Figure 6 | Scientific Reports

Figure 6

From: Enhancement of laccase activity by pre-incubation with organic solvents

Figure 6

The enhancement effect of organic-solvent pre-incubation on enzyme activity observed by using phenolic substrates. Acetone and ethanol pre-incubation enhanced laccase activity with 2,6-DMP (a) or guaiacol (b) used as phenolic substrates. Purified laccases including DLac, TvLac, and MtLac were pre-incubated with acetone or ethanol for 1 hr before enzyme activity assay. The enzyme activity of each laccase without organic solvent during pre-incubation was defined as 1-fold. The highly significant difference (**) was set at P < 0.001 in Student’s t-test, compared to their Tris buffer control.

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