Table 1 Funnel Strategy −2,532 compounds screened.

From: Identifying chemopreventive agents for obesity-associated cancers using an efficient, 3D high-throughput transformation assay

Hit Parameter Identified Hits Hit Percentage
Transformation HTS −>50% inhibition 178 7.0
Cytotoxicity −<25% decrease in HEK293 cell viability) 105 4.2
Promiscuity - active in <20% assays listed in Pubchem 58 2.3
Commercially available compounds - DTP/CC 33 1.3
Fresh powder confirmation −>40% inhibition 7 0.27
  1. Abbreviations: HEK293, human embryonic kidney; DTP, Developmental Therapeutics Program; CC, Cayman Chemical.