RNA-sequencing of parental and resistant OVSAHO cells indicate heterozygous mutations at codon 470 in resistant cells but not in parental cells. (A) Two different mutations (E470K4 and E470D1) in resistant cells. Mutated bases are indicated by arrows. (B) Less than 4% of transcripts (148 out of 4204 reads) contain insertional mutations at codon 616. (C,D) Genomic and cDNA sequencing indicates efficient editing of codon 470 by CRISPR-based gene editing. Mutated bases are indicated by the red arrowhead. (E) HEK-293T cells harboring observed mutations at codon 470 are more resistant than parental cell1. (F) Clone #4 harboring E470K (E470K4) and Clone #1 harboring E470D (E470D1) show attenuated UPR upon CB-5083 treatment. The western blot images were taken from the same blot but are cropped to remove the irrelevant lanes.