Author Correction: Development of new method to enrich human iPSC-derived renal progenitors using cell surface markers

Correction to: Scientific Reports https://doi.org/10.1038/s41598-018-24714-3, published online 23 April 2018

In Figure 2C, the qRT-PCR data for SIX2 and SALL1 were miscalculated. As a result, the graphs of SIX2 and SALL1 expression are incorrect. The correct Figure 2 appears below as Figure 1.

Figure 1

The expression of renal lineage markers in CD9⁻CD140a⁺CD140b⁺CD271⁺ cells isolated from hiPSC differentiation culture. (A) Anti-GFP (OSR1), SIX2 and HOXD11 immunostaining images of the cells before isolation (upper panels) and isolated CD9⁻CD140a⁺CD140b⁺CD271⁺ cells (lower panels) on culture days 28 (SIX2) and 30 (OSR1 and HOXD11). Representative images from three independent experiments are shown. Scale bars, 100 μm. (B) The induction rates of SIX2+ cells in day 28 cells before and after isolation. The data from three randomly chosen fields are presented as the mean ± SE (n = 3). (C,D) qRT-PCR analyses of gene expressions in day 28 cells before and after isolation for markers of nephron progenitors (C) and undifferentiated cells (D). The data from three independent experiments are presented as the mean ± SE (n = 3). GAPDH was used as an internal control, and the relative expression levels were normalized to those of day 28 cells before isolation (C) and hiPSCs (D). ITGA8, integrin alpha 8; CDH11, cadherin 11.

Additionally in Figure 4C, the qRT-PCR data for Col4a1 were also miscalculated. As a result, the graph of Col4a1 expression is incorrect. The correct Figure 4 appears below as Figure 2.

Figure 2

The evaluation of kidney fibrosis after cell therapy using hiPSC-derived CD9⁻CD140a⁺CD140b⁺CD271⁺cells in mouse acute kidney injury (AKI) models (A) Representative images of host mouse kidney sections stained with anti-alpha smooth muscle actin (α-SMA) immunostaining and Masson’s trichrome (MT) and Sirius red (SR) stainings in saline- (upper panels) or CD9⁻CD140a⁺CD140b⁺CD271⁺ cell (iPSC-RP, lower panels)-treated groups. The boxed areas are magnified and displayed in the right panels. Scale bars, 500 μm in the panel to the farthest left and 100 μm in the others. (B) Quantitative analyses of kidney fibrosis areas by anti-α-SMA immunostaining and MT and SR stainings in the host kidneys on day 12 after I/R injury (n = 4). Statistical significance: *P < 0.05 vs. saline after multiple testing adjustment. (C) qRT-PCR analyses of gene expression in the host kidneys on day 12 after I/R injury for the markers of kidney fibrosis (n = 4). Statistical significance: *P < 0.05 vs. saline after multiple testing adjustment. Fsp1, Fibroblast-specific protein 1; Col4a1, alpha-1 subunit of collagen type IV, NS, not significant.