AIEE Active Nanoassemblies of Pyrazine Based Organic Photosensitizers as Efficient Metal-Free Supramolecular Photoredox Catalytic Systems

Pyrazine derivatives DIPY, TETPY and CNDIPY have been designed and synthesized which form fluorescent supramolecular assemblies in mixed aqueous media due to their AIEE and ICT characteristics. Among all the derivatives, the assemblies of TETPY and CNDIPY show strong absorption in the visible region with high absorption coefficients, low HOMO-LUMO gap, and high photostability. Further, the supramolecular nanoassemblies of TETPY and CNDIPY show excellent potential to generate reactive oxygen species (ROS) under the visible light irradiation. Owing to their strong absorption in the visible region and ROS generation ability, the supramolecular nanoassemblies of TETPY and CNDIPY act as efficient photoredox catalytic systems for carrying out (a) oxidative amidation of aromatic aldehydes (b) hydroxylation of boronic acid and (c) oxidative homocoupling of benzylamines under mild conditions such as aqueous media, aerial environment, and natural sunlight as a source of irradiation. All the mechanistic investigations suggest the participation of in-situ generated ROS in the organic transformations upon light irradiation.

supramolecular assemblies as photoredox catalytic systems for oxidative organic transformations. Since aggregation-induced emission enhancement (AIEE) materials are known for their high photostability 15 , hence, we planned to synthesize donor-acceptor systems like DIPY, TETPY, and CNDIPY ( Fig. 1) using AIEE active pyrazine (Py) scaffold as acceptor moiety and terpyridine (Tpy) as donor group(s). Besides the high photostability, we envisaged that terpyridine and pyrazine based donor-acceptor systems will exhibit strong absorption in the visible region due to intramolecular charge transfer (ICT) transition. Amazingly, all the pyrazine derivatives show ICT and AIEE characteristics and the assemblies of TETPY and CNDIPY were found to exhibit strong absorption in the visible region with high molar extinction coefficients and low HOMO-LUMO gap (E g ) as compared to the assemblies of DIPY derivative. All the derivatives show remarkable photostabilities and high potential to generate superoxide radicals/singlet oxygen in the aggregated state when exposed to visible light radiation in comparison to their isolated forms. Interestingly, the as-prepared supramolecular assemblies of pyrazine derivatives TETPY and CNDIPY exhibit high photocatalytic activity in; (a) oxidative coupling of benzylamines (b) additive/ base free oxidative amidation of aldehydes and (c) hydroxylation of boronic acid in mixed aqueous media under mild conditions using natural sunlight as the source of irradiation. The present photoredox catalytic systems are found to be better than the other catalytic systems reported in the literature (Comparison Tables S1-S3 in the Supplementary). Most importantly, in all the reactions, low catalytic loading (0.1 mol%) of the photoredox system was used.
To best of our knowledge, this is the first report which demonstrates the development of AIEE-ICT active 'metal/heavy atom' free supramolecular nanoassemblies of pyrazine based photosensitizers as efficient photoredox catalytic systems for carrying out oxidative organic transformations using natural sunlight as the source of irradiation.
The CNDIPY was synthesized in 80% yield by Suzuki Miyaura coupling between bromo derivative of dicyanopyrazine (3) 18  photophysical and electrochemical behavior of pyrazine derivatives. We examined the photophysical behavior of all the synthesized pyrazine derivatives by UV-vis and fluorescence studies. Two absorption bands appear in the UV-vis spectra of all the derivatives. The absorption band in the range of 290-330 nm is attributed to the π -π* transitions of pyrazine core 19 and the second absorption band observed at longer wavelength (370-400 nm) is attributed to the intramolecular charge transfer (ICT) transitions ( Fig. 2A and Table 1). Both TETPY and CNDIPY derivatives exhibit relatively strong absorption in the visible region. Further, to confirm the ICT characteristics of these derivatives, we carried out the fluorescence studies in different solvents. Interestingly, all the derivatives show positive solvatochromic behavior (Figs S1-S3 in the Supplementary) which confirms their ICT characteristics. To examine the aggregation induced enhanced emission (AIEE) characteristics of the synthesized compounds, we carried out the fluorescence studies of pyrazine derivatives in different fractions of DMSO/H 2 O solvent mixtures. Upon gradual addition of water upto 80% to DMSO solution of CNDIPY, a five-fold enhancement in the intensity of the emission band at 460 nm is observed (Fig. 2B). Likewise, derivative TETPY (2.1 fold) and DIPY (4.9 fold) show emission enhancement upon addition of water fraction to their DMSO solution (Figs S4,S5 in Supplementary). The viscosity dependent fluorescence studies of DIPY, TETPY and CNDIPY derivatives in DMSO/glycerol solvent mixture show the enhancement in the emission intensity which confirms that restriction to intramolecular rotation is the reason behind the AIEE phenomenon (Figs S6-S8 in the Supplementary). After examining the AIEE characteristics of the pyrazine derivatives, their photostability was also checked and for this, we exposed the DMSO: H 2 O solutions of all the derivatives to continuous visible irradiation for 36 h and monitored the change in their absorption behavior using UV-vis spectroscopy. To our pleasure, no significant change in the absorption behavior was observed in case of all the derivatives even after 36 h of continuous exposure.
These results highlight the remarkable photostability of supramolecular assemblies of all the pyrazine derivatives (Figs S9-S11 in the Supplementary) We further examined the morphology of TETPY and CNDIPY derivatives by transmission electron microscopy (TEM). The TEM images of TETPY derivative show the presence of spherical shaped assemblies having the size in a range of 50-100 nm while irregular assemblies with size in the range of 50-100 nm were observed in case of CNDIPY derivative. (Fig. S12A,B in Supplementary). The size of nanoassemblies was also determined by dynamic light scattering (DLS) experiments which corroborated very well with TEM studies (Fig. S12C in Supplementary). The energy dispersive X-ray (EDX) spectrum of TETPY derivative shows the absence of any metallic content (Fig. S13 in Supplementary).   Next, we examined the electrochemical behavior of all the pyrazine derivatives to determine their HOMO-LUMO energy gaps (E g ) 18 . We observed the tuning of the energy gaps with the change in the acceptor strength (CNDIPY) and donor strength/extent of conjugation (TETPY). From the cyclic voltammetric studies, CNDIPY showed the smallest HOMO-LUMO gap which may be attributed to its strong push-pull behavior (Table 1) (Fig. S14 in the Supplementary). The low E g values of these materials emphasize their potential to generate radicals through a single electron transfer (SET) mechanism.
The HOMO-LUMO gap was also evaluated using density functional theory (DFT) calculations and these results very well corroborated the experimental studies (Figs S15-S17 in the Supplementary).
Next, we evaluated the role of pyrazine assemblies to act as photosensitizers by examining their potential in the transportation of electrons from donor to acceptor moiety. For carrying out these studies, methyl viologen (MV 2+ ) and triethanolamine (TEOA) were chosen as the electron acceptor and sacrificial donor materials, respectively. Upon addition of TEOA (50 mM) to the solution of supramolecular assemblies of TETPY in DMSO: H 2 O (1:1), a 30% decrease in the intensity of the absorption band was observed. This observation suggests the existence of strong interactions between a sacrificial donor and supramolecular assemblies of TETPY. Thereafter, we added MV 2+ (0.2 mM) to the solution and exposed it to the day-light under an inert atmosphere. The color of the solution changed from colorless to dark blue after 4 minutes. The absorption spectrum of the solution in the presence of methyl viologen showed the formation of two new bands at 395 and 603 nm, respectively. These two bands are due to the presence of reduced cationic species ⋅+ MV of methyl viologen (Fig. 3A). The intensity of both bands gradually increased upon exposure to day-light. These observations led us to propose that assemblies of TETPY are reductively quenched by a sacrificial donor to generate radical anion (TETPY *− ) which in turn transports electron to MV 2+ and reduce it (Fig. 3B) 20 . The blue colored radical cation persisted for 2 h and was slowly reoxidized upon exposure to aerial conditions. Similarly, supramolecular assemblies of CNDIPY also showed the color change within five minutes from colorless to blue (Fig. S18 in the Supplementary). These results demonstrate the potential of the supramolecular assemblies of TETPY and CNDIPY to act as efficient photosensitizers. Thereafter, we evaluated the potential of the synthesized materials to generate reactive oxygen species (ROS) and for this we employed 9,10-anthracenediyl-bis(methylene) dimalonic acid (ABDA) as a singlet oxygen scavenger. The ability of ABDA to get consumed upon reaction with in-situ released singlet oxygen by photosensitizer upon irradiation is well documented [21][22][23][24] . The absorption range of ABDA is very close to that of assemblies of pyrazine derivatives. To rule out the possibility of self-quenching of absorption of ABDA due to its simultaneous excitation upon exposure to radiation, we performed a blank experiment by exposing the solution of ABDA to light radiation for 20 minutes. No significant change in the absorption of ABDA was observed 25 (Fig. S20 in the Supplementary). These results encouraged us to use ABDA as an oxygen scavenger to determine the ROS generation potential of the synthesized materials. The solution of CNDIPY (5 µM) in DMSO: H 2 O (1:1) containing ABDA was aerated for 5 minutes before its exposure to radiation. After aeration, the solution was exposed to light radiation (λ = 400 nm) for 2 minutes and its absorption was monitored using UV-vis spectroscopy.
This procedure was repeated several times to achieve saturation (20 minutes). The absorption studies show a 60% decrease in absorption intensity of ABDA upon total exposure to radiation for 20 minutes (Fig. 4A,B). However, a 35% consumption of ABDA was observed when the experiment was carried out in its isolated state (DMSO solution of CNDIPY) (Fig. S21 in the Supplementary). These studies show that AIEE active supramolecular nanoassemblies of CNDIPY have more photosensitizing ability in aggregated state than in the isolated state.
The nanoassemblies of TETPY [5 µM, in DMSO: H 2 O (1:1)] consumed 50% of the total ABDA upon exposure to light radiation (λ = 400 nm) for 20 minutes and only 25% of total ABDA was consumed in the isolated form of TETPY derivative (Figs S22 and S23 in the Supplementary). However, in the presence of DIPY nanoassemblies, a 20% decrease in the absorption intensity of ABDA was observed after total exposure of 20 minutes (Fig. S24 in the Supplementary). Overall, the fast rate of consumption of ABDA in the presence of supramolecular assemblies of CNDIPY and TETPY show their good potential towards the generation of singlet oxygen. Further, we carried out the cyclic voltammetric studies of all the synthesized derivatives to determine their potential to reduce the molecular oxygen to superoxide radical. Both TETPY and CNDIPY derivatives show sufficient reduction potential (Table 1)  possess all the desirable characteristics of photoredox catalytic systems due to their excellent photostability, low HOMO-LUMO gap, high absorption coefficient in the visible region, and good ROS generation abilities. Keeping this in view, we planned to examine the oxidative coupling of benzylamines using supramolecular assemblies of pyrazine derivatives as photoredox catalytic systems. The selective oxidation of amines to the corresponding imines is one of the most significant functional group conversions since imines and their derivatives are important building blocks for the synthesis of biomolecules, pharmaceuticals and valuable chemicals 29,30 . In literature, a variety of photosensitizers based on commercially available organic dyes, BODIPY, MOF, and polymers have been used for the oxidative coupling of the benzylamines [29][30][31][32] . The utilization of heavy atoms in organic photosensitizers, costly metal-based complexes in MOFs, tedious synthetic routes of COFs, and polymers restrict their large scale photocatalytic applications. Moreover, in most of the cases blue LED is utilized as the source of irradiation. Recently, zinc-based MOFs have been utilized for carrying out homocoupling of benzylamines using NIR LED 33,34 . Though the utilization of NIR light is advantageous, however, the heating (60 °C) is needed for the longer period (24 h) to get good yields of the product which actually decreases the 'green' benefits of the approach (Fig. 6).
In the present investigation, we carried out the model reaction of benzylamine in acetonitrile (ACN) under tungsten bulb irradiation and aerial conditions using assemblies of TETPY as photoredox catalytic systems  www.nature.com/scientificreports www.nature.com/scientificreports/ (Fig. 7). To our pleasure, the reaction proceeded well and the desired product was obtained in 82% yield ( Table 2, entry 1). When the model reaction was carried out under natural sunlight, the desired product was obtained in 85% yield ( Table 2, entry 2).
These results indicate that the assemblies of TETPY act more efficiently under irradiation of natural sunlight. Hence, we utilized natural sunlight as a source of irradiation for carrying out the next organic transformations. We repeated the model reaction using TETPY in its isolated form as photocatalysts, the desired product was obtained in only 68% yield ( Table 2, entry 3). The model reaction was also performed with CNDIPY in its aggregated and isolated form and the target products were obtained in 85% and 65% yields, respectively (Table 2, entries 4, 5). These results show that supramolecular assemblies of pyrazine derivatives have more photocatalytic efficiency than their monomeric analogs. The desired product was obtained in 45% yield in case of assemblies of DIPY (Table 2, entry 6). The low catalytic efficiency in case of assemblies of DIPY may be attributed to their low absorption in the visible region. Further, we examined the effect of different solvents in oxidative homocoupling reaction of benzylamines by switching the solvents from DCM, ACN: H 2 O, DMSO to THF and in all the cases the desired product was furnished in lower yields (Table 2, entries 7-10).
We performed a series of experiments to achieve the optimized conditions and all the control experiments ( Table 2, entries [11][12][13] show that presence of assemblies of pyrazine derivative as photocatalysts, natural sunlight, aerial conditions, and ACN as solvent are essential for achieving the good yields. With optimized conditions in hand, we examined the catalytic activity of assemblies of TETPY toward various substituted benzylamines and in all the cases the desired product was obtained in good yields (Fig. 8).
To get insight into the mechanistic pathways, we carried out the model reaction using assemblies of TETPY as photocatalysts in the presence of singlet oxygen scavengers such as sodium azide 35 and DABCO 36 , the desired product was obtained in 30% and 28% yields, respectively. The model reaction was also carried out with superoxide scavenger such as 1,4 benzoquinone 37 using assemblies of TETPY as photocatalysts and the yield of the desired product decreased to 38%. (Fig. S27 in the Supplementary).
On the basis of these observations, we propose that singlet oxygen and superoxide radicals generated by assemblies of pyrazine derivative upon photoexcitation oxidized the benzylamine (A) to the imine derivative (C) with the release of H 2 O 2 . The imine derivative (C) reacted with another molecule of benzylamine to generate the desired product (D) (Fig. 9) 30 .
To validate the assumption of the release of H 2 O 2 as by-product, we checked the in-situ generation of H 2 O 2 by adding the N,N-diethyl-1,4-phenylenediammonium sulphate (DPD) and Horseradish peroxide (POD) in the reaction mixture.
We envisaged that POD will catalyze the oxidation of DPD in presence of oxidizing agent i.e H 2 O 2 and the chemical change will be clearly visible to naked eye 32,38 . Amazingly, the color of the reaction mixture turned pink which is attributed to the formation of DPD radical cation. The whole experiment was monitored by UV-vis studies which supports the above assumption (Fig. 10).   www.nature.com/scientificreports www.nature.com/scientificreports/ oxidative amidation of aromatic aldehydes. The ability of supramolecular assemblies of TETPY and CNDIPY to efficiently catalyze the oxidative coupling of benzylamines motivated us to study the oxidative amidation of aromatic aldehydes using these assemblies as photoredox catalytic systems. The amide bond is a structural backbone of proteins and peptides and is also predominant in medicinally important compounds, natural products and polymers 39 . In literature, organometallic complexes, organic dyes, and triplet photosensitizers have been utilized for carrying out oxidative amidation of aromatic aldehydes using organic solvents, blue LED and in presence of additives and base [40][41][42][43] . Recently, light-mediated cross dehydrogenating coupling of aldehydes using iridium-based photocatalysts in presence of BrCCl 3 as an additive has been reported (Fig. 11) 40 . In the present case, we chose reaction between 4-nitrobenzaldehyde and pyrrolidine using DMSO: H 2 O (1:1) as the model system under natural sunlight and aerial conditions. The model reaction catalyzed by assemblies of TETPY was monitored under the irradiation of natural sunlight and aerial conditions (Fig. 12). The desired product was obtained in 90% yield using nanoassemblies of TETPY after 8 h (Table 3, entry 1) in the absence of any additives and base. Further, we utilized the nanoassemblies of CNDIPY as photocatalysts under the optimized reaction conditions and the product was obtained in 85% yield (Table 3,     www.nature.com/scientificreports www.nature.com/scientificreports/ When the model reaction was repeated in the presence of assemblies of DIPY as photocatalysts, the desired product was obtained in 45% yield (Table 3, entry 3). We studied the effect of different solvents such as DMSO, DMF, dioxane, THF, ACN, water, and DMSO: H 2 O (Table 3, entries 4-9) using assemblies of TETPY. The maximum yield was obtained in case of DMSO: H 2 O as the solvent system and low yield was obtained in H 2 O. We believe that low solubility of reactants, as well as a catalyst in H 2 O, is the reason behind the low yield of the target product. We chose DMSO: H 2 O as the solvent system to carry out further organic transformations. We also examined the effect of presence/absence of the base in the oxidative amidation of aromatic aldehydes under natural sunlight irradiation (Table 3, entry 10). The presence or absence of base did not affect the rate and yield of reaction. The reaction was also carried out in the absence of TETPY and only 8% of the desired product was obtained ( Table 3, entry 11). However, in the absence of light, the formation of desired product was not observed (Table 3, entry 12). The model reaction was also studied in an inert atmosphere and the desired product was obtained only in traces (Table 3, entry 13). These observations led us to conclude that the presence of photocatalyst, light and aerial conditions are the prerequisite to carry out the oxidative amidation of the aromatic aldehydes.
Next, we examined the catalytic efficiency of supramolecular assemblies of TETPY using different aromatic aldehyde. Both aromatic aldehydes bearing electron withdrawing and electron donating groups furnished the desired products in excellent yields (Fig. 13).

Mechanistic studies.
To gain insight into the mechanism of amidation, several control experiments were performed. The model reaction in the presence of singlet oxygen scavengers such as sodium azide 35 and DABCO 36 using TETPY as photocatalysts under optimized conditions furnished the desired products in 40% and 38% yields respectively. Further, the model reaction in the presence of 1,4-benzoquinone 37 (superoxide quencher)    www.nature.com/scientificreports www.nature.com/scientificreports/ furnished the desired product in only 38% yield (Fig. S28 in the Supplementary). These results reveal that the reaction proceeds through the intermediary of both singlet oxygen and superoxide radicals. We also checked the effect of the H 2 O 2 on the reaction kinetics by adding H 2 O 2 to the reaction mixture. The yield of the desired product under optimized conditions increased to 95% in the presence of H 2 O 2.
These control experiments indicate that during reaction conditions, H 2 O 2 is generated and in-situ generated H 2 O 2 is promoting the reaction in the forward direction. Further, in-situ generation of H 2 O 2 was also supported by POD catalyzed the oxidation of DPD (Fig. S29 in the Supplementary) 38 .
On the basis of these results, we propose the mechanism in which the TETPY on photoexcitation and ISC goes to triplet state TETPY*. This excited TETPY* transfers energy to the dioxygen to generate singlet oxygen ( 1 O 2 ). This 1 O 2 reacts with amine to give intermediate (B) with the generation of H 2 O 2 . On the other hand, the amine transfers the electron to TETPY* in its triplet excited state to generate TETPY − . which further transfers electron to dioxygen to give superoxide radical. This superoxide radical reacts with radical cation (A) to generate intermediate (B) with the generation of H 2 O 2. The in-situ generated H 2 O 2 acts as an oxidant and is responsible for the oxidative amidation of aromatic aldehyde under the natural sunlight irradiation to give the desired product (D) through the formation of (C) (Fig. 14) 44 . oxidative hydroxylation of boronic acids. Next, we explored the catalytic activity of assemblies of TETPY and CNDIPY in the hydroxylation of aryl boronic acids for the preparation of phenols. Development of new synthetic methods for preparation of phenol is important as phenol is a ubiquitous structural unit found in a number of natural products and pharmaceuticals 45,46 . Under the category of 'metal-free' catalytic system, Rose Bengal has been utilized for carrying out hydroxylation of boronic acid under blue light irradiation (Fig. 15), however the reaction conditions need 5 mol% of the catalysts along with 40 mol% of the base and toxicity of organic dye restricts their real-time application 47 .
To overcome these problems, we planned to carry out the hydroxylation of boronic acids using supramolecular assemblies of TETPY and CNDIPY as photocatalysts. We chose 4-formyl phenylboronic acid as reactant, N,N-diisopropylethylamine (DIPEA) as electron donor and supramolecular assemblies of TETPY as photocatalysts (Fig. 16) to furnish the desired product in 88% yield using ACN: H 2 O as a solvent system (Table 4, entry 1).  www.nature.com/scientificreports www.nature.com/scientificreports/ The model reaction was also carried out with assemblies of CNDIPY as photocatalysts to furnish the product in 80% yield ( In order to check the role of each component, we carried out hydroxylation in the absence of photocatalyst/ light radiation and in both cases, no product formation was detected (Table 4, entries 10, 11). Further, we carried out the hydroxylation of 4-formylphenylboronic acid using assemblies of TETPY as photocatalysts under an inert atmosphere, however, the desired product was obtained in trace amount only (Table 4, entry 12). These results show that the reaction is being promoted by in-situ generated ROS by assemblies of TETPY derivative upon the natural sunlight irradiation under aerial conditions.
With optimized conditions in hand, the substrate scope was explored with regard to different aryl boronic acids. The aryl boronic acids with electron withdrawing groups furnished the desired product in relatively higher yields in comparison to the aryl boronic acids having electron donating groups (Fig. 17).
To confirm the role of ROS, the model reaction was carried out in the presence of singlet oxygen scavengers such as sodium azide 36 and DABCO 37 using TETPY as photocatalysts to furnish the desired products in 65% and 58% yields respectively (Fig. S30 in the Supplementary).The reaction was also carried out in the presence of    www.nature.com/scientificreports www.nature.com/scientificreports/ superoxide scavenger such as 1,4-benzoquinone 38 and the product was obtained in 20% yield. These studies show that superoxide radical generated through single electron transfer (SET) is the key participant in the reactions.
Based upon the experimental results and literature reports, we propose the plausible mechanism for the hydroxylation of boronic acid using assemblies of TETPY as photocatalyst under natural sunlight irradiation as shown in Fig. 18 48 . The TETPY upon photoexcitation and ISC comes to triplet excited state TETPY*. Superoxide radical is being generated through the electron transfer process from DIPEA through the formation of TETPY − . under natural sunlight irradiation. The superoxide radical reacts with arylboronic acid to form (B). Intermediate (B) abstracted the proton from (A) gives the formation of (C). Aryl migration takes place after the removal of hydroxyl anion from intermediate (C) to generate (D). Hydrolysis of intermediate (D) gives the target product (E).

conclusion
We designed and synthesized supramolecular nanoassemblies of pyrazine based donor-acceptor systems DIPY, TETPY, and CNDIPY which form supramolecular nanoassemblies in mixed aqueous and have AIEE and ICT characteristics. The assemblies of TETPY and CNDIPY show strong absorption in the visible region with high absorption coefficients, high ROS generation abilities, low HOMO-LUMO gaps, and high photostabilities. The assemblies of TETPY and CNDIPY act as efficient photocatalysts for carrying out (a) oxidative amidation of aromatic aldehydes (b) hydroxylation of boronic acid and (c) oxidative homocoupling of benzylamines using mild conditions such as aqueous media, aerial condition and natural sunlight as a source of irradiation. All the mechanistic investigations prove the participation of in-situ generated ROS in the completion of the organic transformations.   www.nature.com/scientificreports www.nature.com/scientificreports/ TETPY and CNDIPY) in DMSO: H 2 O (1:1) mixture containing ABDA was aerated for 5 min before irradiation. The irradiation was done in a fluorescence spectrophotometer equipped with a Xe lamp at the excitation wavelength of 400 nm for 2 min. Then the absorbance of the solution was recorded with UV-vis spectrophotometer. This experiment was repeated several times and the total exposure time was 20 min. The blank experiment was performed by giving exposure of radiation to the solution of ABDA at 400 nm for 20 minutes.

Determination of superoxide generation ability.
To a solution of pyrazine derivatives (DIPY/TETPY/ CNDIPY) (5.0 µM) in DMSO: H 2 O (1:1) mixture, the 50 µM solution of N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) was added under aerobic conditions and the solution was kept under irradiation of visible light conditions 5-20 minutes (depending on pyrazine derivatives) 26 . The color and absorbance change of the solution were recorded using UV-vis spectrophotometer.
the DpD control experiments for oxidative coupling of benzylamine and oxidative amidation of aromatic aldehydes. The DPD (N,N-diethytl-1,4-phenylenediammonium sulphate) and POD (Horseradish peroxide) were added to the reaction mixtures (oxidative coupling of benzylamines and oxidative amidation of aromatic aldehydes) in the presence of TETPY photocatalyst/without photocatalyst. The mixture was kept for stirring under aerial conditions in sunlight 32,38 . As the reaction proceeds, the H 2 O 2 released in-situ and oxidizes DPD to DPD radical cation catalyzed by POD with intense pink coloration. The absorbance of the solution was monitored with UV-vis spectrophotometer.