Figure 3 | Scientific Reports

Figure 3

From: Reversine exhibits antineoplastic activity in JAK2V617F-positive myeloproliferative neoplasms

Figure 3

Reversine decreases proliferation, clonogenicity, and cell cycle progression in SET2 and HEL cells. (A) Ki-67 mean fluorescence intensity (M.F.I.) was determined by flow cytometry after incubation of SET2 or HEL cells treated with reversine (1, 2.5, 5, 10 μM) for 24 hours; histogram traces are illustrated. The bar graphs represent the Ki-67 M.F.I normalized to the respective untreated control cells, and results are shown as mean ± SD of at least four independent experiments. The p values and cell lines are indicated in the graphs; *p < 0.05, **p < 0.01, ***p < 0.001; ANOVA test and Bonferroni post-test. (B) Colonies containing viable cells were detected by MTT after 10 days of culture of SET2 and HEL cells treated reversine (1, 2.5, 5, 10 μM) for 36 hours and normalized to the corresponding DMSO-treated controls (Ø). Colony images are shown for one experiment and the bar graphs show the mean ± SD of at least four independent experiments. ***p < 0.0001; ANOVA test and Bonferroni post-test. (C) Cell cycle progression was determined by PI staining in SET2 or HEL cells treated with reversine (1, 2.5, 5, 10 μM) for 24 hours. A representative histogram for each condition is illustrated. (D) Bar graphs represent the mean ± SD of the percent of cells in G0/G1, S and G2/M phase upon reversine treatment of at the least four independent experiments. The p values and cell lines are indicated in the graphs; *p < 0.05, **p < 0.01, ***p < 0.001; ANOVA test and Bonferroni post-test.

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