Figure 3 | Scientific Reports

Figure 3

From: Caffeine exposure induces browning features in adipose tissue in vitro and in vivo

Figure 3

hMSC response to caffeine exposure during a 21-day period of adipogenic differentiation. (a) Representative images of cells stained for lipid droplets with ORO (Scale bar: 50 μm) and mean (b) staining quantified spectrophotometrically. (c) Cell viability assay. (d) Representative images showing upregulation of UCP1 (red) in hMSCs. DAPI was used to identify cell nuclei (blue) and BODIPY was used to identify lipid droplets (green); scale bar: 10 μm and (e) mean relative UCP1 abundance (n = 20). Data presented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.

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