A paradigm shifting study demonstrated that induction of MHC class E and II-restricted CD8+ T cells was associated with the clearance of SIV infection in rhesus macaques. Another recent study highlighted the presence of HIV-1-specific class II-restricted CD8+ T cells in HIV-1 patients who naturally control infection (virus controllers; VCs). However, questions regarding class II-restricted CD8+ T cells ontogeny, distribution across different HIV-1 disease states and their role in viral control remain unclear. In this study, we investigated the distribution and anti-viral properties of HLA-DRB1*0701 and DQB1*0501 class II-restricted CD8+ T cells in different HIV-1 patient cohorts; and whether class II-restricted CD8+ T cells represent a unique T cell subset. We show that memory class II-restricted CD8+ T cell responses were more often detectable in VCs than in chronically infected patients, but not in healthy seronegative donors. We also demonstrate that VC CD8+ T cells inhibit virus replication in both a class I- and class II-dependent manner, and that in two VC patients the class II-restricted CD8+ T cells with an anti-viral gene signature expressed both CD4+ and CD8+ T cell lineage-specific genes. These data demonstrated that anti-viral memory class II-restricted CD8+ T cells with hybrid CD4+ and CD8+ features are present during natural HIV-1 infection.
There are nearly 37 million people living with HIV-1, and an estimated 1.8 million new HIV-1 infections were reported in 20171. Although combination antiretroviral therapy (ART) has helped to effectively control HIV-1 replication, prevent the development of AIDS, prolong life and reduce the risk of transmission, there are a still a number of limitations involved in providing this lifelong therapy to people living with HIV-12. These limitations, which include drug resistance, drug toxicity, limited lifelong patient adherence, maintenance of a latent HIV-1 reservoir and the economic costs of providing drugs to resource poor nations (which account for over 70% of people living with HIV-1), highlight the need to identify an effective means of controlling the virus in the absence of ART1,2. As a result, large-scale efforts and resources are being directed towards vaccine research and development.
The hallmark of an effective viral vaccine is to elicit robust, effective and extensive humoral and cellular immune responses against the virus. Within the context of HIV-1, broadly neutralizing antibodies (bNabs) that prevent infectivity, non-neutralizing antibodies with Fc-mediated antiviral activity, and cytotoxic CD8+ T cells that eliminate virus-infected cells have emerged as three major elements that regulate viral clearance3,4,5,6,7,8. Optimism for T cell-based vaccines received a major boost with the report of a non-human primate (NHP) T cell-based vaccine study that resulted in the induction of a novel subset of MHC class II and class E-restricted CD8+ T cells9,10. These novel subsets of CD8+ T cells were associated with complete clearance of SIV in 50% of animals11,12. Surprisingly, the majority of the epitopes targeted by these protective CD8+ T cells were MHC Class II-restricted and the remainder were MHC-E restricted; challenging the conventional MHC-I restricted CD8+ T cell paradigm9,13. These findings were remarkable because natural infection with HIV-1 or SIV usually induces T cell responses that decrease virus replication in acute infection but fail to eradicate the virus13,14. This work highlights a potential role for unconventional MHC class II-restricted CD8+ T cells in controlling viremia. Further analysis of elicitation of these atypical CD8+ T cells in humans by other pathogens will elucidate whether they are an attractive target for induction by vaccine strategies.
Although rarely reported, the presence of unconventional MHC class II-restricted CD8+ T cells has been reported in mice and human studies15,16,17,18,19,20,21,22,23. CD4-deficient, MHC class II-deficient mice or CD4-deficient mice with a transgenic class II-specific T Cell Receptor (TCR) provide the most evidence for unconventional MHC class II-restricted CD8+ T cells elicited by either bacterial or viral infections in mice15,16,17,18,19, although there is some evidence for very low frequency unconventional CD8+ T cells in wild-type B6 mice18. In humans, HLA class II-restricted CD8+ T cells were previously observed in human transplant recipients who generated allo-reactive CD8+ T cells20,21,22,23, indicating a possible role of these unconventional cells in allo-recognition. It has been suggested that the highly inflammatory and immunogenic conditions following transplantations might be involved in the misdirected lineage instruction of these HLA class II-restricted CD8+ T cells20. In the context of viral infection, cytolytic effector memory HIV-1-specific class II-restricted CD8+ T cells have also recently been observed in a small subset of HIV-1 virus controller (VC) patients who naturally control viremia in the absence of any form of therapy24. However, it is still not clear whether this unique cell population is present in any other cohorts. Given these infrequent observations of HLA class II-restricted CD8+ T cells in humans, it is important to identify other cohorts with these cellular subsets, to elucidate their functional role and investigate the factors responsible for the induction of these cells. In addition, due to their association with HIV-1 clearance in non-human primates, it is especially important to further investigate the functional role of HLA class II-restricted CD8+ T cells in the setting of HIV-1 infection.
HIV-1 VCs represent a rare subset of HIV-1-infected individuals (<1%) that can maintain undetectable HIV-1 RNA levels without therapy25. We and others have previously shown that CD8+ T cells from VCs have an enhanced ability to inhibit HIV-1 replication in infected autologous CD4+ T cells through both soluble anti-viral factors and contact dependent, target cell killing14,26,27,28,29,30,31. We have also shown that this potent VC CD8+ T cell antiviral activity is augmented by enhanced mRNA stability and transcription of antiviral cytokines30,31, and also the presence of multifunctional and highly proliferative HIV-1 specific CD4+CD8+ double positive T cells32. Thus, the VC patient cohort provides us with a human model of HIV-1 virus replication suppression that we can use to study CD8+ T cell mediated suppression of HIV-1 and also investigate the presence and functional role of HLA class II-restricted CD8+ T cells in HIV-1+ patients.
The aim of this study was to determine the presence of unconventional class II-restricted CD8+ T cells in a southern U.S. HIV-1 virus controller cohort and to define their anti-viral gene expression signature and relationship to the CD4+ T cell lineage. We first determined the frequency, memory phenotype and viral suppressive ability of these HLA class II-restricted CD8+ T cells in different cohorts (seronegative healthy donors, VCs and chronic patients) to understand whether different disease states affected induction/maintenance, frequency and memory phenotype of these cells. Using ex vivo cells from HIV-1 VCs with a consistent presence of class II-restricted CD8+ T cells at multiple time points during the course of infection, we examined the nature of these rare cells through analysis of their anti-viral gene expression signature, TCR repertoire diversity, and expression of T cell lineage-specific transcription factors representative of ontogeny. These findings define the presence of unconventional anti-viral HIV-1 Gag-specific class II-restricted CD8+ T cells with a distinct transcriptional profile characterized by the expression of both CD4 and CD8-lineage specific genes.
Primary human CD8+ T cells can inhibit virus replication through both HLA Class I and Class II recognition
In an effort to investigate the possible functional role of HLA class II-restricted CD8+ T cells in HIV-1 viral control, we chose to look at the nature of the potent anti-HIV-1 CD8+ T cell responses in HIV-1 VCs with broad CD8+ T cell mediated anti-HIV-1 inhibitory activity14,26,30,31. VC patients with a viral load below 5,000 copies/mL and a CD4+ T cell count above 400 cells/µL (Table 1) were enrolled for this study. The potency and breadth of CD8+ T cell-mediated virus inhibition was first assessed using a contact-mediated viral inhibition assay (VIA) against a panel of lab-adapted (NL4-3) and full-length subtype B (WITO, WEAU3, CH040.c, CH058.c and CH077) transmitted/founder virus strains33. Primary CD8+ T cells isolated from the peripheral blood of the VC patients were tested for HIV-1 inhibition in HLA-matched primary autologous CD4+ enriched T cells. While the magnitude of inhibitory activity varied, all the VCs tested possessed broad CD8+ mediated viral inhibitory activity against the panel of HIV-1 viruses (Fig. 1A). We next tested whether CD8+ T cell mediated HIV-1 antiviral activity was dependent on the concentration of CD8+ T cells. Anti-viral activity increased with increasing effector (CD8+): target (CD4+) ratios with a subtype B T/F virus CH058.c (Fig. 1B). The primary CD8+ T cells isolated from seronegative donors lacked antiviral activity (Fig. 1B). These results confirmed the potent HIV-1 specific antiviral response within the CD8+ T cell population of HIV-1 VC patients.
To determine whether HLA class II-restricted CD8+ T cells are part of the anti-HIV-1 cellular repertoire in chronic virus controllers, we interrogated a VC cohort (Table 1) for the presence of HLA class II-restricted CD8+ T cell antiviral activity capable of suppressing T/F virus CH058.c replication in autologous CD4+ T cells. An MHC blocking virus inhibition assay (VIA) was used in which infected primary CD4+ T cells were treated with either 3F10 (anti-MHC class I monoclonal antibody) or L243 (anti-MHC class II monoclonal antibody) (Supp Fig. 1) prior to incubation with autologous primary CD8+ T cells. Infection of CD4+ T cells resulted in a ~15% drop in their viability. Infected CD4+ T cell viability was not affected by the addition of the L243 and 3F10 monoclonal antibodies (Supp Fig. 2A). We found that HLA class II-restricted CD8+ T cells, capable of suppressing T/F virus CH058.c replication, were present in five out of the seven HIV-1 VC patients tested (Fig. 2). In addition to HLA class II-restricted antiviral CD8+ T cell activity, all VC subjects possessed HLA class I-restricted antiviral activity, as expected.
To test whether contact was required for the MHC blocking activity, the targets and effector cells from VC AH were separated in a transwell MHC blocking VIA (Supp Fig. 2B). Class II-blocking was abrogated, consistent with a contact dependent mechanism (Supp Fig. 2B). These data indicated that human HIV-1 VC subjects possessed HIV-1 specific HLA class II-restricted specific CD8+ T cells capable of suppressing HIV-1 replication in autologous CD4+ T cells.
Detectable low frequency of memory HLA DRB1*0701 restricted Gag293-312–specific CD8+ T cells in HIV-1 VC patients
We next sought to determine the frequency and memory phenotype of the HLA class II-restricted CD8+ T cells in HIV-1 VCs (Table 1) and also in chronic patients (Table 2) and seronegative healthy donors (Table 3).
We, and others, have previously shown that VCs and chronically infected HIV-1 donors (chronic patients) with anti-viral CD8+ activity contain predominantly Gag-specific effector CD8+ T cells30,34,35,36,37,38,39,40. Within the HIV-1 Gag-p24 region is the immune-dominant, HLA class II-restricted epitope spanning from amino acid position 293–312, Gag293-312, that is targeted in more than 90% of HIV-1 VCs and treated patients41. Thus, given the importance of Gag-specific antiviral activity, we utilized the available DRB1*0701 Gag293-312 -specific class II tetramer in those patients who were HLA DRB1*0701 positive to assess the prevalence of Gag-specific class II-restricted CD8+ T cells in HIV-1 infected subjects.
In this VC cohort, four of the seven VC patients tested in the MHC blocking experiment (VC V, VC AA, VC AD and VC AF) HLA matched the available HLA class II Gag293-312 tetramer (DRB1*0701), and of these four patients only two (VC AA and VC AD) demonstrated blockable HLA class II-restricted CD8+ T cell activity (Fig. 2). Two VCs (VC M and VC X) that had demonstrated CD8+ T cell-mediated antiviral activity against a panel of viruses (Fig. 1A) and one additional VC within the cohort (VC AX) that had not been previously screened for CD8+ T cell mediated antiviral activity were also DRB1*0701 tetramer matched and thus included in the tetramer analysis (Table 1). The seven DRB1*0701 tetramer matched VC patients (VC M, VC V, VC X, VC AA, VC AD, VC AF and VC AX) had PBMC samples from at least 2 time-points, while the available chronic viremics and seronegative donors had PBMC samples from only one time-point due to limited sample availability.
Since memory HIV-1-specific CD8+ T cells are known to be important for controlling viral replication42,43, we examined the frequency and memory phenotype of HLA DRB1-restricted CD8+T cells. CCR7+ CD45RA+ cells define naïve T cells, CCR7+CD45RA− define a central memory phenotype, CCR7−CD45RA+ define a terminally differentiated effector memory phenotype, and CCR7−CD45RA− cells define an effector memory phenotype44. To increase specificity of the class II staining, we utilized a dual staining approach (Fig. 3A) with different fluorophores for HIV-1 Gag and negative control tetramer presenting the invariant peptide, CLIP45.
Using tetramer staining of cryopreserved PBMCs, the five available HIV-1 seronegative donors did not have detectable HLA DRB1-restricted Gag293-312–specific CD8+ T cells (Figs 3B and 4). Two of the four available HIV-1+ chronic patients had a very low frequency of HLA DRB1-restricted Gag293-312–specific CD8+ T cells while the remaining two patients did not have any detectable HLA DRB1-restricted Gag293-312–specific CD8+ T cells (Figs 3C and 4). The HLA DRB1-restricted Gag293-312–specific CD8+ T cells in the chronic patients were predominantly effector memory cells, 62.5% of the HLA class II-restricted CD8+ T cells for patient 425 and 93.3% for patient 830 (Fig. 3D). Of the seven VC patients tested, only two, VC AA and VC AD, had consistent detectable HLA DRB1-restricted Gag293-312-specific CD8+ T cells across multiple visit dates during the course of their infection (Figs 3E and 4, Supp Figs 3A and 4A). These HLA DRB1-restricted Gag293-312–specific CD8+ T cells were predominantly central memory (CM) and effector memory (EM) phenotypes (VCAA = CM: 15.3-40.5%, EM: 43.6–67.2%) (VCAD = CM: 34.3–43.9%, EM: 22_44.4%) (Fig. 3F, Supp Figs 3B and 4B). We did not see any correlation between the estimated length of infection and frequency of class II-restricted CD8+ T cells or correlation between CD4+ T cell count and frequency of class II-restricted CD8+ T cells. In summary, two natural HIV-1 virus controllers had HLA DRB1-restricted Gag293-312–specific central and effector memory CD8+ T cells consistently present at low frequencies (0.0011–0.018%).
HLA DRB1*0701 restricted Gag293-312–specific CD8+ T cells are a unique T cell subset that expresses both CD4+ and CD8+ T cell-specific genes
We next asked whether these unconventional HIV-1 class II CD8+ T cells have a unique transcriptional profile compared to conventional class I CD8+ T cells and conventional class II CD4+ T cells. The very low frequency of class II- CD8+ T cells (barely at the limit of quantitation) in two of the four chronic patients tested and their absence in the healthy donors (Fig. 4) limited our gene expression analysis to the two VC patients, VCAA and VCAD, that had the highest frequencies and most consistent (across multiple time points) class II- CD8+ T cells. Gag-specific class II-restricted CD4+ T cells, Gag-specific class II-restricted CD8+ T cells and Gag-specific class I-restricted CD8+ T cells were stained, and flow sorted with class I or class II fluorophore-labeled tetramers (Supp Fig. 5) and RNA-sequencing performed on bulk sorted tetramer positive cell populations. The purity of the sorted CD4 and CD8 T cell subsets ranged from 97.1–98.9%.
HLA class II-restricted CD8+ T cells (GagIICD8) in both patients had a unique gene expression profile compared to HLA class I-restricted CD8+ T cells (SL9CD8) and HLA class II-restricted CD4+ T cells (GagIICD4) (Fig. 5A) (Table S1). Given the unique gene expression profile of HLA class II-restricted CD8+ T cells, we then evaluated the expression of known human CD4+ and CD8+ specific genes and lineage-specific transcription factors (Table 4 46,47,48). Conventional CD4+ T cells are known to be Runx3L°wThPOKHigh, while conventional CD8+ T cells are known to be ThPOKL°wRunx3High 49,50,51.
CD8+ T cell subsets (BulkCD8 - CD8+ T fraction composed of both Gag tetramer-specific and Gag tetramer-nonspecific CD8+ T cells, SL9CD8 - Gag p17-specific class I-restricted CD8+ T cells and GagIICD8 - Gag p24-specific class II-restricted CD8+ T cells) all expressed CD8-specific genes (CD8B, CD8A, CST7 and CCL5), which were all absent in the CD4+ T cell subsets (Fig. 5B). Bulk CD4+ T cells and tetramer specific CD4+ T cells (GagIICD4) all expressed CD4, while VCAD tetramer specific CD4+ T cells also expressed ZBTB7B (ThPOK) and CTSB (Fig. 5C). Interestingly, we observed that HLA class II-restricted CD8+ T cells (GagIICD8) also expressed multiple CD4-associated genes, with VCAA class II CD8s expressing ZBTB7B (ThPOK) and CTSB, while VCAD class II CD8s expressed MXI1 (Fig. 5C) (Table S2). Additionally, Gag tetramer-specific CD4+ T cells (GagIICD4) had reduced expression levels of multiple CD4-associated genes ThPOK, MXI1 and CTSB compared to the CD4+ T cell fraction composed of both Gag tetramer-specific and Gag tetramer-nonspecific CD4+ T cells (BulkCD4) (Fig. 5C).
The results from the transcriptomics analysis suggests that HLA class II-restricted CD8+ T cells from patients VCAA and VCAD, while sharing some features from both conventional CD4 and CD8+ T cells, possess some distinct features that make them a unique T cell subset that might possess distinct functional properties that could be harnessed to complement conventional CD8+ T cell-mediated HIV-1 control.
HLA DRB1*0701 restricted Gag293-312–specific CD8+ T cells have an anti-viral gene profile comparable to that of conventional class I-restricted CD8+ T cells
To test the hypothesis that Gag-specific HLA class II-restricted CD8+ T cells have a unique transcriptional profile with evidence of anti-viral gene expression, we profiled expression of known cytolytic molecules and anti-viral genes associated with CD8+ T cell-mediated anti-HIV-1 activity31,52,53,54,55 (Table 5) in HIV-1 Gag-specific HLA class II-restricted CD8+ T cells, HLA class I-restricted CD8+ T cells and HLA class II-restricted CD4+ T cells from VCAA and VCAD.
HLA class II-restricted CD8+ T cells (GagIICD8) in VCAA and VCAD had unique anti-viral gene expression profiles compared to the conventional HLA class I-restricted CD8+ T cells (SL9CD8) (Fig. 6) (Table S2). VCAA HLA class II-restricted CD8+ T cells highly expressed CCL22, IL21R, GATA3, GNLY, IL2RA and SPON2 (Fig. 6A), while VCAD HLA class II-restricted CD8+ T cells highly expressed XCL1, ITGAL, IL7R, LAMP1, IL2RA and SPON2 (Fig. 6B) compared to their respective HLA class I-restricted CD8+ T cells. SPON2 and IL2RA genes were highly expressed in HLA class II-restricted CD8+ T cells compared to HLA class I-restricted CD8+ T cells in both patients (Fig. 6). We also observed that HLA class II-restricted CD8+ T cells had lower expression of T cell activation and exhaustion markers (Table 6) compared to HLA class I-restricted CD8+ T cells (Fig. 7) (Table S2). VCAA HLA class I-restricted CD8+ T cells highly expressed CD160, TIGIT, FASLG and KLRG1 transcripts associated with chronic activation and exhaustion (Fig. 7A), while VCAD HLA class I-restricted CD8+ T cells highly expressed PDCD1 (PD1), CD160, TIGIT and KLRG1 transcripts associated with chronic activation and exhaustion (Fig. 7B) compared to their respective HLA class II-restricted CD8+ T cells. CD160, TIGIT and KLRG1 were highly expressed in HLA class I-restricted CD8+ T cells in both patients, while CTLA4 was highly expressed in HLA class II-restricted CD8+ T cells in both patients (Fig. 7). VCAA and VCAD patient-specific variations in anti-viral and exhaustion gene expression profiles were also observed.
These results demonstrate that HLA class II-restricted CD8+ T cells in VCs had lower expression of T cell activation and exhaustion markers, and a unique anti-viral gene signature compared to conventional HLA class I-restricted CD8+ T cells.
DRB1*0701 restricted Gag293-312–specific CD8+ and CD4+ T cells have distinct TCR repertoires
To gain an insight into how HLA class II-restricted CD8+ T cells respond to a given antigen, we examined the clonal diversity of HIV-1 Gag-specific HLA class II-restricted CD8+ T cells. Given that we also had HLA class II-restricted CD4+ T cells targeting the same Gag293-312 epitope, TCR repertoire analysis enabled measurement of antigen-specific T cell diversity in both HLA class II-restricted CD4 and CD8+ T cells.
Based on the Gini index which measures the skewness of the clonal distribution (with a low score associated with an equal clonal distribution while a high score is associated with repertoire skewing) from the tcR package56, analysis of the top 100 clones in VCAA and VCAD (Table S3) shows that the HLA class I-restricted CD8+ TCR repertoire was consistently narrow and biased in both patients (Fig. 8A), suggesting an oligoclonal expansion of class I-restricted CD8+ T cells in response to Gag p17. For VCAA (no protective alleles), the HLA class II-restricted CD8+ TCR repertoire was broad and diverse while the HLA class II-restricted CD4+ TCR repertoire was narrow and biased (Fig. 8A). VCAD (HLA B57*01 protective allele) had a narrow and biased HLA class II-restricted CD8+ TCR repertoire while the HLA class II-restricted CD4+ TCR repertoire was broad and diverse (Fig. 8A). These findings indicate that there is a polyclonal expansion of HLA class II-restricted CD8+ T cells compared to HLA class II-restricted CD4+ T cells in response to Gag293-312 in VCAA, while an oligoclonal expansion of HLA class II-restricted CD8+ T cells compared to HLA class II-restricted CD4+ T cells is observed in VCAD (Fig. 8A).
Given the observed clonal diversity differences between HLA class II-restricted CD8+ and CD4+ T cells targeting the same epitope, we further interrogated the clonal composition of these two subsets by comparing the highly variable Complementarity Determining Region 3 (CDR3) nucleotide sequence overlap. Vβ CDR3 nucleotide sequence overlap across the different T cell subsets, expressed as a similarity Jaccard Index score ranging from zero to one, showed low CDR3 sequence sharing and TCR repertoire similarity between HLA class II-restricted CD8+ and CD4+ T cell subsets (Fig. 8B). In summary, these results demonstrate that HLA class II-restricted CD4+ T cells and HLA class II-restricted CD8+ T cells with shared epitope specificity have distinct TCR repertoires.
CMV-vectored SIV vaccine studies reported control of infection associated with induction of unconventional MHC class E- or II-restricted CD8+ T cells in NHPs9,11,12. Studies are ongoing to analyze the specific contribution of MHC-II vs. MHC-Ia vs. MHC-E restricted CD8+ T cell responses to protection in the setting of RhCMV/SIV-mediated prophylactic vaccine protection (Louis Picker, personal communication). Ranasinghe et al. first reported that HIV-1-specific cytolytic class II-restricted CD8+ T cells are present in HIV-1 infection24. These findings have resulted in increased interest in elucidating the properties of these unconventional class II-restricted CD8+ T cells and their possible role in mediating control of HIV-1 infection. In this study, we assessed the distribution of class II-restricted CD8+ T cells in different HIV-1 patient cohorts, their relative contribution towards CD8+ T cell mediated suppression of HIV-1 replication, ontogeny, functional properties and determined TCR repertoire composition in selected individuals.
To further expand on the work done by Ranasinghe et al. and investigate whether atypical class II-restricted CD8+ T cells are only present in HIV-1 VCs, the presence of class II-restricted CD8+ T cells was interrogated in HIV-1 chronic patients, HIV-1 VCs and healthy donors. Among the three HIV-1+ patient cohorts interrogated, we observed that HIV-1+ VC patients had detectable MHC class II-restricted CD8+ T cells, while the frequency of class II-restricted CD8+ T cells were undetectable in the healthy donors and barely at the limit of quantitation in the chronic patients. The majority of VC patients exhibited contact-dependent, non-lytic, anti-viral activity which was abrogated by the addition of anti-MHC monoclonal antibodies, while one patient, VC V, exhibited contact independent non-lytic virus inhibition that was not blocked by the addition of anti-MHC antibodies. We and others have shown that CD8 T cells can mediate contact independent nonlytic virus inhibition14,30,57,58, thus potentially explaining why the high CD8+ T cell-mediated viral inhibition activity displayed by VC V was not blocked by the anti-MHC antibodies. Alternatively, VC V CTL activity could be mediated by other non-classical HLA restriction such as HLA-E which has also been reported to mediate CTL activity in humans59,60 and non- human primates10.
Thus, given our finding and that of others24, we believe that natural HIV-1 infection can induce these cells. One hypothesis is that the CD4+ T cell depletion, high viremia and inflammatory cytokine storm present during acute HIV-1 infection creates a CD4 deficient, pro-inflammatory environment61,62 that might be conducive for the expansion of these rare class II-restricted CD8+ T cells or their conversion from CD4+ T cells. This possibility is also supported by the observation that CD4-deficient mice mount strong MHC class II-restricted CD8+ T cell responses after primary bacterial or viral infection15,16,17,18,19. Additionally, reports from the transplant patients with class II-restricted CD8+ T cells suggested that the highly inflammatory and immunogenic environment might be involved in the induction of these atypical class II-restricted CD8+ T cells20. However, more studies are needed to understand the relationship between HIV-1 infection and MHC class II-restricted CD8+ T cells, and further our understanding of additional factors driving or restricting induction and detection of these cells. Moreover, we also observed that the MHC class II-restricted CD8+ T cells present during natural HIV-1 infection had predominant effector and central memory phenotypes. This finding was striking as canonically, CD8+ T cells associated with lower viral loads in chronic HIV-1 infection possess either a central memory63 or effector memory phenotype64,65 with low PD-1 expression66,67.
The presence of a class II-blockable CD8+ T cell mediated anti-viral activity was further supported by the presence of a unique anti-viral gene expression profile in Gag-specific class II-restricted CD8+ T cells. Two anti-viral genes from our down-selected list, SPON2 and IL2RA, were highly expressed in HLA class II-restricted CD8+ T cells compared to HLA class I-restricted CD8+ T cells in both patients. SPON2 is highly expressed in CD8+ T cells primed to exert effector activities55,68,69, while IL2RA expression is also required for IL-2 dependent CD8+ T cell effector responses70,71. XCL1, which was highly expressed in VCAD HLA class II-restricted CD8+ T cells inhibits a broad range of HIV-1 isolates, independent of viral co-receptor usage and genetic subtype53, while CCL22 which is highly expressed in VCAA class II-restricted CD8+ T cells is a known anti-viral β-chemokine secreted by both CD8+ and CD4+ T cells during HIV-1 infection72.
Potent CD8+ T cell antiviral activity is one of the factors thought to mediate VC status14,26,27,29,31,32. Given these findings, it is plausible that the presence of unconventional HLA class II-restricted CD8+ T cells may contribute to the CD8-mediated anti-viral activity present in VCs. MHC class II and E-restricted CD8+ T cells have been reported in the NHP model to have desirable features for potent CD8+ T cell mediated anti-viral activity. Some of these features include a broader antigen epitope recognition profile, unique targeting of supertopes and the ability to target conserved, subdominant epitopes9. Thus, it will be interesting to probe whether the HLA class II-restricted CD8+ T cells in VCs and chronic patients have some of the above unique features, and also whether the higher frequency of HLA class II-restricted CD8+ T cells observed in the VC patients might be involved in mediating their superior protective capacity.
Highlighting the ontogeny of these atypical CD8+ T cells is critical for understanding the factors that trigger induction of these cells and hence will provide valuable insight into how these cells can be targeted by vaccines and harnessed to provide protection from HIV-1 or other viral infections. In the NHP study, the rhesus CMV vector was responsible for the induction of MHC class II-restricted CD8+ T cells and not natural infection. However, the observation of unconventional CD8+ T cells in humans in our study and others20,21,24, suggests that they might also be induced without the aid of a CMV vector. Preliminary transcriptomic findings from two VC patients, VCAA and VCAD, suggest that HLA class II-restricted CD8+ T cells have a unique transcriptome and express both CD8-associated (CD8α/β, CST7 and CCL5) and CD4-associated genes (ThPOK and CTSB for VCAA, and MXI1 for VCAD). This initial observation suggests the possibility that HLA class II-restricted CD8+ T cells could be ‘ex-CD4+ T cells’ that undergo re-programming to adopt CD8+ T cell like features and hence explaining the presence of CD8-specific genes, an anti-viral gene signature usually associated with CD8+ T cells, CD4-specific genes and HLA class II-restriction.
HIV-1 infection has been reported to disrupt CD4+ T cell lineage-defining transcriptional profile73. Furthermore, the presence of peripheral lineage-intermediate T cell subsets with CD4 and CD8 surface markers/effector molecule expression patterns in numerous disease settings is not entirely unheard of74. Non-traditional CD4+ T cells with cytolytic functions75,76,77, and lineage intermediate CD4+/CD8+ double positive T cell subsets have been previously reported in HIV-1 infection78,79. We have also previously reported the presence of anti-viral and cytolytic CD4+/CD8+ double positive T cells in our HIV-1 Virus Controller patient cohort which included patients VCAA and VCAD used in this study32. However, the transcriptomics findings are not conclusive due to the limited sample size and also the need for lineage-tracing experiments in murine models to clearly define the relationship between CD4+ T cells and HLA class II-restricted CD8+ T cells. HLA class II-restricted CD8+ T cells exhibit patient-specific transcriptomic features that might be linked to the differences in viral loads, CD4+ T cell counts and other biological differences between patient VCAA and VCAD. It is also possible that these differences might be due to different induction mechanisms of HLA class II-restricted CD8+ T cells in patients VCAA and VCAD.
TCR repertoire clonal diversity analysis highlights repertoire richness and gives an indication of how different T cell subsets respond to a given antigen80. TCR diversity enables T cells to respond to multiple foreign antigens80 and in the context of HIV-1 infection this will endow the immune system with the ability to recognize circulating epitope variants. However, in the HIV-1 setting, it is not clear whether having a dominant protective CD8+ T cell clonotype or multiple subdominant clonotypes is better for HIV-1 replication control. TCR repertoire diversity of antigen-specific T cells was shown to be comparable between HIV-1-infected and HIV-1-uninfected subjects81, while clonal diversity could not distinguish elite controllers from chronic patients with the HLA-B5701 protective allele82. However, there are studies showing that HLA class I-restricted CD8+ T cells82,83,84 have narrow and biased repertoires with dominant clonotypes. Narrow and biased repertoires were also recently reported for HLA class II-restricted CD8+ and CD4+ T cells responding to the same HIV-1 Gag antigen in one VC patient24.
In our study, HLA class I-restricted CD8+ T cells from both individuals had narrow and biased TCR repertoires suggesting an oligoclonal expansion in response to the Gag p27 antigen. However, the HLA class II-restricted CD8+ and CD4+ TCR repertoires were different in the two individuals even though both T cell subsets were Gag293-312-specific and DRB1*0701-restricted. It is interesting to highlight that the one VC patient, VCAD, with a narrow and biased HLA class II-restricted CD8+ TCR repertoire (similar to that of the class I-restricted TCR repertoire) had the protective HLA-B57*01. VCAA, without protective HLA alleles, had a broad and diverse HLA class II-restricted CD8+ TCR repertoire. It is still unclear how the presence/absence of protective HLA alleles would influence HLA class II-restricted CD8+ TCR repertoire diversity as more patients need to be examined to confirm the relationship we observed in our two patients. Despite having different HLA class II-restricted CD8+ TCR repertoires, both patients still met the prespecified VC cohort criteria for enrollment. It remains to be seen how clonal diversity might affect HIV-1 replication control by both HLA class I- and HLA class II-restricted CD8+ T cells.
Even though we were able to detect the rare HLA class II-restricted CD8+ T cells in some of the HIV-1 infected individuals, this study still had a number of limitations. This study utilized the available HLA DRB1*0701 class II-restricted Gag293-312 tetramer and is therefore limited to the detection of a small fraction of the entire HIV-1-specific HLA class II-restricted CD8+ T cell subset within each individual and only for those individuals with this matching HLA haplotype. Further studies with more sensitive multimers such as dodecamers instead of tetramers, autologous optimal class II-restricted HIV-1 epitopes (other than the Gag epitope used in this study) within each individual and with other HLA alleles may detect higher frequencies of HLA class II-restricted CD8+ T cells. Further studies to characterize these cells ex vivo or through the generation of T cell lines and clones will enable characterization of this cellular subset by probing the class II-restricted breadth (other than Gag293-312) and function (functional reactivity to the target HIV-1 peptides, and ability to mediate viral suppression). Limited patient sample availability and very low to no detection of class II-restricted CD8+ T cells in chronic viremics and healthy patients limited investigating the presence of unconventional class II-restricted CD8+ T cells in a larger patient cohort and the comparative RNA seq analysis of patient samples with different disease outcomes. Future studies investigating the frequency and gene expression profile of class II-restricted CD8+ T cells in large HIV-1 patient cohorts with different disease outcomes (acute patients who later progress to become chronic viremics or acute patients who later become virus controllers, and also patients on ART-therapy (treatment-induced controllers) will help assess the importance of these cells in viremic control and also highlight any distinguishing features of these cells in different disease conditions. Additionally, due to the low frequency directly ex vivo, we examined expanded CD8 T cells for HLA class II activity. Thus, the expansion process may have impacted the phenotypes of the HLA class II-restricted Gag293-312–specific CD8+ T cells. Further studies with more sensitive multimers such as dodecamers instead of tetramers may result in the detection of a larger subset of these cells without prior expansion of the CD8+ T cell subset. However, given these limitations, it is remarkable that HLA class II-restricted CD8+ T cells were detected in these HIV-1 positive individuals and suggests that there might even be a larger subset of these atypical cells that were missed in this study. For the TCR diversity measurements, we were not able to measure the total body TCR diversity due to the complexity of this approach, but we only focused on the TCR diversity of a defined peripheral blood T cell subpopulation at one given time-point during the course of infection. Future studies will focus on utilizing T cells from lymph nodes and blood at multiple time points during course of infection so as get better snapshot of the HLA class II-restricted CD8+ T cells repertoires.
In summary, we have demonstrated the presence of atypical anti-viral memory HLA class II-restricted Gag293-312–specific CD8+ T cells with a unique transcriptional profile that includes expression of both CD4 and CD8+ T cell lineage-specific genes in HIV-1 virus controllers (VCs). The presence of these unconventional HLA class II-restricted CD8+ T cells was associated with natural HIV-1 infection since these cells were not present in HIV-1 seronegative individuals. The finding that functional HLA class II-restricted CD8+ T cells are present in HIV-1+ individuals highlights that elucidation of the ontogeny of this activity in humans can lead to a better understanding of how to induce protective CD8+ T cell immunity by vaccination.
Materials and Methods
Eleven antiretroviral therapy (ART)-naïve HIV-1-infected virus controllers (Table 1) (maintaining plasma HIV-1 loads of <5,000 RNA copies/ml and CD4+ T cell counts of >400 cells/µl) were enrolled through the Adult Infectious Diseases Clinic at Duke University Medical Center. The specimens utilized in this study were restricted to those patients that met this virus controller definition. Although the precise time of infection for these patients is unknown, the minimum length of infection (ranges from >2 to >14 years) is based on when these patients first reported to the clinic (VC M - 8 years, VC O -3 years, VC V – 3 years, VC X – 2 years, VC AA – 12 years, VC AD – 3 years, VC AF – 14 years, VC AG – 4 years, VC AH – 13 years, VC AJ – 4 years and VC AX – 11 years). Four HIV-1+ chronic patients (Table 2) and five healthy uninfected donors (Table 3) were enrolled through the Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery (CHAVI-ID). All patients were HLA typed using the next generation sequencing typing method. PBMC genomic DNA underwent PCR amplification and cDNA library preparation before allelic-level 3rd generation sequencing typing of full-length HLA genes was performed using the Illumina platform (ProImmune Ltd, Oxford, UK). Nine VCs were evaluated in the Viral Inhibition Assays (VIA) measuring the ability of CD8+ T cells to mediate suppression of multiple HIV-1 virus strains, while seven of the nine VCs from the VIA (based on sample availability) were tested for the contribution of HLA II-restriction in CD8+ T cell-mediated anti-viral activity. Sample availability and HLA haplotype (DRB1*0701) determined which samples (VCs, chronic viremics and healthy donors) were further characterized with HLA tetramers by flow cytometry. Frequency of class II-restricted CD8+ T cells and their presence at multiple time points in one patient determined which patient samples were evaluated by RNA-seq analysis. The studies were reviewed and approved by the Duke University Medical Center Institutional Review Board, and all participants provided written informed consent. All research was performed in accordance with the Duke University School of Medicine guidelines and regulations.
PBMC isolation and storage
Peripheral blood mononucleated cells (PBMCs) were isolated from whole blood using the Ficoll-Plaque Plus (GE Healthcare) density gradient centrifugation. PBMC were frozen (90% fetal bovine serum [FBS]-10% dimethyl sulfoxide) and stored in liquid nitrogen until analyzed.
Cell culture and cell subset preparation
Cryopreserved PBMCs were thawed in a 37 °C water bath, transferred to prewarmed RPMI 1640 (Gibco/Invitrogen) supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (Invitrogen) and then washed 2 times before they were examined for recovery and viability. 1 million cells per mL were activated by 50 ng/mL anti-CD3 (eBioscience) and 100 ng/mL anti-CD28 antibodies for 7 days in media containing RPMI 1640 supplemented with 20% heat-inactivated FBS, 1% penicillin-streptomycin, and 20 U/ml recombinant human interleukin-2 (IL-2) (PeproTech). For generating effectors and targets for the virus inhibition assay (VIA), activated PBMCs were separated into CD8+ and CD8− subsets after 3 days in culture using a CD8+ T cell isolation kit and CD8+ depletion beads, respectively (Miltenyi Biotech).
MHC class II and MHC Class I tetramers
HLA DRB1*0701 Gag293-312 tetramers (FRDYVDRFYKTLRAEQASQE), HLA DRB1*0701 Human CLIP87-101 tetramers (PVSKMRMATPLLMQA), and HLA-A*02:01 SL9 Gag77-85 tetramers conjugated to either PE or BV421 were obtained from the NIH Tetramer Facility (Atlanta, GA). The tetramers were used at a final concentration of 10 µg/ml.
Cell staining and flow cytometry
On day 7 of cell culture, activated PBMCs were enriched for CD8+ T cells using a CD8+ T cell isolation kit (Miltenyi Biotech) and then examined for viability. 8 × 106 CD8+ T cells were stained with a combination of either 10 µg/ml HLA DRB1*0701 Gag293-312-PE and 10 µg/ml HLA DRB1*0701 Gag293-312-BV421 tetramer or 10 µg/ml HLA DRB1*0701 Human CLIP87-101-PE and 10 µg/ml HLA DRB1*0701 Human CLIP87-101-BV421 tetramers (NIH Tetramer Facility) for 1 hr at room temperature (or 30 minutes at 37 °C for the HLA-A*02:01 SL9 Gag77-85 tetramers). The cells were then washed once with PBS/2% FBS and then resuspended. The expression of surface molecules was assessed using the following antibodies: CD3-APC-H7 (BD Biosciences), CCR7-BV605 (Biolegend), CD8-FITC (Biolegend), CD56-APC (eBiosciences), CD16-BV711 (BD Biosciences), CD4-AF700 (Biolegend), CD19-PercP-Cy5.5 (BD Biosciences), CD45RA-ECD (Beckman Coulter). The tetramer stained CD8+ T cells were then incubated with the surface marker antibodies for 30 minutes at 4°C in PBS/2% FBS. The cells were washed once with PBS/2% FBS and then stained with Live/Dead Fixable Aqua-Dead Cell Stain (ThermoFisher Scientific, Waltham, MA) for 30 minutes at 4°C in PBS (Invitrogen). The cells were washed once with PBS, and then once with PBS/2% FBS before being fixed in PBS/2% paraformaldehyde. Samples were acquired and analyzed using the LSRII flow cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star, Ashland, OR). For RNA-sequencing, BD FACS ARIA II (BD Biosciences) was used to sort tetramer stained PBMCs into 350 µL of RLT lysis buffer (Qiagen).
PBMC-extracted RNA from class II-restricted CD8+ T cells, class I-restricted CD8+ T cells, class II-restricted CD4+ T cells, bulk CD8 and CD4+ T cells from VC AA and VC AD were subjected to reverse transcription and amplification using the Smartseq ultra-low v4 kit (Takara Bio, Mountain View, CA). 200 pg of amplified cDNA was prepared for Illumina sequencing using the Nextera XT library preparation kit (Illumina, San Diego, CA). Libraries were quantified using qPCR (Kapa Biosystems, Wilmington, MA) and sequenced to a minimum depth of 25 million reads per sample (2 × 75 bp reads) on the Illumina NextSeq (NCBI Sequence Read Archive Accession number: PRJNA542922). Raw FASTQ files were merged and then trimmed using the default settings of the cutadapt Python module (http://cutadapt.readthedocs.io/en/stable/index.html). The trimmed reads were aligned to the human genome GRCh38 using STAR85. After reference alignment read counts on each gene were quantified by HTSeq86, significantly differentially expressed transcripts were determined by DESeq2 R package87, and filtering for adjusted p values of less than 0.05 as seen in Fig. 5A. For Fig. 5B through 7, heat maps were generated by comparing the Reads Per Kilobase of transcript per Million mapped reads (RPKM) using lists of known associated genes and calculating the z-scores by row.
Gene Network analysis was done using GeneMania88 and pathway analysis was performed using Ingenuity Pathway Analysis (QIAGEN Inc., https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis). MiXCR, an open source immune repertoire analysis package (https://github.com/milaboratory.mixcr), was used for paired-end read merging and extraction of TCR sequences from bulk RNA-seq data89. TCR repertoire analysis was then performed using the open source software tool tcR (https://github.com/imminfo/tcr)56.
Replication-competent virus stocks from full-length infectious molecular clones (IMCs) expressing transmitted/founder viruses (CH058.c, WITO, WEAU3, CH040 and CH077) and lab-adapted NL4.3 were generated as described14,90. Briefly, proviral DNA was transfected into 293T cells by using Fugene HD (Roche). Working stocks were amplified by passaging virus in human PBMC (American Red Cross). PBMC-derived virus supernatants were collected every 2 to 3 days and filtered through a 0.45 µm syringe filter, and titers were determined on TZM-bl cells (obtained through the NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH, from J C. Kappes, Xiaoyun Wu, and Tranzyme, Inc.)14.
Viral inhibition assay
This assay to measure the ability of CD8+ T cells to suppress HIV-1 replication in infected target CD4+ T cells has been previously described14. Briefly, CD4+ enriched target cells were infected with HIV-1 infectious molecular clones (MOI = 0.1) via 2-hour spinoculation at 1200 RPM. Following spinoculation, infected target cells were plated at 2.25 × 104 cells/well and CD8+ effector T cells were added to cultures at varying Effector:Target ratios (0.25:1, 0.5:1, 1:1, 2:1). Co-cultures were incubated at 37 °C and 5% CO2 for three days and virus production was measured by TZM-bl assay14 with a readout of relative light units (RLU).
This assay was adapted from the previously described virus inhibition assay14 to measure the ability of CD8+ T cells to suppress HIV-1 infection by MHC Class I and/or Class II recognition of infected target cells. Briefly, CD4+ enriched target cells were infected with HIV-1 infectious molecular clones (MOI = 0.1) via 2-hour spinoculation at 1200 RPM. Following spinoculation, infected target cells were plated at 2.25 × 104 cells/well and incubated for 30 minutes in the presence or absence of monoclonal antibodies targeting MHC Class I (3F10), MHC Class II (L243), or a negative isotype control (P3x63). Following the incubation, CD8+ effector T cells were added to cultures at varying Effector:Target ratios (0.125:1, 0.25:1, 0.5:1, 1:1). Co-cultures were incubated at 37 °C and 5% CO2 for three days and virus production was measured by TZMbl assay14 with a readout of relative light units (RLU).
Statistical significance of results was determined by the non-parametric Sign test performed using SAS (r) Proprietary Software 9.4 (TS1M2), Copyright (c) 2002–2012 by SAS Institute Inc., Cary, NC, USA, and the graphs were generated using GraphPad Prism 8.
The RNA-seq datasets generated during and/or analyzed during the current study are available in the NCBI Sequence Read Archive repository (SRA), Accession number PRJNA542922. All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data are available from the corresponding author on reasonable request.
UNAIDS. FACT SHEET NOVEMBER 2016, http://www.unaids.org/en/resources/fact-sheet (2016).
Murray, A. J., Kwon, K. J., Farber, D. L. & Siliciano, R. F. The Latent Reservoir for HIV-1: How Immunologic Memory and Clonal Expansion Contribute to HIV-1 Persistence. Journal of immunology (Baltimore, Md.: 1950) 197, 407–417, https://doi.org/10.4049/jimmunol.1600343 (2016).
Nabel, G. J. HIV vaccine strategies. Vaccine 20, 1945–1947, https://doi.org/10.1016/s0264-410x(02)00074-9 (2002).
Tay, M. Z. et al. Antibody-Mediated Internalization of Infectious HIV-1 Virions Differs among Antibody Isotypes and Subclasses. PLoS pathogens 12, e1005817, https://doi.org/10.1371/journal.ppat.1005817 (2016).
Tomaras, G. D. & Haynes, B. F. Advancing Toward HIV-1 Vaccine Efficacy through the Intersections of Immune Correlates. Vaccines (Basel) 2, 15–35, https://doi.org/10.3390/vaccines2010015 (2014).
Heeney, J. L. & Plotkin, S. A. Immunological correlates of protection from HIV infection and disease. Nature immunology 7, 1281–1284, https://doi.org/10.1038/ni1206-1281 (2006).
Corey, L. et al. Immune correlates of vaccine protection against HIV-1 acquisition. Science translational medicine 7, 310rv317, https://doi.org/10.1126/scitranslmed.aac7732 (2015).
Stephenson, K. E. & Barouch, D. H. A global approach to HIV-1 vaccine development. Immunological reviews 254, 295–304, https://doi.org/10.1111/imr.12073 (2013).
Hansen, S. G. et al. Cytomegalovirus vectors violate CD8+ T cell epitope recognition paradigms. Science (New York, N.Y.) 340, 1237874, https://doi.org/10.1126/science.1237874 (2013).
Hansen, S. G. et al. Broadly targeted CD8(+) T cell responses restricted by major histocompatibility complex E. Science (New York, N.Y.) 351, 714–720, https://doi.org/10.1126/science.aac9475 (2016).
Hansen, S. G. et al. Profound early control of highly pathogenic SIV by an effector memory T-cell vaccine. Nature 473, 523–527, https://doi.org/10.1038/nature10003 (2011).
Hansen, S. G. et al. Immune clearance of highly pathogenic SIV infection. Nature 502, 100–104, https://doi.org/10.1038/nature12519 (2013).
Ranasinghe, S. & Walker, B. D. Programming CMV for vaccine vector design. Nat Biotechnol 31, 811–812, https://doi.org/10.1038/nbt.2688 (2013).
Freel, S. A. et al. Initial HIV-1 antigen-specific CD8+ T cells in acute HIV-1 infection inhibit transmitted/founder virus replication. Journal of virology 86, 6835–6846, https://doi.org/10.1128/JVI.00437-12 (2012).
Kirberg, J. et al. Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor. The Journal of experimental medicine 180, 25–34, https://doi.org/10.1084/jem.180.1.25 (1994).
Matechak, E. O., Killeen, N., Hedrick, S. M. & Fowlkes, B. J. MHC class II-specific T cells can develop in the CD8 lineage when CD4 is absent. Immunity 4, 337–347, https://doi.org/10.1016/S1074-7613(00)80247-2 (1996).
Shimizu, T. & Takeda, S. CD8 T cells from major histocompatibility complex class II-deficient mice respond vigorously to class II molecules in a primary mixed lymphocyte reaction. Eur J Immunol 27, 500–508, https://doi.org/10.1002/eji.1830270222 (1997).
Tyznik, A. J., Sun, J. C. & Bevan, M. J. The CD8 population in CD4-deficient mice is heavily contaminated with MHC class II-restricted T cells. The Journal of experimental medicine 199, 559–565, https://doi.org/10.1084/jem.20031961 (2004).
Heemskerk, M. H. et al. Activation of virus-specific major histocompatibility complex class II-restricted CD8+ cytotoxic T cells in CD4-deficient mice. Eur J Immunol 25, 1109–1112, https://doi.org/10.1002/eji.1830250438 (1995).
Hirosawa, T. et al. Mismatched human leukocyte antigen class II-restricted CD8(+) cytotoxic T cells may mediate selective graft-versus-leukemia effects following allogeneic hematopoietic cell transplantation. Cancer Sci 102, 1281–1286, https://doi.org/10.1111/j.1349-7006.2011.01949.x (2011).
Rist, M., Smith, C., Bell, M. J., Burrows, S. R. & Khanna, R. Cross-recognition of HLA DR4 alloantigen by virus-specific CD8+ T cells: a new paradigm for self-/nonself-recognition. Blood 114, 2244–2253, https://doi.org/10.1182/blood-2009-05-222596 (2009).
Heemskerk, M. H. et al. Dual HLA class I and class II restricted recognition of alloreactive T lymphocytes mediated by a single T cell receptor complex. Proceedings of the National Academy of Sciences of the United States of America 98, 6806–6811, https://doi.org/10.1073/pnas.111162298 (2001).
Lopez, D., Barber, D. F., Villadangos, J. A. & Lopez de Castro, J. A. Cross-reactive T cell clones from unrelated individuals reveal similarities in peptide presentation between HLA-B27 and HLA-DR2. Journal of immunology (Baltimore, Md.: 1950) 150, 2675–2686 (1993).
Ranasinghe, S. et al. Antiviral CD8+ T Cells Restricted by Human Leukocyte Antigen Class II Exist during Natural HIV Infection and Exhibit Clonal Expansion. Immunity 45, 917–930, https://doi.org/10.1016/j.immuni.2016.09.015 (2016).
Deeks, S. G. & Walker, B. D. Human immunodeficiency virus controllers: mechanisms of durable virus control in the absence of antiretroviral therapy. Immunity 27, 406–416, https://doi.org/10.1016/j.immuni.2007.08.010 (2007).
Hancock, G. et al. Identification of effective subdominant anti-HIV-1 CD8+ T cells within entire post-infection and post-vaccination immune responses. PLoS pathogens 11, e1004658, https://doi.org/10.1371/journal.ppat.1004658 (2015).
Saez-Cirion, A. et al. HIV controllers exhibit potent CD8 T cell capacity to suppress HIV infection ex vivo and peculiar cytotoxic T lymphocyte activation phenotype. Proceedings of the National Academy of Sciences of the United States of America 104, 6776–6781, https://doi.org/10.1073/pnas.0611244104 (2007).
Shasha, D. et al. Elite controller CD8+ T cells exhibit comparable viral inhibition capacity, but better sustained effector properties compared to chronic progressors. Journal of leukocyte biology 100, 1425–1433, https://doi.org/10.1189/jlb.4A0915-422R (2016).
Yan, J. et al. HIV-specific CD8+ T cells from elite controllers are primed for survival. Journal of virology 87, 5170–5181, https://doi.org/10.1128/JVI.02379-12 (2013).
Freel, S. A. et al. Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination. Journal of virology 84, 4998–5006, https://doi.org/10.1128/JVI.00138-10 (2010).
Payne, T. L. et al. Transcriptional and posttranscriptional regulation of cytokine gene expression in HIV-1 antigen-specific CD8+ T cells that mediate virus inhibition. Journal of virology 88, 9514–9528, https://doi.org/10.1128/JVI.00802-14 (2014).
Frahm, M. A. et al. CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection. Journal of immunology (Baltimore, Md.: 1950) 188, 4289–4296, https://doi.org/10.4049/jimmunol.1103701 (2012).
Keele, B. F. et al. Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection. Proceedings of the National Academy of Sciences of the United States of America 105, 7552–7557, https://doi.org/10.1073/pnas.0802203105 (2008).
Kalams, S. A. et al. Association between virus-specific cytotoxic T-lymphocyte and helper responses in human immunodeficiency virus type 1 infection. Journal of virology 73, 6715–6720 (1999).
Klein, M. R. et al. Kinetics of Gag-specific cytotoxic T lymphocyte responses during the clinical course of HIV-1 infection: a longitudinal analysis of rapid progressors and long-term asymptomatics. The Journal of experimental medicine 181, 1365–1372 (1995).
Johnson, R. P. et al. HIV-1 gag-specific cytotoxic T lymphocytes recognize multiple highly conserved epitopes. Fine specificity of the gag-specific response defined by using unstimulated peripheral blood mononuclear cells and cloned effector cells. Journal of immunology (Baltimore, Md.: 1950) 147, 1512–1521 (1991).
Gea-Banacloche, J. C. et al. Maintenance of large numbers of virus-specific CD8+ T cells in HIV-infected progressors and long-term nonprogressors. Journal of immunology (Baltimore, Md.: 1950) 165, 1082–1092 (2000).
Julg, B. et al. Enhanced anti-HIV functional activity associated with Gag-specific CD8 T-cell responses. Journal of virology 84, 5540–5549, https://doi.org/10.1128/jvi.02031-09 (2010).
Masemola, A. et al. Hierarchical targeting of subtype C human immunodeficiency virus type 1 proteins by CD8+ T cells: correlation with viral load. Journal of virology 78, 3233–3243 (2004).
Saez-Cirion, A. et al. Heterogeneity in HIV suppression by CD8 T cells from HIV controllers: association with Gag-specific CD8 T cell responses. Journal of immunology (Baltimore, Md.: 1950) 182, 7828–7837, https://doi.org/10.4049/jimmunol.0803928 (2009).
Vingert, B. et al. HIV Controller CD4+ T Cells Respond to Minimal Amounts of Gag Antigen Due to High TCR Avidity. PLoS Pathog. 6, e1000780, https://doi.org/10.1371/journal.ppat.1000780 (2010).
Masopust, D. Developing an HIV cytotoxic T-lymphocyte vaccine: issues of CD8 T-cell quantity, quality and location. J Intern Med 265, 125–137, https://doi.org/10.1111/j.1365-2796.2008.02054.x (2009).
Koup, R. A. & Douek, D. C. Vaccine design for CD8 T lymphocyte responses. Cold Spring Harb Perspect Med 1, a007252, https://doi.org/10.1101/cshperspect.a007252 (2011).
Sallusto, F., Lenig, D., Förster, R., Lipp, M. & Lanzavecchia, A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401, 708–712, https://doi.org/10.1038/44385 (1999).
Day, C. L. Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers. 112, 831–842, https://doi.org/10.1172/jci200318509 (2003).
Palmer, C., Diehn, M., Alizadeh, A. A. & Brown, P. O. Cell-type specific gene expression profiles of leukocytes in human peripheral blood. BMC Genomics 7, 115, https://doi.org/10.1186/1471-2164-7-115 (2006).
Awong, G., Herer, E., La Motte-Mohs, R. N. & Zuniga-Pflucker, J. C. Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature. BMC Immunol 12, 22, https://doi.org/10.1186/1471-2172-12-22 (2011).
Tokunaga, T. et al. Regulation of Th-POK and Runx3 in T cell development in human thymoma. Autoimmunity 42, 653–660, https://doi.org/10.3109/08916930903120941 (2009).
Cheroutre, H. & Husain, M. M. CD4 CTL: living up to the challenge. Semin Immunol 25, 273–281, https://doi.org/10.1016/j.smim.2013.10.022 (2013).
Vacchio, M. S. et al. A ThPOK-LRF transcriptional node maintains the integrity and effector potential of post-thymic CD4+ T cells. Nature immunology 15, 947–956, https://doi.org/10.1038/ni.2960 (2014).
Vacchio, M. S. & Bosselut, R. What Happens in the Thymus Does Not Stay in the Thymus: How T Cells Recycle the CD4+-CD8+ Lineage Commitment Transcriptional Circuitry To Control Their Function. Journal of immunology (Baltimore, Md.: 1950) 196, 4848–4856, https://doi.org/10.4049/jimmunol.1600415 (2016).
Cocchi, F. et al. Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells. Science (New York, N.Y.) 270, 1811–1815 (1995).
Guzzo, C. et al. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1. PLoS pathogens 9, e1003852, https://doi.org/10.1371/journal.ppat.1003852 (2013).
Simonetta, F. et al. High eomesodermin expression among CD57+CD8+ T cells identifies a CD8+ T cell subset associated with viral control during chronic human immunodeficiency virus infection. Journal of virology 88, 11861–11871, https://doi.org/10.1128/jvi.02013-14 (2014).
Greenough, T. C. et al. A Gene Expression Signature That Correlates with CD8+ T Cell Expansion in Acute EBV Infection. Journal of immunology (Baltimore, Md.: 1950) 195, 4185–4197, https://doi.org/10.4049/jimmunol.1401513 (2015).
Nazarov, V. I. et al. tcR: an R package for T cell receptor repertoire advanced data analysis. BMC Bioinformatics 16, 175, https://doi.org/10.1186/s12859-015-0613-1 (2015).
DeVico, A. L. & Gallo, R. C. Control of HIV-1 infection by soluble factors of the immune response. Nature reviews. Microbiology 2, 401–413, https://doi.org/10.1038/nrmicro878 (2004).
Tomaras, G. D. et al. CD8+ T cell-mediated suppressive activity inhibits HIV-1 after virus entry with kinetics indicating effects on virus gene expression. Proceedings of the National Academy of Sciences of the United States of America 97, 3503–3508, https://doi.org/10.1073/pnas.070521097 (2000).
Schulte, D. et al. The HLA-E(R)/HLA-E(R) genotype affects the natural course of hepatitis C virus (HCV) infection and is associated with HLA-E-restricted recognition of an HCV-derived peptide by interferon-gamma-secreting human CD8(+) T cells. The Journal of infectious diseases 200, 1397–1401, https://doi.org/10.1086/605889 (2009).
Pietra, G. et al. HLA-E-restricted recognition of cytomegalovirus-derived peptides by human CD8+ cytolytic T lymphocytes. Proceedings of the National Academy of Sciences of the United States of America 100, 10896–10901, https://doi.org/10.1073/pnas.1834449100 (2003).
McMichael, A. J., Borrow, P., Tomaras, G. D., Goonetilleke, N. & Haynes, B. F. The immune response during acute HIV-1 infection: clues for vaccine development. Nat Rev Immunol 10, 11–23, https://doi.org/10.1038/nri2674 (2010).
Roberts, L. et al. Plasma cytokine levels during acute HIV-1 infection predict HIV disease progression. AIDS 24, 819–831, https://doi.org/10.1097/QAD.0b013e3283367836 (2010).
Burgers, W. A. et al. Association of HIV-specific and total CD8+ T memory phenotypes in subtype C HIV-1 infection with viral set point. Journal of immunology (Baltimore, Md.: 1950) 182, 4751–4761, https://doi.org/10.4049/jimmunol.0803801 (2009).
Hess, C. et al. HIV-1 specific CD8+ T cells with an effector phenotype and control of viral replication. Lancet 363, 863–866, https://doi.org/10.1016/S0140-6736(04)15735-8 (2004).
Addo, M. M. et al. Fully differentiated HIV-1 specific CD8+ T effector cells are more frequently detectable in controlled than in progressive HIV-1 infection. PloS one 2, e321, https://doi.org/10.1371/journal.pone.0000321 (2007).
Trautmann, L. et al. Upregulation of PD-1 expression on HIV-specific CD8+ T cells leads to reversible immune dysfunction. Nat Med 12, 1198–1202, https://doi.org/10.1038/nm1482 (2006).
Day, C. L. et al. PD-1 expression on HIV-specific T cells is associated with T-cell exhaustion and disease progression. Nature 443, 350–354, https://doi.org/10.1038/nature05115 (2006).
Hertoghs, K. M. et al. Molecular profiling of cytomegalovirus-induced human CD8+ T cell differentiation. The Journal of clinical investigation 120, 4077–4090, https://doi.org/10.1172/jci42758 (2010).
Ayano, M. et al. Increased CD226 Expression on CD8+ T Cells Is Associated with Upregulated Cytokine Production and Endothelial Cell Injury in Patients with Systemic Sclerosis. Journal of immunology (Baltimore, Md.: 1950) 195, 892–900, https://doi.org/10.4049/jimmunol.1403046 (2015).
Best, J. A. et al. Transcriptional insights into the CD8(+) T cell response to infection and memory T cell formation. Nature immunology 14, 404–412, https://doi.org/10.1038/ni.2536 (2013).
Depper, J. M. et al. Interleukin 2 (IL-2) augments transcription of the IL-2 receptor gene. Proceedings of the National Academy of Sciences of the United States of America 82, 4230–4234 (1985).
Pal, R. et al. Inhibition of HIV-1 infection by the beta-chemokine MDC. Science (New York, N.Y.) 278, 695–698 (1997).
Riou, C. et al. HIV Skews the Lineage-Defining Transcriptional Profile of Mycobacterium tuberculosis-Specific CD4+ T Cells. Journal of immunology (Baltimore, Md.: 1950) 196, 3006–3018, https://doi.org/10.4049/jimmunol.1502094 (2016).
Overgaard, N. H., Jung, J. W., Steptoe, R. J. & Wells, J. W. CD4+/CD8+ double-positive T cells: more than just a developmental stage? Journal of leukocyte biology. 97, 31–38, https://doi.org/10.1189/jlb.1RU0814-382 (2015).
Johnson, S. et al. Cooperativity of HIV-Specific Cytolytic CD4 T Cells and CD8 T Cells in Control of HIV Viremia. Journal of virology 89, 7494–7505, https://doi.org/10.1128/jvi.00438-15 (2015).
Soghoian, D. Z. et al. HIV-specific cytolytic CD4 T cell responses during acute HIV infection predict disease outcome. Science translational medicine 4, 123ra125–123ra125, https://doi.org/10.1126/scitranslmed.3003165 (2012).
Soghoian, D. Z. & Streeck, H. Cytolytic CD4(+) T cells in viral immunity. Expert review of vaccines 9, 1453–1463, https://doi.org/10.1586/erv.10.132 (2010).
Weiss, L. et al. Persistent expansion, in a human immunodeficiency virus-infected person, of V beta-restricted CD4+CD8+ T lymphocytes that express cytotoxicity-associated molecules and are committed to produce interferon-gamma and tumor necrosis factor-alpha. The Journal of infectious diseases 178, 1158–1162 (1998).
Kitchen, S. G. et al. CD4 on CD8(+) T cells directly enhances effector function and is a target for HIV infection. Proceedings of the National Academy of Sciences of the United States of America 101, 8727–8732, https://doi.org/10.1073/pnas.0401500101 (2004).
Qi, Q. et al. Diversity and clonal selection in the human T-cell repertoire. Proceedings of the National Academy of Sciences of the United States of America 111, 13139–13144, https://doi.org/10.1073/pnas.1409155111 (2014).
Baum, P. D. et al. Blood T-cell receptor diversity decreases during the course of HIV infection, but the potential for a diverse repertoire persists. Blood 119, 3469–3477, https://doi.org/10.1182/blood-2011-11-395384 (2012).
Mendoza, D. et al. HLA B*5701-Positive Long-Term Nonprogressors/Elite Controllers Are Not Distinguished from Progressors by the Clonal Composition of HIV-Specific CD8(+) T Cells. Journal of Virology 86, 4014–4018, https://doi.org/10.1128/JVI.06982-11 (2012).
Ndhlovu, Z. et al. Magnitude and kinetics of CD8+ T cell activation during hyperacute HIV infection impacts viral set point. Immunity 43, 591–604, https://doi.org/10.1016/j.immuni.2015.08.012 (2015).
Pantaleo, G. et al. Major expansion of CD8+ T cells with a predominant V beta usage during the primary immune response to HIV. Nature 370, 463–467, https://doi.org/10.1038/370463a0 (1994).
Dobin, A. et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics (Oxford, England) 29, 15–21, https://doi.org/10.1093/bioinformatics/bts635 (2013).
Anders, S., Pyl, P. T. & Huber, W. HTSeq–a Python framework to work with high-throughput sequencing data. Bioinformatics 31, 166–169, https://doi.org/10.1093/bioinformatics/btu638 (2015).
Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15, 550, https://doi.org/10.1186/s13059-014-0550-8 (2014).
Montojo, J. et al. GeneMANIA Cytoscape plugin: fast gene function predictions on the desktop. Bioinformatics (Oxford, England) 26, 2927–2928, https://doi.org/10.1093/bioinformatics/btq562 (2010).
Bolotin, D. A. et al. MiXCR: software for comprehensive adaptive immunity profiling. Nat Methods 12, 380–381, https://doi.org/10.1038/nmeth.3364 (2015).
Ochsenbauer, C. et al. Generation of transmitted/founder HIV-1 infectious molecular clones and characterization of their replication capacity in CD4 T lymphocytes and monocyte-derived macrophages. Journal of virology 86, 2715–2728, https://doi.org/10.1128/JVI.06157-11 (2012).
This work was supported by R01/R56 NIH grant AI-52779 (G.D.T.), an NIH F31 fellowship (1F31AI106519-01) (T.L.P.), Center for HIV-1/AIDS Vaccine Immunology and Immunogen Discovery (Grant UMI-AI100645), and the Center for AIDS Research (P30 AI 64518), and the Duke University Ruth and A. Morris Williams Award. We thank Sarah Mudrak, and Derrick Goodman for project management; Mike Archibald and Yue Chen for technical expertise; Nathan Vandergrift and Wes Rountree for statistical advice; Richard Scearce for antibody reagents; NIH Tetramer Core Facility for the provision of the HLA class I and class II tetramers; Thad Gurley and Tarra Von Holle for flow cytometry cell staining; Drs. Coleen Cunningham and John Bartlett, of the Duke CFAR Clinical Core, Clinical Core Staff (Stuart Carr and Sunita Patil) and the patients, physicians, and staff of the Duke Adult Infectious Diseases Clinic (Gary Cox, Nathan Thielman, Cameron Wolfe, Elizabeth Livingston, Brianna Norton, Kristen Dicks, Mehri McKellar, Vivian Chu, Jason Stout, and Ann Mosher) for virus controller patient recruitment and Dr. Myron Cohen and the CHAVI cohort clinical staff and patients. We thank Dr. John Kappes, Christina Ochsenbauer, and the UAB CFAR Virology Core for HIV-1 infectious molecule clones.
The authors declare no competing interests.
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Nyanhete, T.E., Frisbee, A.L., Bradley, T. et al. HLA class II-Restricted CD8+ T cells in HIV-1 Virus Controllers. Sci Rep 9, 10165 (2019). https://doi.org/10.1038/s41598-019-46462-8
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