Offset responses are significantly stronger in AAF than in A1. (a) Raster plot of the example A1 (top-left) and AAF (top-right) neuron’s response to 9 kHz PTs played at 60 dB SPL with sound duration varying between 50 and 500 ms and ISI between 50 and 2000 ms and (bottom) PSTH (mean ± SD) averaged over all neurons population. The blue shaded bars represent the tone. (b) Comparison of strength of onset and offset responses in A1 and AAF for 500 ms 9 kHz PTs played with 2000 ms ISIs. Responses are color-coded to onset BF. (c) A1 (left) and AAF (right) offset responses to 9 kHz PTs with increasing duration across all ISIs’ (correlation between sound duration and response rate: A1, ρ = 0.1429, p = 0.8028, n = 191; AAF, ρ = 1, **p = 0.0028, n = 190, Spearman correlation). (d) Ratio of offset/onset responses in A1 and AAF for 9 kHz PTs with varying durations (50–500 ms) and 2000 ms ISI. Data represent mean ± SEM. ****p < 0.0001, ***p = 0.0007, Mann-Whitney test. (e) Onset responses of A1 (left) and AAF (right) neurons evoked by 9 kHz PTs of different durations (ISIs varied between 50 and 2000 ms). (f) Comparison of A1 and AAF onset responses to 9 kHz PTs (500 ms duration) with ISI varied between 50 and 2000 ms. There was no significant difference in forward suppression: p = 0.5887, Mann-Whitney test. Spike rates were normalized to maximum response of each individual neuron.