Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

In vivo measurements reveal a single 5′-intron is sufficient to increase protein expression level in Caenorhabditis elegans


Introns can increase gene expression levels using a variety of mechanisms collectively referred to as Intron Mediated Enhancement (IME). IME has been measured in cell culture and plant models by quantifying expression of intronless and intron-bearing reporter genes in vitro. We developed hardware and software to implement microfluidic chip-based gene expression quantification in vivo. We altered position, number and sequence of introns in reporter genes controlled by the hsp-90 promoter. Consistent with plant and mammalian studies, we determined a single, natural or synthetic, 5′-intron is sufficient for the full IME effect conferred by three synthetic introns, while a 3′-intron is not. We found coding sequence can affect IME; the same three synthetic introns that increase mcherry protein concentration by approximately 50%, increase mEGFP by 80%. We determined IME effect size is not greatly affected by the stronger vit-2 promoter. Our microfluidic imaging approach should facilitate screens for factors affecting IME and other intron-dependent processes.


Introns are noncoding DNA sequences interspersed between protein-coding DNA sequences called exons. When a gene is transcribed, introns are removed by the splicing machinery to generate a spliced messenger RNA (mRNA)1,2. Introns can affect gene expression in several ways (reviewed in3). For example, introns facilitate creation of different protein isoforms – a phenomenon known as alternative splicing4,5. Introns can also encode catalytic and regulatory RNAs6,7,8,9,10,11. And, introns may restrict genomic instability12. Finally, and the focus of this report, introns can increase the expression level of a gene via a number of different mechanisms. The expression level increase is referred to as intron mediated enhancement of gene expression level (IME; reviewed in13,14,15).

In general, IME is defined as the increase in expression of an intron-containing gene compared to an otherwise-identical intronless version of the same gene16. There are several mechanisms by which introns can increase expression levls13,14,15. In mammalian and plant systems, introns increase the rate of transcription, and this can be mediated by splicing independent functions of splicing factors such as U1 snRNA17,18,19 or by gene looping20. Moreover, proteins deposited onto spliced transcripts, called the exon junction complex (EJC), increase mRNA transport from the nucleus to the cytoplasm21,22. The EJC can also increase translation efficiency of spliced transcripts23,24,25. Experiments swapping the sequences and orientations of introns suggest the proximity of the intron to the promoter is critical for IME26, and not the orientation of the sequence. Rose and Gallegos created a model wherein promoter-proximal introns contain stimulatory signals controlling transcription initiation or re-initiation, in a chromatin state dependent manner, either allowing an open chromatin state, or facilitating activating histone modifications14.

A 5′-intron near the promoter is often sufficient for IME27,28. 5′-introns can increase gene expression levels by increasing recruitment of RNA polymerase II to the transcription initiation site18,29. Promoter-proximal introns can also affect transcription initiation via chromatin modifications30,31. A 5′-intron is sufficient to recruit chromatin opening marks30. Previous reports measured 5′-intron IME in terms of RNA abundance27,30,32, protein activity27,28 or phenotype (related directly to protein activity33). The contemporaneous best available technologies for these studies had technical caveats like the inability to control transgene copy number and locus (either of which could have affected transgene expression level34,35). The in vitro measurements of IME at the RNA and protein level were assayed via biochemical extracts27,28. Finally, in some cases, reporter gene expression was transient23 (temporally unstable due to the nature of transfection/transduction). With modern genome editing and microfluidic technologies, we are able to quantify IME in live C. elegans with improved technical precision.

Below we describe a microfluidic imaging system we developed to study IME in vivo in C. elegans. We designed a system that is higher throughput than confocal microscopy but still image based (unlike worm-flow)34, and compatible with fluorescent stereoscopes found in most C. elegans labs. We show the effects of different sequences and positions of natural and synthetic introns on the expression level of mCherry, controlled by the hsp-90 promoter. We also show the effects of a different promoter (vit-2) and a different coding sequence (mEGFP) on IME. We compare the size distribution of introns using modern drafts of the C. elegans and human genomes. Understanding details of IME is important for basic biology36, biotechnology37,38, and human diseases. There are now several diseases in which mutations in introns affect gene regulation39,40. Utilizing C. elegans to understand the biology of introns in vivo may offer complementary, novel physiological or molecular insights.


A cost-effective, semi-automated microfluidic device for quantifying gene expression in individual animals

We modified a previous microfluidic chip design41,42 to develop an instrument to quantify gene expression in whole animals while acquiring an image of each animal. In this system, air pressure moves the worms from a pressurized 1.5 mL tube into the PDMS chip where they are subsequently imaged (Fig. 1). On the chip, animals are imaged in a “U” shaped orientation, and then flow out of the imaging chamber into an exit tube. The design can accommodate manual sorting of animals with complex phenotypes by diverting the exit tube onto solid or liquid worm growth media. Figure 1a shows an overview of the setup. Figure 1b details the process of entry and exit into the imaging chamber. Figure 1c shows how we quantify signal from animals. We can typically image about 100 worms in twenty minutes. This instrument is not as fast as the Copas Biosort, but it captures an image of every worm. For our initial experiments with hsp-90 reporters, we used exposure and excitation near the bottom of the linear dynamic range (Supplementary Fig. 1) to ensure the same settings could be used with stronger promoters/signals. We validated the dynamic range of the system using dilutions of lyophilized mCherry; the dynamic range covers a more than 50 fold range of protein concentration (Supplementary Fig. 1). We validated the microfluidic system’s results using a COPAS Biosort (worms measured in flow) and quantitative, cell-resolution confocal microscopy34 (see below and Supplementary Fig. 1).

Figure 1

Overview of microfluidic imaging device schematics and image calibration procedure. (a) shows a schematic overview of the worm microfluidic measurement device. (b) displays a time series of cartoons showing valve openings and closings occurring during imaging of experimental groups of worms. Depth of field on our objective is approximately 55 micrometers (see Materials and Methods), and our imaging chamber is 50 micrometers deeps in z, ensuring we capture all the signal form each animal. (c) shows the image correction protocol we used to determine average voxel intensity, quantifying expression level as a function of concentration (not total signal); see Supplementary Fig. 1 for an image of a fluorescent worm in the imaging chamber.

A set of C. elegans expressing fluorescent proteins with different intron configurations

We designed and constructed a set of eleven different C. elegans strains with single copy reporter genes at a Chromosome II locus, designated by a transposon insertion, ttTi560535,43,44,45. We varied the promoter (hsp-90 or vit-2), and/or number, position or sequence of introns (synthetic or natural); we used the unc-54 terminator in all constructs. The constructs integrated into the genomes of the resulting strains are shown in Fig. 2 and listed in Table 1. The sequences of all reporter gene are listed in Supplementary Information. Because autofluorescence levels are significantly lower in mCherry, relative to mEGFP, we used mCherry for the majority of the work, except when testing the effect protein coding sequence on IME.

Figure 2

Characterization of intron sequence and location on IME from a set of transgenic C. elegans strains expressing fluorescent reporter proteins with and without introns from a single autosomal locus in the genome. Error bars show 95% confidence intervals. Differences between strains are therefore at a 5% significance level when the error bars do not overlap. (*) indicates strains that have a statistically significant increase relative to the no-intron containing control. Additional statistical details are provided in materials and methods and Supplementary Information. (a) Strains using the hsp-90 promoter driving mCherry coding sequences that contain variable numbers and locations of introns. The exons are shown in red, and introns are shown in yellow or blue, and labeled ia-e. Introns ia-c are the canonical synthetic introns, and id-e are the first two introns naturally found in C. elegans’ hsp-90. Regardless of intron sequence, or number, all strains received a ~50% boost in gene expression as long as a 5′-intron was present. A single 3′-intron was insufficient to boost expression relative to a transgene with no introns. (b) The protein coding sequence affects the magnitude of IME. Using strains expressing EGFP and containing either no introns, or three synthetic introns, an ~80% boost was quantified. (c) IME is not constrained by free translation capacity, as mCherry expressed by one of the strongest promoters also received ~50% expression boost from three synthetic introns. (d) There is a slight but significant increase of IME effect size when mCherry expression is controlled by the much stronger vit-2 promoter (53.55% vs. 45.88%). (e) Analysis of the intron lengths in both C. elegans and humans.

Table 1 .

The canonical three synthetic introns increase mCherry expression by approximately 50%

Because of the widespread usage of the three canonical synthetic introns created by Dr. Andrew Fire46, we first determined the magnitude of IME using these introns. We used the constitutive, ubiquitously expressed, hsp-90 promoter to control expression of mCherry with and without these synthetic introns. We expressed these reporter genes from the same locus on chromosome II (inserted at ttTi560535,44,45). By measuring average voxel mCherry signal in our microfluidic chip (after correcting for intrinsic fluorescence in wild-type animals), we found the canonical three synthetic introns increase mCherry expression level by 46% (Fig. 2a). We confirmed our findings using two additional instruments. We found the same IME effect size (Supplementary Fig. 1) at cell resolution using a confocal microscope34. We also saw a similar IME effect at animal resolution (Supplementary Fig. 1) using the COPAS Biosort34.

Two natural introns have the same IME magnitude as three synthetic introns

The canonical introns used in C. elegans reporter transgenes are synthetic, so we determined if natural introns confer IME. We replaced the synthetic introns in our Phsp-90::mCherry::Tunc-54 construct with the first two natural hsp-90 introns. We placed the natural introns at the same exon-intron boundaries as the synthetic introns, maintaining the same 5′- and 3′- splice junctions (Fig. 2). The natural hsp-90 introns increased protein expression the same as the three synthetic introns (~50%), shown in Fig. 2a.

A single 5′-intron is sufficient for IME

Prior reports demonstrated a single 5′-intron is sufficient for some level of IME27,28,33. Therefore, we tested if single 5′-introns are sufficient for IME. We generated strains containing one, two or three synthetic or natural introns at different positions in the coding sequence, shown in Fig. 2a. We found that two synthetic introns produced the same IME effect as all three synthetic introns. Strains containing only a single natural or synthetic intron at the first position had the same level of IME as all three synthetic introns or both natural introns (Fig. 2a). A single intron at the 3′-site was not sufficient to increase gene expression, at least when intron “a” was used (this intron is sufficient for IME when placed 5′ (Fig. 2a)).

Protein coding sequence affects IME magnitude

To determine if protein coding sequence affects IME, we generated strains containing the mEGFP coding sequence with and without the same three synthetic introns described above. Figure 2b shows that we observed an IME effect size of ~80%, which is significantly different from the ~50% increase in gene expression conferred by the same introns in the context of the mCherry coding sequence. To validate that IME magnitude could be dependent on the coding sequence, we measured animals on four separate days using a point scanning confocal microscope as previously described34. We compared IME for mEGFP and mCherry measured from the same cell (Int1DL) from ~40 animals. Using this approach, we again found coding sequence affected IME (Supplementary Fig. 1c). While there are certainly more coding sequences to test, these results suggest that coding sequence can impact IME, at least for two divergent fluorescent proteins (48% and 30% identity at the DNA and protein level respectively).

IME magnitude is slightly affected by the vit-2 promoter

To determine if promoter strength affects IME, we replaced the hsp-90 promoter with vit-2. The vit-2 gene encodes a yolk protein expressed in the intestine of C. elegans. When considering it is only expressed from the 20 intestine cells47, it is possibly the most highly expressed gene (in terms of concentration) in adulthood48, which is when we measured the animals (day one of adulthood). If IME were primarily constrained by free translational capacity, the magnitude of protein increase could depend on the transcriptional strength of the promoter. Stronger promoters would thus receive less of a boost. We constructed two strains using the vit-2 promoter to drive mCherry with either no introns, or the three synthetic introns (Fig. 2c; Table 1). The presence of three synthetic introns increased protein expression by ~50% (Fig. 2c) showing that the distinctly regulated, and stronger vit-2 promoter does not have a large effect on the magnitude of IME at the protein level compared to the hsp-90 promoter (Fig. 2d). Note the absolute value of protein produced under vit-2 promoter control was much higher. Thus, the magnitude of IME does not appear to be constrained by free translational capacity. Most promoters remain untested, and may confer larger or smaller IME effect sizes.

Intron size distributions in C. elegans and H. sapiens

Relatively short, single 5′-introns are sufficient to increase gene expression level in plants27,32 and mammals28, and now we can add C. elegans to the list. The short single introns in these studies are generally representative of short introns sizes in worms and humans. Figure 2E shows the distribution of intron sizes in modern drafts of the C. elegans and H sapiens genomes. Both worms and humans have ~20,000 protein coding genes, but the human genome is about ten times bigger. Introns in C. elegans are generally shorter than in humans, and both C. elegans and H. sapiens have a modal cluster of short introns (Fig. 2E). However, perhaps as expected with a more compact genome, short introns compose a larger proportion of total introns in C. elegans.


The microfluidic quantification system described here allowed us to quantify whole animal reporter gene signals from live C. elegans at medium throughput (30–100 animals in 30 minutes). Our previous measurements of gene expression in whole animals34,49,50,51 utilized a higher throughput (300–1000 animals in similar timeframe) flow sorting device (COPAS Biosorter, Union Biometrica) that measures signal from animals as they pass by a photomultiplier tube. This instrument does not acquire images of each animal, forgoing acquisition of potentially useful image features. By contrast, our system collects an image of each animal in a fixed orientation (see Fig. S1). Our particular system’s objective lens has a large depth of field, (>50 µm) allowing collection of signal from the entire depth in z of the immobilized worm.

In this report, we found that single or multiple introns increased protein expression level by about 50% or 80%, depending on coding sequence, and these data fit within the previously reported range of IME. In Nott et al., 2003, a single 5′-intron was sufficient to increase gene expression by 29-fold28. In Bieberstein et al. 2012, the mouse FOS gene expressed five times more RNA when it included three natural mouse introns; they also found a single 5′-intron sufficient to cause chromatin markings associated with increased gene expression level30. In Rose 2002, several distinct Arabidopsis introns were identified, which increased expression of reporter gene RNA between 40% and 1490%32. Similarly, in Rose 2004, a variety of single 5′-proximal Arabidopsis introns were found to be sufficient for IME, ranging from 10% to 2000%27. Thus, the effects we measured fall between the effects that have been measured before. Most introns remain untested in C. elegans (see additional examples and scenarios in52). It is possible that future studies will identify C. elegans intron sequences that confer greater IME effect sizes than we found here.

Our findings corroborate results from prior in vitro studies showing that a single 5′-intron is sufficient for IME using phenotypes33, extracted RNA27 or extracted protein activity27,28 as readouts for the effect of introns on gene expression level. Our results are consistent with the idea that IME occurs via transcription, translation, or both. Our results are consistent with a transcriptional mechanism in which the splicing machinery at the 5′-intron re-recruits RNA polymerase back to the transcriptional start site. Our results are consistent with the possibility that a 5′-intron may affect transcription by opening chromatin; this may occur via recruitment of molecular machinery that makes access-permissive histone modifications to promoter-proximal histones. Our results are also consistent with the possibility that a 5′-intron can affect translation via proteins deposited onto the EJC during splicing53,54, which can recruit the translation machinery to EJC-marked transcripts55. Clearly, more work is needed to answer many lingering questions about IME13,14,15,56.

Many previous reports have shown that introns influence human diseases. These diseases fall into two broad classes: heritable diseases39,40,57 and cancers58,59,60,61,62. The intron-dependent processes affected are often unknown, poorly understood, or if understood, lack experimental means to determine how to suppress the intron-related pathology. For example, in a bipolar risk allele, STRs in the third intron of the calcium encoding CACNA1C gene seem to affect IME40. However, the mechanism by which this large, relatively 3′-intron acts to increase gene expression level remains unknown. The reagents and tools described here will allow investigators to perform genetic screens for factors affecting intron-related phenotypes. Moreover, the recent development of synthetic food medium as an alternative to the OP50 E. coli strain should allow execution of chemical genetic screens, using, for example, FDA approved pharmaceuticals packaged into liposomes and delivered into the intestine cells via the alimentary canal63. It is our hope that the screenable in vivo system we presented here will provide insight into intron-related disease mechanisms, and lead to therapeutic treatments to alleviate human diseases.

Materials and Methods

Strains and culture conditions

Here, we used wild-type N2 strain of C. elegans, RBW6699 and the strains we listed in Fig. 1 and Table 1: RBW2642, RBW2661, RBW2511, RBW2581, RBW2631, RBW2651, RBW2721, RBW2741, RBW2731, RBW2761, RBW2711, and RBW2751; each strain has an insert number corresponding to its strain number, meaning RBW2511 contains hutSi2511. We maintained all strains in 10 cm petri dishes on NGM seeded with OP50 E. coli in an incubator at 20°. Strains were maintained for at least three generations at 20° in a well-fed state before experimentation. For experiments, animals were synchronized via alkaline hypochlorite treatment followed by overnight hatchout in sterile S-basal to cause entry into the L1 diapause, described in detail in34. Synchronous cohorts of animals were grown to gravid adulthood (72 hours at 20° on NGM after the L1 diapause) at a density of approximately 175 animals per 10 cm NGM plate seeded with 1 mL of OP50 E. coli from an overnight 500 mL or 1000 mL LB growth culture.

Generation of transgenic nematodes

To make the DNA used for the transgenes we inserted, we used a yeast homologous recombination based cloning system we described recently to generate DNA repair templates64. To make the reporter genes, we used 2 kb upstream of the ATG for the hsp-90 promoter and 4 kb upstream of the ATG for the vit-2 promoter. We used the same unc-54 terminator in all strains64. For the pieces of DNA in which we varied introns, we created those with overlap extension PCR and then used those fragments in our yeast HR cloning system. These DNA repair templates were then used in microinjection-based MosSCI transgenesis to insert these reporter genes into the genome at the ttTi5605 Mos transposon locus on Chromosome II in strain RBW6699, which is an outcrossed version of EG6699 containing unc-119(ed3). We injected constructs @ 50 ng/µL of repair template + coinjection and selection markers as described previously in35,44,45). We sequenced the reporter sequences of all DNA constructs to ensure their sequence integrity and genotyped animals to verify insertion at the ttTi5605 locus. Thus, in this way, we generated all the strains listed in Fig. 2 and Table 1, with the exception of RBW2642 and RBW2661, which were previously reported in34. We show the sequences of the fluorescent proteins and introns used in Supplementary Information.

Microfluidic measurements of whole-worm gene expression levels

To prepare animals for entry into the microfluidic imaging device, we washed them from their growth plates into 1.5 mL Eppendorf tubes with filter-sterilized S-basal (to remove particulate matter) containing 0.01% tween (to prevent worms from sticking to plastic). We allowed animals to settle for 5–10 minutes in a 50 mL conical vial, and then removed the supernatant, and added fresh S-basal. We repeated this process three times to ensure that that any dust or embryos washed from the plates were removed prior to use. We then placed a “slurper” tube into the 1.5 mL Eppendorf tube. After we sealed and pressurized the Eppendorf tube and slurper lid, animals flowed into the slurper tube, and we manually loaded animals, one at a time, into the imaging chamber of the microfluidic device. We measured all strains, one worm at a time. To quantify signal from the animals in microfluidic device, we mounted the device on a wooden jig under a LED-illuminated, fluorescent Leica MFC165 stereoscope (Leica Microsystems, Wetzlar, Germany) set to 12X magnification, equipped with a 1.0x Planapo objective and a Lumenera 3–3UR camera with a 0.63X optical coupler, in a room at approximately 20°. With this setup our depth of field was approximately 55 micrometers and our xy resolution was approximately 2.7 micrometers. We focused the image on the approximate central plane of the worm body by focusing on the grinder and lumen of the pharynx, shown in Fig. 1 in65. Worms were physically constrained by the 65 × 50 micrometer dimensions of the imaging chamber preventing them from moving their bodies out of plane. We measured between 30 and over 100 animals per strain per experiment. We manually controlled the flow into the imaging and image acquisition in real time on the computer using Matlab and Micromanager, respectively.

Microfluidic worm measurement device construction

Devices are constructed as previously detailed in41,42, using standard soft-lithography practices. Briefly, a microfluidic master mold was constructed in the University of Washington Nanofabrication facility using a transparency mask from OutputCity. The AutoCAD® design files are available at GitHub at The mold was made using SU8 2025 and following fabrication was coated with a monolayer of silane to prevent PDMS adhesion. The individual microfluidic devices were constructed using a two layer fabrication process similar to42 wherein a very small amount high pre-polymer to cross-linker (20:1) ratio of Sylgard 184 was poured on the microfluidic mold sufficient to cover the mold with 1–4 mm of polymer. This 20:1 ratio has a very low Young’s Modulus which allows the side valve mechanism to work. This base layer is partially cured for 45 minutes in a 70 °C oven, and then a volume of 10:1 (pre-polymer:cross-linker) Sylgard 184 is poured on top. Because the low Young’s Modulus layer is prone to damage or tearing due to the limited polymer cross-linking, this second layer provides much needed structural support for inlet hole punching and bonding. This stability layer is poured to a thickness of 15–20 mm.

Following curing of the stability layer (2 + hours in 70 °C oven), devices are cut and removed from the mold. Holes for media and valves were punched using a 1 mm diameter biopsy punch. To ensure that PDMS debris was not introduced to the flow channels as a result of the hole punching, the quality of the biopsy punch was inspected prior to use, and holes were only punched cleanly once. All holes were punched from the device side through the low Young’s Modulus flow layer. Devices were cleaned using scotch tape prior to bonding to slide glass. Bonding was performed using a Harrick PDC-001 plasma cleaner. Devices and slide glass were exposed to high energy plasma for 35 seconds, and then the device was placed on the slide glass. Bonded devices were allowed to rest for at least 1 h in the 70 °C oven prior to use.

To prevent diffusion of gas from the pressurized valves into the flow channels, valves were filled with water prior to use. This was done by connecting tubes containing DI water to the valve channels, and pressurizing the channels. The flushing media for the device used to push out the worms uses filtered M9 media containing 0.01% Triton X-100. To ensure that there was no cross-contamination between samples, the flushing media was used at moderate pressure for 5 min to rinse the device and the loading tubes. Loading and flushing pressures were optimized for each experimental run, but were typically within the range of 1–9 psi. The valve pressures were similarly optimized for individual variation in devices, but typically were used in the range of 45–60 psi.

Valve controller box fabrication

To control both the valve pressures, and whether the valves are pressurized or released, we developed a control box composed of pressure regulators, solenoid valves, and a USB switch to provide computerized control of all on-chip valves. This system was modeled on earlier systems41,42,66. A complete list of parts is available on GitHub at

Valve control software

Computerized control of both the microfluidic valve control box and the microscope camera was implemented using custom written Matlab® software. To provide generalizability, all interaction with microscope and camera was implemented using micromanager, and controlled through Matlab®. The code, is freely available on GitHub at

Quantification of whole animal fluorescence

Image segmentation was automated to ensure consistency in quantification of expression level between different strains. Images from all strains for the same promoter, fluorescent protein combination were loaded into memory, and an appropriate segmentation threshold that minimized intraclass variance was selected using Otsu’s threshold67. Following subtraction of the camera noise from image, all animals containing transgenes with the same promoter/fluorescent protein combination were segmented using the same threshold. Segmented images were then opened to remove any spurious pixels. This whole animal segmentation provided consistent size measurements between strains, and had a high degree of accuracy when compared with manual segmentation of the brightfield images.

Microscope corrections and calibrations

There are multiple sources of noise and variation that are generated during conventional epifluorescent imaging. To ensure we operated in the linear range of our camera, we quantified a mCherry dilution series we generated from lyophilized mCherry, shown in Supplementary Fig. 1. To correct for individual pixel variation in dark current noise in the CCD camera, we acquired 1,000 images using the same exposure times and binning, but without light directed to the camera. For each pixel, the median value was determined and used as the dark current pixel noise, and was subtracted from each image for all experiments. To correct for intrinsic autofluorescence, we imaged approximately 100 wild-type N2 animals using the two image acquisition settings (excitation/emission filters and camera exposure time) we used to image animals expressing mCherry or mEGFP. The whole animal auto-fluorescence was determined using both the mEGFP and mCherry parameters. There is a distribution of autofluorescence levels within the wild-type population, but for simplicity the population mean autofluorescence levels for both the mEGFP and mCherry fluorescent excitation/emission channels were calculated. These mean values were then subtracted from the transgene expressing strains to determine the magnitude of fluorescence from the fluorescent protein.

For those who care to undertake these endeavors comparing mCherry and mEGFP, we note that the intrinsic fluorescence signals emanating from secondary lysosomes and other intrinsically fluorescent (autofluorescent) bio molecules in wild-type N2 animals34,68 are relatively stronger when excited and imaged with mEGFP optics (and thus a greater part of the total signal measured under those optics), compared to the same images acquired using an mCherry filter set. Because of this increased background effect, to accurately determine the level of fluorescence derived from the GFP transgene, it is critical to calculate and then remove the level of autofluorescence from the imaged cells69 (or circumvent it by measuring an area with relatively pure reporter signal34).

Cell-specific, quantitative confocal microscopy

We performed microscopy as in34, except that the animals were mounted in a microfluidic imaging chamber, described in64. Briefly, in four independent experiments we loaded animals expressing mEGFP or mCherry with or without introns, under control of the hsp-90 promoter, into 80-lane microfluidic mounting devices. We imaged the int1DL cell in ten animals per experiment for animals expressing reporter genes with and without introns in four independent experiments. We quantified expression level of reporters using the equatorial nuclear z slice from the image stacks of each animal, described in34, shown in Supplementary Fig. 1.

Measurement of worms in flow

We performed measurement of worms in flow as previously described in34. Briefly, we synchronized three large populations of worms as described above, and placed the starved L1s onto five 10-cm OP50-seeded NGM plates @ 175 larvae per plate. Then, after 72 hours of development, for each strain (wild-type control, RBW2631 and RBW2642), we used S-basal buffer to wash the adult animals off of the plates and into 15 mL conical vials and allowed the adults to sediment for five minutes, and then aspirated the L1-rich supernatant (the progeny of the adults) three times. We then placed these adult-enriched solutions of worms into the COPAS Biosort and measured fluorescent intensity using mCherry detection optics. We collected the data as a text file and then plotted and analyzed it using SigmaPlot 12.5 (San Jose, CA), shown in Supplementary Fig. 1.

Bioinformatic analysis of intron sizes in protein coding genes in H. sapiens and C. elegans

To collect our data, we used GTF file for Caenorhabditis elegans (WBcel235 Ensembl release 94) and the human GRCh38 (Gencode v29 release GTF file). We used the Bioconductor GenomicRanges package ( to extract intron sizes in protein coding genes in C. elegans and H. sapiens. We plotted the log10 transformed intron size distribution for both C. elegans and H. sapiens using violin plots overlapped with boxplots, shown in Fig. 2.

Statistical methods

We used SigmaPlot 12.5 (San Jose, CA) for all statistical analyses. We performed at least three independent experiments (different batches of animals measured on different days) for each of the strains we measured on our microfluidic device. To analyze the data, we first determined if the data was normally distributed and had equal variance using one or two-way ANOVA, and then performed appropriate statistical tests to account for multiple comparisons. We detail the major analyses used in this paper below.

The specific statistical tests and results for the microfluidic measurements are as follows: Three synthetic introns increase expression level by 45.88%; P < 0.001, Mann-Whitney Rank Sum Test. Given that we measured several different strains compared to the control, we also performed a Kruskal-Wallis One Way Analysis of Variance on Ranks followed by Dunn’s Method to compare to the intronless control and detected a significant difference P < 0.05. At least three independent experiments with at least 185 animal were performed (185–297). Natural introns significantly increase the expression level of mCherry by 47.98%; P < 0.001, Mann-Whitney Rank Sum Test. Given that we measured several different strains compared to the control, we also performed a Kruskal-Wallis One Way Analysis of Variance on Ranks followed by Dunn’s Method to compare to the intronless control and detected a significant difference P < 0.05. We performed at least three experiments with at least 179 total animals were performed (179–297 animals). With the exception of animals bearing the reporter gene with only ia at the most 3′ position in the coding sequence, all other intron bearing strains expressed significantly more protein than the intronless strain; Kruskal-Wallis One Way Analysis of Variance on Ranks followed by Dunn’s Method, P < 0.05 for all comparisons. We performed at least three independent experiments with at least 100 total worms per strain (103–298 animals per group). Synthetic introns significantly increase mCherry expression level under vit-2 control. Mann-Whitney Rank Sum Test, P < 0.001. Results are from three independent experiments with at least 130 total animals per group (134–163). The same three synthetic introns in mCherry significantly increase mEGFP expression level by 83%. P < 0.001, Two Way ANOVA followed by the Holm-Sidak method for multiple comparison procedures. Results are from three independent experiments measuring at least 200 total animals per group (215–260). Details for each of the statistical analyses performed in SigmaPlot are included in Supplementary Information.

Data Availability

Expression level data is available in upon request. Strains will be sent to the CGC. Software and parts lists are available online at github.


  1. 1.

    Shi, Y. Mechanistic insights into precursor messenger RNA splicing by the spliceosome. Nature reviews. Molecular cell biology 18, 655–670, (2017).

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Shi, Y. The Spliceosome: A Protein-Directed Metalloribozyme. Journal of molecular biology 429, 2640–2653, (2017).

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Jo, B. S. & Choi, S. S. Introns: The Functional Benefits of Introns in Genomes. Genomics Inform 13, 112–118, (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Roy, B., Haupt, L. M. & Griffiths, L. R. Review: Alternative Splicing (AS) of Genes As An Approach for Generating Protein Complexity. Current genomics 14, 182–194, (2013).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Carazo, F., Romero, J. P. & Rubio, A. Upstream analysis of alternative splicing: a review of computational approaches to predict context-dependent splicing factors. Brief Bioinform. (2018).

    Article  PubMed  Google Scholar 

  6. 6.

    Wong, J. J., Au, A. Y., Ritchie, W. & Rasko, J. E. Intron retention in mRNA: No longer nonsense: Known and putative roles of intron retention in normal and disease biology. BioEssays: news and reviews in molecular, cellular and developmental biology 38, 41–49, (2016).

    Article  Google Scholar 

  7. 7.

    Hube, F., Ulveling, D., Sureau, A., Forveille, S. & Francastel, C. Short intron-derived ncRNAs. Nucleic acids research 45, 4768–4781, (2017).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  8. 8.

    Bai, Y. et al. Identification of genome-wide non-canonical spliced regions and analysis of biological functions for spliced sequences using Read-Split-Fly. BMC Bioinformatics 18, 382, (2017).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Steiman-Shimony, A., Shtrikman, O. & Margalit, H. Assessing the functional association of intronic miRNAs with their host genes. Rna, (2018).

    CAS  Article  Google Scholar 

  10. 10.

    Joung, J. et al. Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood. Nature 548, 343–346, (2017).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Engreitz, J. M. et al. Local regulation of gene expression by lncRNA promoters, transcription and splicing. Nature 539, 452–455, (2016).

    ADS  CAS  Article  PubMed  Google Scholar 

  12. 12.

    Bonnet, A. et al. Introns Protect Eukaryotic Genomes from Transcription-Associated Genetic Instability. Molecular cell 67, 608–621 e606, (2017).

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Shaul, O. How introns enhance gene expression. Int J Biochem Cell Biol, (2017).

    CAS  Article  Google Scholar 

  14. 14.

    Gallegos, J. E. & Rose, A. B. The enduring mystery of intron-mediated enhancement. Plant science: an international journal of experimental plant biology 237, 8–15, (2015).

    CAS  Article  Google Scholar 

  15. 15.

    Le Hir, H., Nott, A. & Moore, M. J. How introns influence and enhance eukaryotic gene expression. Trends in biochemical sciences 28, 215–220, (2003).

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Brinster, R. L., Allen, J. M., Behringer, R. R., Gelinas, R. E. & Palmiter, R. D. Introns increase transcriptional efficiency in transgenic mice. Proceedings of the National Academy of Sciences of the United States of America 85, 836–840 (1988).

    ADS  CAS  Article  Google Scholar 

  17. 17.

    Kwek, K. Y. et al. U1 snRNA associates with TFIIH and regulates transcriptional initiation. Nat Struct Biol 9, 800–805, (2002).

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Damgaard, C. K. et al. A 5′ splice site enhances the recruitment of basal transcription initiation factors in vivo. Molecular cell 29, 271–278, (2008).

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Furger, A., O’Sullivan, J. M., Binnie, A., Lee, B. A. & Proudfoot, N. J. Promoter proximal splice sites enhance transcription. Genes & development 16, 2792–2799, (2002).

    CAS  Article  Google Scholar 

  20. 20.

    Moabbi, A. M., Agarwal, N., El Kaderi, B. & Ansari, A. Role for gene looping in intron-mediated enhancement of transcription. Proceedings of the National Academy of Sciences of the United States of America 109, 8505–8510, (2012).

    ADS  Article  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Le Hir, H., Gatfield, D., Braun, I. C., Forler, D. & Izaurralde, E. The protein Mago provides a link between splicing and mRNA localization. EMBO reports 2, 1119–1124, (2001).

    Article  PubMed  PubMed Central  Google Scholar 

  22. 22.

    Valencia, P., Dias, A. P. & Reed, R. Splicing promotes rapid and efficient mRNA export in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America 105, 3386–3391, (2008).

    ADS  Article  PubMed  PubMed Central  Google Scholar 

  23. 23.

    Nott, A., Le Hir, H. & Moore, M. J. Splicing enhances translation in mammalian cells: an additional function of the exon junction complex. Genes & development 18, 210–222, (2004).

    CAS  Article  Google Scholar 

  24. 24.

    Lee, H. C., Choe, J., Chi, S. G. & Kim, Y. K. Exon junction complex enhances translation of spliced mRNAs at multiple steps. Biochem Biophys Res Commun 384, 334–340, (2009).

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Wiegand, H. L., Lu, S. & Cullen, B. R. Exon junction complexes mediate the enhancing effect of splicing on mRNA expression. Proceedings of the National Academy of Sciences of the United States of America 100, 11327–11332, (2003).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  26. 26.

    Rose, A. B., Emami, S., Bradnam, K. & Korf, I. Evidence for a DNA-Based Mechanism of Intron-Mediated Enhancement. Frontiers in plant science 2, 98, (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  27. 27.

    Rose, A. B. The effect of intron location on intron-mediated enhancement of gene expression in Arabidopsis. The Plant journal: for cell and molecular biology 40, 744–751, (2004).

    CAS  Article  Google Scholar 

  28. 28.

    Nott, A., Meislin, S. H. & Moore, M. J. A quantitative analysis of intron effects on mammalian gene expression. Rna 9, 607–617 (2003).

    CAS  Article  Google Scholar 

  29. 29.

    Das, R. et al. SR proteins function in coupling RNAP II transcription to pre-mRNA splicing. Molecular cell 26, 867–881, (2007).

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    Bieberstein, N. I., Carrillo Oesterreich, F., Straube, K. & Neugebauer, K. M. First exon length controls active chromatin signatures and transcription. Cell Rep 2, 62–68, (2012).

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    Parra, G., Bradnam, K., Rose, A. B. & Korf, I. Comparative and functional analysis of intron-mediated enhancement signals reveals conserved features among plants. Nucleic acids research 39, 5328–5337, (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  32. 32.

    Rose, A. B. Requirements for intron-mediated enhancement of gene expression in Arabidopsis. Rna 8, 1444–1453 (2002).

    CAS  Article  Google Scholar 

  33. 33.

    Okkema, P. G., Harrison, S. W., Plunger, V., Aryana, A. & Fire, A. Sequence requirements for myosin gene expression and regulation in Caenorhabditis elegans. Genetics 135, 385–404 (1993).

    CAS  PubMed  PubMed Central  Google Scholar 

  34. 34.

    Mendenhall, A. R., Tedesco, P. M., Sands, B., Johnson, T. E. & Brent, R. Single Cell Quantification of Reporter Gene Expression in Live Adult Caenorhabditis elegans Reveals Reproducible Cell-Specific Expression Patterns and Underlying Biological Variation. PloS one 10, e0124289, (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  35. 35.

    Frokjaer-Jensen, C. et al. Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Nature methods 11, 529–534, (2014).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  36. 36.

    Yofe, I. et al. Accurate, model-based tuning of synthetic gene expression using introns in S. cerevisiae. PLoS Genet 10, e1004407, (2014).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  37. 37.

    Lacy-Hulbert, A. et al. Interruption of coding sequences by heterologous introns can enhance the functional expression of recombinant genes. Gene therapy 8, 649–653, (2001).

    CAS  Article  PubMed  Google Scholar 

  38. 38.

    Xu, D. H. et al. SV40 intron, a potent strong intron element that effectively increases transgene expression in transfected Chinese hamster ovary cells. J Cell Mol Med 22, 2231–2239, (2018).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  39. 39.

    Vaz-Drago, R., Custodio, N. & Carmo-Fonseca, M. Deep intronic mutations and human disease. Hum Genet 136, 1093–1111, (2017).

    CAS  Article  PubMed  Google Scholar 

  40. 40.

    Riley, D. E. & Krieger, J. N. Short tandem repeat (STR) replacements in UTRs and introns suggest an important role for certain STRs in gene expression and disease. Gene 344, 203–211, (2005).

    CAS  Article  PubMed  Google Scholar 

  41. 41.

    Crane, M. M. et al. Autonomous screening of C. elegans identifies genes implicated in synaptogenesis. Nature methods 9, 977–980, (2012).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  42. 42.

    San-Miguel, A. et al. Deep phenotyping unveils hidden traits and genetic relations in subtle mutants. Nat Commun 7, 12990, (2016).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  43. 43.

    Frokjaer-Jensen, C. Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9. Genetics 195, 635–642, (2013).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  44. 44.

    Frokjaer-Jensen, C., Davis, M. W., Ailion, M. & Jorgensen, E. M. Improved Mos1-mediated transgenesis in C. elegans. Nature methods 9, 117–118, (2012).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  45. 45.

    Frokjaer-Jensen, C. et al. Single-copy insertion of transgenes in Caenorhabditis elegans. Nature genetics 40, 1375–1383 (2008).

    CAS  Article  Google Scholar 

  46. 46.

    Fire, A. Fire Lab C. Elegans Vector Kit 1995 (Stanford Universit School of Medicine, 1995).

  47. 47.

    Spieth, J., MacMorris, M., Broverman, S., Greenspoon, S. & Blumenthal, T. Regulated expression of a vitellogenin fusion gene in transgenic nematodes. Developmental biology 130, 285–293 (1988).

    CAS  Article  Google Scholar 

  48. 48.

    Hillier, L. W. et al. Massively parallel sequencing of the polyadenylated transcriptome of C. elegans. Genome Res 19, 657–666 (2009).

    CAS  Article  Google Scholar 

  49. 49.

    Mendenhall, A. et al. Environmental Canalization of Life Span and Gene Expression in Caenorhabditis elegans. The journals of gerontology, (2017).

    CAS  Article  Google Scholar 

  50. 50.

    Mendenhall, A., Crane, M. M., Tedesco, P. M., Johnson, T. E. & Brent, R. Caenorhabditis elegans Genes Affecting Interindividual Variation in Life-span Biomarker Gene Expression. The journals of gerontology, (2017).

    CAS  Article  Google Scholar 

  51. 51.

    Mendenhall, A. R. et al. Expression of a single-copy hsp-16.2 reporter predicts life span. The journals of gerontology 67, 726–733 (2012).

    Article  Google Scholar 

  52. 52.

    Frokjaer-Jensen, C. et al. An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline. Cell 166, 343–357, (2016).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  53. 53.

    Kotovic, K. M., Lockshon, D., Boric, L. & Neugebauer, K. M. Cotranscriptional recruitment of the U1 snRNP to intron-containing genes in yeast. Molecular and cellular biology 23, 5768–5779 (2003).

    CAS  Article  Google Scholar 

  54. 54.

    Listerman, I., Sapra, A. K. & Neugebauer, K. M. Cotranscriptional coupling of splicing factor recruitment and precursor messenger RNA splicing in mammalian cells. Nature structural & molecular biology 13, 815–822, (2006).

    CAS  Article  Google Scholar 

  55. 55.

    Cheng, H. et al. Human mRNA export machinery recruited to the 5′ end of mRNA. Cell 127, 1389–1400, (2006).

    CAS  Article  PubMed  Google Scholar 

  56. 56.

    Heyn, P., Kalinka, A. T., Tomancak, P. & Neugebauer, K. M. Introns and gene expression: cellular constraints, transcriptional regulation, and evolutionary consequences. Bioessays 37, 148–154, (2015).

    CAS  Article  PubMed  Google Scholar 

  57. 57.

    Swarbrick, M. M. et al. Replication and extension of association between common genetic variants in SIM1 and human adiposity. Obesity (Silver Spring) 19, 2394–2403, (2011).

    CAS  Article  Google Scholar 

  58. 58.

    Neri, F. et al. Intragenic DNA methylation prevents spurious transcription initiation. Nature 543, 72–77, (2017).

    ADS  CAS  Article  PubMed  Google Scholar 

  59. 59.

    Lee, S. H. et al. Widespread intronic polyadenylation inactivates tumour suppressor genes in leukaemia. Nature. (2018).

    Article  PubMed  PubMed Central  Google Scholar 

  60. 60.

    Jung, H. et al. Intron retention is a widespread mechanism of tumor-suppressor inactivation. Nature genetics 47, 1242–1248, (2015).

    CAS  Article  PubMed  Google Scholar 

  61. 61.

    Wiesner, T. et al. Alternative transcription initiation leads to expression of a novel ALK isoform in cancer. Nature 526, 453–457, (2015).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  62. 62.

    Hsu, T. Y. et al. The spliceosome is a therapeutic vulnerability in MYC-driven cancer. Nature 525, 384–388, (2015).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  63. 63.

    Flavel, M. R. et al. Growth of Caenorhabditis elegans in Defined Media Is Dependent on Presence of Particulate Matter. G3 (Bethesda) 8, 567–575, (2018).

    CAS  Article  Google Scholar 

  64. 64.

    Sands, B. et al. A toolkit for DNA assembly, genome engineering and multicolor imaging for C. elegans. Translational Medicine of Aging (2018).

  65. 65.

    Russell, J. C. et al. Electrophysiological measures of aging pharynx function in C. elegans reveal enhanced organ functionality in older, long-lived mutants. The journals of gerontology, (2017).

  66. 66.

    Chung, K., Crane, M. M. & Lu, H. Automated on-chip rapid microscopy, phenotyping and sorting of C. elegans. Nature methods 5, 637–643, (2008).

    CAS  Article  PubMed  Google Scholar 

  67. 67.

    Otsu, N. Threshold Selection Method from Gray-Level Histograms. Ieee T Syst Man Cyb 9, 62–66, (1979).

    Article  Google Scholar 

  68. 68.

    Clokey, G. V. & Jacobson, L. A. The autofluorescent “lipofuscin granules” in the intestinal cells of Caenorhabditis elegans are secondary lysosomes. Mechanisms of ageing and development 35, 79–94 (1986).

    CAS  Article  Google Scholar 

  69. 69.

    Lichten, C. A., White, R., Clark, I. B. & Swain, P. S. Unmixing of fluorescence spectra to resolve quantitative time-series measurements of gene expression in plate readers. BMC Biotechnol 14, 11, (2014).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

Download references


Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). We would like to thank Nikolay Burnaevskiy for helping with the worm flow measurements. We would also like to thank Lu Wang of the Nathan Shock Bioinformatic Core for her bioinformatic analysis of intron sizes in C. elegans and humans. Funding was provided by National Institute on Aging grants R00AG045341 to A.M, P50 AG005136 to MK, and a Pilot Grant from the Nathan Shock Center for Excellence in the Basic Biology of Aging at the University of Washington to AM (NIA Grant P30AG013280 to MK). Training grant T32AG000057 supported MC. Work by AM and BS was also funded by National Institute of General Medical Science grant R01GM97479 to RB. This work was also funded by the National Cancer Institute grant R01CA219460 to A.M.

Author information




A.M., M.C. and B.S. developed the experimental approach. A.M. and B.S. designed and constructed strains in the R.B. lab. M.C. and A.M. designed and constructed the microfluidic device. S.Y., M.C., C.B. and B.J. performed the experiments. M.C., B.S. and A.M. analyzed the data. A.M., R.B. and M.K. provided funding. All authors were involved in manuscript writing.

Corresponding author

Correspondence to Alex Mendenhall.

Ethics declarations

Competing Interests

The authors declare no competing interests.

Additional information

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Crane, M.M., Sands, B., Battaglia, C. et al. In vivo measurements reveal a single 5′-intron is sufficient to increase protein expression level in Caenorhabditis elegans. Sci Rep 9, 9192 (2019).

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing