in HTL biocrude oil. This causes emission of toxic NOx which can be removed by the refining process. The high carbon content of algal biomass resulted in high biocrude yield by HTL process 10 . Composition of biocrude oil is mainly carbon content (71-73%), hydrogen content (7-8%), oxygen content (10-11%), nitrogen content (6-7%) and sulfur content (0-1%) which leads to less greenhouse gas emissions as compared to conventional biofuel and bioethanol 10 . Biocrude can be upgraded for the production of gasoline, jet fuel, and other fuel by using a suitable catalyst which can remove oxygen, nitrogen and double bonds 15 . However, HTL shows a negative energy balance, which is the principal disadvantage of this process. Fast heating and cooling also increase the yield of biocrude oil because of better conversion of protein, lipid, and carbohydrates into liquid fuel rather than solid or gaseous fuel 10 .
According to the energy-efficiency ratio, the use of HTL aqueous phase for microalgae cultivation shows a positive energy balance for biofuel production 16 . Recycling nutrients from wastewater could potentially fulfil the nutrients requirement for microalgae cultivation and scope to integrate the biofuel production and wastewater treatment 17 . Post-hydrothermal liquefaction aqueous phase can accumulate approximately 80% of nutrients and some organics, this provides an excellent opportunity for nutrient and carbon recycling 8 . The nutrients and carbon recycling have been investigated in some recent studies using aqueous phase of HTL 18 . These studies show that nutrients and carbon in the aqueous phase from hydrothermal liquefaction can be used for microalgae cultivation at different dilution factors (50-500 times) 19 . Several studies have been reported in the literature on nutrient cycling of HTL wastewater for microalgae cultivation, but the effect of a different catalyst on the growth of microalgae has not been reported yet. In a study, Jain et al. 7 reported that freshwater toxic algal blooms are a promising feedstock for microalgae cultivation.
The present study investigates explicitly a novel integrated method of using harmful algal blooms as biomass for energy production that synergistically combines algal blooms biofuel production using the HTL process as given in Fig. 1. This study aims to experimentally confirm the feasibility of the harmful green algal blooms for biocrude production completed in four steps. Four steps includes (1) utilization of harmful macroalgal blooms for the HTL process. (2) Increase the yield of biocrude oil using different separation methods. (3) Study the effect of different catalysts on biocrude oil yield. (4) Use of aqueous phase of HTL processes with a catalyst for the cultivation of microalgae.

Results
Analysis of macroalgal blooms. The Fig. 4) three central regions, lipid band (around 1630 cm −1 ), amide band (1401 cm −1 ) and the carbohydrate region (1100-874 cm −1 ). product yields by HtL. In this study yields of bio-crude oil by the two separation methods were investigated. In the first separation method (without soxhlet), 10.03% of biocrude oil was obtained. The results indicated that oil yield was high (14.06%) in second separation method (with soxhlet). The Soxhlet extraction method was selected and then further evaluated for their bio-crude oil yield with different catalysts for 45 min at 270 °C as given in Fig. 1. The total bio-oil was 20.10% with Na 2 CO 3 , 18.74% with TiO 2 , 17.37% with CaO, and 14.6% without a catalyst. The biochar yield was 30.12%, 30.05%, 32.09% and 35.84% for Na 2 CO 3, TiO 2, CaO, and without catalyst respectively. The total biocrude oil yield was measured by the mixing of bio-oil-1 and bio-oil-2. The significant portion of biocrude oil one was obtained from the acetone extraction of the solid phase.
Analysis of the biocrude oil obtained by HtL. The biocrude oil obtained from the macroalgal blooms have been analyzed by Gas Chromatography (GC), and NIST library was used for the identification of compounds ( Table 2). Nine main compounds were identified based on retention area % >1 during direct HTL without catalysts at 270 °C and 45 min of reaction time. The compounds such as amides derivatives, palmitic acid, phenolic compounds, and ketones derivatives, alkanes and alkenes derivatives and some furans were considered as central components of biocrude oil obtained by HTL of algal biomass 20 . Most of the components identified in biocrude oil extracted using macroalgal blooms were somewhat similar to those obtained from microalgae. However, differences in some of the compounds (Pyrrolo [1,2-a]pyrazine-1,4-dione hexahydro-3-(2-methylpropyl and   www.nature.com/scientificreports www.nature.com/scientificreports/ Phytol) were also recorded during the study. This may be due to differences in algal species, composition of the macroalgal blooms and also different GC-MS analysis procedure implemented.
HHV and eR of algal blooms biocrude oil. The primary elements present in biocrude oils obtained by the catalytic and non-catalytic reaction is given in Table 3. The biocrude oil extracted in the absence of catalyst displayed higher carbon content (70.31%) rather than the macroalgal blooms.
Element enrichment percentage was higher in biocrude oil obtained using Na 2 CO 3 as a catalyst . In the biocrude oil, the nitrogen concentration was less as compared to the green algae. This may be a result of nitrogenous compounds that are obtained in the liquid phase of the HTL process. The amount of nitrogen in the biomass of macroalgal blooms is higher than that of bio-crude oil as a result of generation of nitrogenous compounds during the HTL process in aqueous phase 21 . In contrast to our study, Ross et al. 12 reported that using Na 2 CO 3 there is an increase in nitrogen content, whereas Jena et al. 20 observed a decrease in nitrogen content of biocrude oil with Na 2 CO 3 catalyst. The sulfur content was recorded below one wt. % for Na 2 CO 3 and TiO 2 based reaction, whereas for petroleum crude oil, it varies between 0 and three wt.% 22 .

Analysis of HtL water phase.
Water phase obtained by the HTL of harmful macroalgal blooms had a very foul smell and is dark brown. The pH of catalytic reactions (7.8-8.3) was found to be higher than non-catalytic reaction (7.6) ( Table 4). The same pattern of pH was also observed in the aqueous phase of other algal biomass after HTL when Na 2 CO 3 was used as catalyst 12,22 .
The high amount of TOC present in the liquid phase of HTL is due to organic matter of feedstock dissolved in water. The nitrogen content in the HTL liquid phase increases in catalyzed reaction because algal proteins get converted into water-soluble amino acids and ammonia 23 .
Effect of catalysts stress on microalgal growth, biomass and lipid productivity. Microalgal growth in the aqueous phase of each catalyst is different; it may be due to removal and detoxification of catalyst by adsorption of catalysts on to the cell surface or intracellular metabolism of microalgae in response to catalysts. In the aqueous phase of Na 2 CO 3 and TiO 2 log phase lasted from day 6 to day 24. While in the aqueous phase of the CaO log phase lasted from day 5 to day 20 (Fig. 2). Overall microalgae, biomass productivity (g/l) after the stationary phase in the control medium (BBM) was found to decrease as compared to the HTL aqueous phase. As Fig. 2 shown that microalgae cultivated in HTL grew with the slow rate but the linear fashion after four days. Each catalyst affects the biomass and lipid productivity differently. Highest lipid yield and productivity of 32 ± 6.1% and 147 ± 1.3 mg L −1 d −1 were recorded in Scenedesmus abundansgrown in aqueous phase containing catalyst TiO 2 followed by CaO (Chorella minutissima 26.4 ± 1.7%; 157 ± 1.2 mg L −1 d −1 ), Na 2 CO 3 (Scenedesmus abundans 24.2 ± 2.1%; 110 ± 1.5 mg L −1 d −1 ), and control (Chorella minutissima 22.16 ± 1.6%; 134 ± 2.1 mg L −1 d −1 ) (Tables 5  and 6).

Discussion
FTIR spectrum of harmful macroalgal blooms reflects three central regions, lipid band (around 1630 cm −1 ), amide band (1401 cm −1 ) and the carbohydrate region (1100-874 cm −1 ). Peaks between 2516-1401 cm −1 presents the lipid and phenolic content of algal blooms 24 . Higher phenolic content was reported in marine macroalgae 25 . The sharp peak at 1639 cm −1 is C=O amide stretching of proteins present in algae 26 .
In the present study, for the first time harmful macroalgal blooms were used in HTL process for biocrude oil production. High-temperature (300-500 °C with holding time of 30-60 min) based HTL process for the conversion of biomass to bio-crude oil have been reported in previous studies 27,28 . However, the low-temperature   Table 4. Chemical characteristics of HTL aqueous phase.
(250-290) HTL process is still in the trial stage. In this study maximum, 20.1 ± 0.3% oil was obtained in the presence of catalyst Na 2 CO 3 at 270 °C for 45 min. The presence of catalysts showed the increase in oil yield and decrease in the biochar formation 29 . Catalysts increase the quality of biocrude oil by two ways (a) Introduction of catalysts at the time of the HTL process. (b) Upgrade the bio-oil quality after HTL. Catalysts from renewable resources are getting attention because they reduce the production cost of biofuels 30 . In this study, eggshell were used to produced CaO catalyst. Use of Na 2 CO 3 as a catalyst in HTL leads to increases in the yield of biocrude oil from algal blooms. The bio-oil produced with Na 2 CO 3 also had high heating value. Yeh et al. 31 reported that Na 2 CO 3 increases the yield ofbiocrude oil during the HTL of algal biomass containing a high content of carbohydrates by converting it into the oil.
In this study maximum yield of crude oil (20.10%) was reported with catalyst Na 2 CO. The presence of Na 2 CO 3 catalyst has mainly suited the conversion of carbohydrates into biocrude oil in macroalgae and plant-based     12,23 . However, Na 2 CO 3 is inimical to the conversion of algal lipids to biocrude oil and not suitable for algal species contain high lipid contents 23 . Shakya et al. 22 reported that higher amount of carbohydrates containing microalgae species led to increasing in bio-oil yield when Na 2 CO 3 is used as a catalyst. In this study, HHV and ER value of algal blooms biocrude oil with catalyst Na 2 CO 3 were 25.59MJ/Kg and 27.50% respectively. At similar pressure, Alhassan et al. 32 reported the 21.15 ± 0.82 MJ/kg and ER 41.48% of biocrude oil of Jatropha curcas cake under 250 °C temperature. The energy efficiency of HTL biocrude oil depends on the HHV 33 .
Many catalysts such as KOH, NaOH, CH 3 COOH, and H 2 SO 4 have been used by various researchers in HTL of microalgae, and these catalysts can also be reused as growth media for the growth of microalgae along with aqueous phase 12,21 . Wang et al. 27 reported that by using TiO 2 in HTL of microalgae at 300 °C leads to the highest bio-oil yield and the maximum liquefaction conversion.
The higher organic carbon content of macroalgae was responsible for the higher yield of bio oil 34 . The crude oil obtained from this study was 14-20% dw which is more similar to as reported in different green macroalgal species, Enteromorpha prolifera 23.0% 16 , Oedogonium 26.2% and Ulva 18.7% of dry weight 34 .
Algal biomass protein first to gets converted into amino acids and finally into amines and amides by HTL process 12 . Ketones and phenols produced during HTL were obtained from carbohydrates by hydrolysis and dehydration process. The lipids were responsible for the generation of alkenes 16 . Change in nitrogen concentration of aqueous phase of HTL depends on the temperature, type of catalyst, and algae strain 12,22 . High nitrogen content was reported in this study with Na 2 CO 3 as a catalyst. Similar results were observed by Shakya et al. 22 , when Na 2 CO 3 used as a catalyst in HTL of Isochrysis and Pavlova at low temperature, as Na 2 CO 3 increased hydrolysis of protein into water-soluble compounds.
The aqueous phase of HTL had a high amount of soluble organic compound, carbon, and nitrogen. Kumar et al. 35 reported that CaCl 2 increases microalgal biomass productivity. High lipid content was reported in catalysts containing aqueous phase as compared to control. These results were in congruence with other studies in which an increase in lipid content on exposure to heavy metals was reported 36 . Oxidative stress results in degradation of the photosynthetic machinery, while the protective mechanism of algal cells leads to the accumulation of unsaturated fatty acids 37 .
This experimental work confirmed that harmful macroalgal blooms are a suitable feedstock for HTL and aqueous phase can be reuse as a nutrient source for cultivation of microalgal biomass.

Materials. Microalgae strains Chorella minutissima (MCC-27), Scenedesmus abundans (NCIM 2897),
Chlorella singulari UUIND,and Chlorella sorokiniana UUIND6 were used in present study. All the chemicals, including TiO 2 and Na 2 CO 3 and solvents used, were HPLC grade and acquired from Himedia, India. proximate analysis of green macroalgal blooms. Green macroalgal blooms were locally collected during the winter season (December-February 2017) from water pond, and freshwater river nearby Uttaranchal University, Uttarakhand, India. The ash content of algal blooms biomass was determined according to the NREL Analytical Procedure 38 . Elemental compositions of algal blooms were determined by the elemental analyzer (Thermo Fisher, USA). The proximate analysis of the sample was carried out using the standard methods given by the Association of Official Analytical Chemists (AOAC). FTIR analysis (FTIR 6700, NICOLET) of algal blooms biomass frequency range 4000-450 cm −1 were used. experimental procedure of hydrothermal liquefaction (HtL). The Hydrothermal liquefaction (HTL) of harmful macroalgal blooms was carried out in a 100 ml high-pressure autoclave (Parr reactor) operated in a batch mode. Different types of catalysts in different concentrations were used in HTL process reported in the literature, but 10:1 (feedstock: catalyst) ratio has been reported to give the maximum conversion rate of feedstock to crude oil by HTL 27,39 . The reactor was loaded with wet algal blooms with/without catalyst in 10:1 ratio. Three types of catalysts were used in this study viz; TiO 2 , Na 2 CO 3, and CaO. The CaO catalyst was prepared from eggshells according to the protocol given by Niju et al. 40 . The reactor was heated up to 270 °C and pressure 4.5 MPa with He gas, a heating rate of 5 °C/min for holding time of 45 min. After the completion of reaction, the reactor was immediately cooled and opened; gas was vented off, water phase and solid mixture were separated from each other by vacuum filtration. The filtrate was marked as an aqueous phase which consists of dissolved organic compounds. Two separation methods were used for recovering these products.
In the first separation method, the solid phase was treated with acetone three times to recover the oil phase. Acetone was evaporated at 60 °C, and the resulting oil phase was weighed and marked as Biocrude oil 1. The water phase was treated with diethyl ether. Aspirating out upper phase and diethyl ether was evaporated in a rotary evaporator. The total biocrude oil obtained was measured gravimetrically and marked as biocrude oil 2.
In the second separation, Karagoz et al. 29 method were used with some modificationa, briefly the solid phase was acidified to pH 1-2 with H 2 SO 4 (1.3 M) overnight and dried next day. Biocrude oil from the solid mixture was extracted using soxhlet extraction apparatus with acetone as solvent until the solvent in the thimble becomes colorless. Allowed to stand for three h, upper pahse was separated out. The lower phase (pallets) contain catalyst or residues of catalyst. Acetone was recovered from the upper phase at 60 °C. The extracted oil phase was weighed and marked as biocrude oil-1. Diethyl ether was added to liquid phase and aspirating out upper phase and evaporated diethyl ether in a rotary evaporator and remaining fraction was measured gravimetrically and marked as biocrude oil-2.
Biocrude oil-1 was mixed with biocrude oil-2 for the calculation of %wt of total biocrude oil. Analysis of biocrude samples was doen using an elemental analyzer (Thermo Fisher, USA). (2019) 9:11384 | https://doi.org/10.1038/s41598-019-47664-w www.nature.com/scientificreports www.nature.com/scientificreports/ HHV and energy recovery (eR). The essential properties of HTL biocrude oil such as biocrude oil yield percentage, HHV, element enrichment %, and ER were calculated using the empirical formulas given below 27 Microalgal strains were cultivated in 1 L shake flasks containing different dilutions of the aqueous phase of catalytic and non-catalytic reaction and Bold's Basal Medium (BBM) as a control for 14 days with 16 h light: 8 h dark photoperiod and irradiated with LED tubes (200 μmol m −2 s −1 ). BBM was prepared according to the protocol developed by Guarnieri et al. 42 .
Three concentrations 200×, 400× and 600× of the aqueous phase of non-catalytic reaction were prepared by the dilution of distilled water ( Table 1). The effect on the growth rate of microalgae strains was observed by taking absorbance at 750 nm using UV-Vis spectrophotometer (Shimadzu 133 model no. 1700). Dilution 400× was capable of growing the maximum biomass and was further evaluated for their efficiency in biomass production with 1% BBM and 400× dilution of the aqueous phase of the catalytic reaction.

Determination of total lipid content (%, w/w) and lipid productivity of the cultivated microalgae.
For extraction of lipids, first of all, samples were dried from the % ml culture broth. Further, the microalgal cells were broken down using liquid nitrogen with the help of a mortar and pestle. The fine powder was obtained from which the lipids were extracted using chloroform:methanol (2:1) kept overnight at room temperature, with constant shaking 35 . The extract obtained was treated with 0.034% MgCl 2 , centrifuged at 3500 rpm for 5 min. The supernatant was washed two-three times with 1 ml of 2 N KCl/methanol (4:1 v/v). 5 ml of chloroform/methanol/ water (3:48:47, v/v/v) was added to it. The bottom chloroform layer was transferred to a new test tube, and lipids yield was measured gravimetrically. Lipid production and percentage of lipid were calculated by the following equations 35 : = × Lipid yield % Lipid content (g)/Dry algae biomass (g) 100 = × Lipid productivity Biomass productivity Lipid yield (%)/100 Statistical analysis. All the experiments were done in triplicates (n = 3) and are presented in mean value ± SD. A GraphPad Prism software (version7:0) with p < 0.05 was used in this study.