Figure 7 | Scientific Reports

Figure 7

From: Propionic Acid Induces Gliosis and Neuro-inflammation through Modulation of PTEN/AKT Pathway in Autism Spectrum Disorder

Figure 7

The Bivalent Role of PPA and BA on Neurite Growth In Vitro. Panel A(a–f) depicts double-immunostained representative images of differentiated neurons labeled with Tubulin-IIIβ and DAPI. Images (g,h) are enlarged views of (b,f), respectively. Magnification 25x and scale bar 25 um. Histogram B depict quantitative analysis of neurite outgrowth in (um) under control, PBS (1x), PPA (0.1, 0.5, 1, and 2 mM) and BA (0.1, 0.5, 1, and 2 mM). Histogram C depicts neurite outgrowth measurements in presence or absence of β-HB pre-treatment. Data is represented as Mean+SEM (n = 15 neurites per group) and statistical significance: (*p < 0.0001, F (10, 111) = 53.15) versus controls was obtained using Wilcoxon matched-pairs and One-way ANOVA followed by Tukey post-hoc test.

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