Figure 2 | Scientific Reports

Figure 2

From: A sensitive and simple targeted proteomics approach to quantify transcription factor and membrane proteins of the unfolded protein response pathway in glioblastoma cells

Figure 2

Application of the HR targeted workflow for the quantification of UPR proteins. (A) Final setup and instrument parameter used for quantification. (B) Representative PRM traces of the targeted peptides corresponding to UPR proteins and their fragment ion traces. (C) Calibration curves for an ATF6 assay with different parameter set-ups. Both x and y axis are log 2 scaled (D) Limit of quantification is lowered by high resolution and longer injection times. (E) Measured copy numbers per cell of the analyzed UPR proteins in the glioblastoma cell line LN-308. The y-axis is presented in log10 scale. All measurements were repeated at least 3 times. To calculate the copy numbers/cell, 3 biological replicates were used and the error is presented as standard deviation.

Back to article page