Sesamin is a furofuran-type lignan that is found abundantly in seeds of Sesamum indicum (sesame) and has been widely accepted as a dietary supplement with positive effects on human health. The biological activity of sesamin in human cells and organs has been analysed extensively, although comparatively few studies show biological functions for sesamin in planta. Herein we screened sesamin-binding proteins (SBP) from sesame seedling extracts using sesamin-immobilized nano-beads. In subsequent peptide mass fingerprinting analyses, we identified a SBP, Steroleosin B, which is one of the membrane proteins found in oil bodies. In addition, pull-down assays and saturation transfer difference-nuclear magnetic resonance (STD-NMR) experiments demonstrated that sesamin binds directly to recombinant Steroleosin B in vitro. Finally, ectopic accumulations of sesamin and Steroleosin B in transgenic Arabidopsis thaliana plants induced severe growth defects including suppression of leaf expansion and root elongation. Collectively, these results indicate that sesamin influences tissue development in the presence of Steroleosin B.
Sesame plants have been cultivated for more than 5,000 years to enable their seeds and seed oils to be consumed for foods, fuels and various other applications. Sesame seeds are known to accumulate sesamin and other related furofuran-class lignans, which are a group of polyphenolic plant metabolites derived originally from phenylalanine. The biosynthetic pathway for furofuran class lignans has been well studied and many related enzymes and genes have been identified1,2,3. Two radical molecules of coniferyl alcohol, produced by the phenylpropanoid biosynthetic pathway and then oxidized by unknown mechanisms, are stereo-selectively conjugated by a dirigent protein to produce pinoresinol, which is a central precursor of most lignans4.
In sesame seeds, sesamin is synthesized from pinoresinol through the sequential formation of two methylenedioxy bridges in reactions catalysed by the P450 monooxygenase CYP81Q15. Then, a portion of sesamin is oxidatively converted into sesamolin and sesaminol, which generally accumulates as sesaminol triglucosides, by the P450 monooxygenase CYP92B146.
Sesamin has various valuable biological properties in mammals, including anti-oxidative, hypocholesterolemic, anti-inflammatory, neuroprotective and immunomodulatory activities and has been used as an ingredient in health-promoting supplements1,2. However, the biological relevance of sesamin to sesame plants themselves remains elusive. For example, sesamin has considerable anti-feedant activities against predators and moderately inhibits the growth of Spilarctia obliqua7. The putative biological roles of sesamin in planta have been associated with seed maturation and/or germination because sesamin content in developing sesame seeds reaches up to 1% (w/v) and decreases drastically upon germination8. Collectively, these reports suggest that the endogenous target proteins of sesamin play key roles in seed maturation and/or germination and reflect the biological roles of sesamin in planta.
Recently, high performance affinity nano-bead technology has enabled the identification of binding proteins for several small molecules of interest9. We therefore used this affinity technique to determine sesamin-binding proteins (SBPs) using the sesamin-immobilized nano-beads (SIB), which led to identification of Steroleosin B10 as a candidate protein. Simultaneous ectopic expression of CYP81Q1 and Steroleosin B was shown to retard growth in Arabidopsis plants, whereas, no effect was observed when they were expressed individually, implying that sesamin influences plant growth in the presence of Steroleosin B.
Identification of Steroleosin B as a sesamin-binding protein
To identify SBPs that are present during sesame seed germination, we performed affinity screening analyses of SBPs using SIB. Initially, we designed and synthesized sesamin derivative 4 (Fig. 1a) as an affinity probe that displays sesamin structures on the surfaces of nano-beads. To this end, sesamin affinity probe 4, which contains a carboxyl group, was covalently fixed onto amine beads at 0.2 and 2.0 mM to prepare low density- (LD) and high density- (HD) SIB, respectively (Fig. 1b). LD or HD-SIB were then incubated with protein extracts from sesame seedlings in the presence or absence of excess free sesamin. The proteins bound to SIB were then analysed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized by silver staining (Fig. 1c). Among the multiple protein bands obtained using HD-SIB, the binding of the band denoted by the arrow was diminished by excess sesamin (Lanes 3 and 4, Fig. 1c), suggesting competitive binding. In-gel digestion and peptide mass fingerprint analysis of corresponding bands identified three proteins from Sesamum indicum (Supplementary Tables S1 and S2). Based on Mascot probability scoring11, Steroleosin B had the highest score among the candidates. Steroleosin B is one of the membrane proteins known to accumulate primarily in the oil bodies of S. indicum and various other plants10 that is thought to be involved in the dehydrogenation of hydroxysterol due to the presence of a sterol-binding subdomain. Although Steroleosin B has been shown to metabolize the mammalian steroids estradiol and corticosterone10, the endogenous substrates and activities of Steroleosin B in planta remain poorly understood. As both sesamin and Steroleosin B highly accumulated in sesame seeds5,10, their direct binding analyses were carried out.
Confirmation of direct interactions between sesamin and Steroleosin B
Pull-down assays of recombinant GST-fused Steroleosin B
Since affinity screening only provides candidates that may bind to SIB, verification of their direct interactions is needed. To verify binding between sesamin and Steroleosin B, we performed pull-down experiments using recombinant GST-fused Steroleosin B10 and SIB. The resulting SDS-PAGE showed that GST-fused Steroleosin B was captured by SIB similar to the affinity screening. By contrast, Steroleosin B was not captured by control beads (acetyl-immobilized) (AIB, Fig. 1b), indicating that GST-fused Steroleosin B binds to SIB via sesamin-displaying moieties (Fig. 1d).
Saturation transfer difference-nuclear magnetic resonance (STD-NMR) analyses of sesamin and recombinant GST-fused Steroleosin B
To confirm the direct interactions between Steroleosin B and sesamin without modification, STD-NMR12 was performed to create epitope maps of ligands binding to proteins. STD-NMR spectra for sesamin and GST-fused Steroleosin B (Fig. 2, Supplementary Fig. S1) at a molar ratio of 60:1 were identified in all assigned proton signals of sesamin, except for H-7 and H-7′, which had a signal that overlapped with that of water. Signal intensities of protons (H-2, 2′, H-5, 5′ and H-6, 6′) in aromatic ring moieties, including those (H-10, 10′) in 1,3-methylenedioxy groups, were higher than those (H-8, 8′ and H-9ab, 9′ab) in furofuran ring moieties. This finding showed that the aromatic ring moieties and not the furofuran rings of sesamin were proximately located in Steroleosin B during binding (Fig. 2a). By contrast, STD-NMR spectra from the 60:1 mixture of sesamin and GST showed limited signals from protons of sesamin (Fig. 2b). Taken together with the pull-down analysis, these results demonstrate the direct interaction between sesamin and Steroleosin B.
Steroleosin B is expressed during seed development and post-germination stages
Simultaneous regulation of Steroleosin B and CYP81Q1 in sesame plants is necessary to achieve interactions with Steroleosin B and sesamin. To investigate the regulatory mechanisms for Steroleosin B expression in sesame plants, we performed reverse transcriptase-polymerase chain reaction (RT-PCR) analyses using total RNA samples from seeds at various stages of development (Supplementary Fig. S2). The resulting data showed that Steroleosin B mRNA is expressed throughout the development of sesame seeds. CYP81Q1 mRNA, which encodes a sesamin synthase, was detected in all stages of seed development5. In addition, Steroleosin B and CYP81Q1 were both expressed during seed germination stages regardless of light conditions, indicating that Steroleosin B expression coincides with that of CYP81Q1 during most of the stages of seed development and germination stages in sesame plants.
Ectopic accumulation of sesamin and Steroleosin B in transgenic Arabidopsis plants results in drastic changes in morphology
We determined whether the functions of sesamin in planta are dependent on Steroleosin B because sesamin interacted with Steroleosin B in vitro. Specifically, we investigated phenotypic changes in plant physiology and morphology in the presence of both sesamin and Steroleosin B. Arabidopsis thaliana plants do not accumulate sesamin but they do contain the precursor pinoresinol and are free from the background effects of endogenous sesamin. In addition, experimental tools for genetic analyses have been well developed in Arabidopsis plants, whereas transformation and gene-knockout techniques are not practical in sesame plants. Moreover, we presumed that external administration of sesamin would not be effective in plant bioassays due to sesamin’s poor water solubility.
We previously showed that the heterologous expression of the sesamin synthase gene CYP81Q1 in transgenic Forsythia koreana cell lines was sufficient for the conversion of endogenous pinoresinol to sesamin (Fig. 3a)13,14,15. Thus, in the present studies using Arabidopsis plants, we induced ectopic accumulation of sesamin by imposing a heterologous expression of the CYP81Q1 gene. Specifically, the coding sequence of CYP81Q1 was inserted downstream of the constitutively active cauliflower mosaic virus 35 S promoter (Pro35S), and the resulting construct was used to transform Arabidopsis plants. After confirming the heterologous expression of CYP81Q1 in the transgenic Pro35S:CYP81Q1 Arabidopsis plants (Fig. 3b), we detected ectopic accumulation of sesamin in the shoots and roots of Pro35S:CYP81Q1, but not wild-type Arabidopsis plants (Fig. 3c,d).
Concurrently, the Steroleosin B gene was inserted downstream of the Pro35S sequence of the pFAST vector16 (Pro35S:Steroleosin B) and introduced into wild-type Arabidopsis plants. The resultant Pro35S:Steroleosin B Arabidopsis plants showed heterologous expression of the Steroleosin B gene (Supplementary Fig. S3a). Our phenotypic analysis demonstrated that Pro35S:CYP81Q1 and Pro35S:Steroleosin B plants showed normal morphology and growth (Fig. 4a,c,d,g,i, Supplementary Fig. S3b). Therefore, ectopic accumulation of sesamin or Steroleosin B individually has no effect on physiology and morphology.
In further experiments, ectopic accumulation of both sesamin and Steroleosin B was achieved in Arabidopsis plants by introducing the Pro35S:Steroleosin B construct into Pro35S:CYP81Q1 Arabidopsis plants. The resulting 28 T1 generation lines of Pro35S:Steroleosin B/Pro35S:CYP81Q1 plants were selected based on their herbicide resistance. Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants of the T1 generation were stunted and formed small leaves(Supplementary Fig. S4a). The six lines of Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants were significantly smaller than the vector control pFAST/Pro35S:CYP81Q1 Arabidopsis plants and showed defective leaf expansion (Supplementary Fig. S4a). The stunted plants died before fertilization or produced low numbers of viable seeds (Supplementary Fig. S4b). The remaining five Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis lines showed moderate defects in leaf expansion and produced seeds that were capable of germination.
To quantitatively and reproducibly compare morphological phenotypes, we established four homozygous T3 generation lines of Pro35S:Steroleosin B/Pro35S:CYP81Q1 and pFAST/Pro35S:CYP81Q1 Arabidopsis plants (Fig. 4a). Initially, we confirmed the heterologous expression of Steroleosin B and CYP81Q1 genes and ectopic accumulation of sesamin in the Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants (Figs 3d, 4b) and then performed quantitative analyses of leaf sizes (Fig. 4c,d). These measurements demonstrated that four-week-old Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants had significantly smaller leaves than the wild-type, Pro35S:CYP81Q1, Pro35S:Steroleosin B, and control pFAST/Pro35S:CYP81Q1 Arabidopsis plants (Fig. 4a,c,d). For comparison of cell number in leaves, the mesophyll palisade cells were counted (Fig. 4e,f). Notably, leaf size reduction was associated with a decreased number of cells in leaves (Fig. 4g). In addition, Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants showed moderately suppressed root elongation (Fig. 4h,i).
In contrast to Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants, single transgenic Pro35S:CYP81Q1 and Pro35S:Steroleosin B plants exhibited normal morphologies (Fig. 4a,c,d and Supplementary Fig. S3b). These data confirm that ectopic accumulations of sesamin and Steroleosin B in Arabidopsis plants suppress leaf growth and root elongation, whereas accumulation of sesamin or Steroleosin B alone had no significant effect on morphology.
Steroleosins are minor integral proteins localized in the membrane of oil body in S. indicum and various other plant species17,18,19. Steroleosins exist as two isoforms, namely Steroleosin A and B, in S. indicum. They have an anchor domain in their N-terminal region that is required for the association with oil body membrane, whereas their C-terminal region has moderate sequence similarity to hydroxysteroid dehydrogenases (HSD) in animals18,20. HSDs in mammals have been shown to maintain steady-state concentrations of various steroid hormones by converting the hydroxy groups into ketones that are crucial for the biological activities of mammalian steroid hormones. No endogenous substrates for Steroleosins have been identified in plants, and thus the biological roles of Steroleosins have not been fully elucidated.
The present in vitro observations of sesamin binding to Steroleosin B (Figs 1 and 2) and consequent measurable growth suppression in transgenic Arabidopsis plants producing both sesamin and Steroleosin B (Fig. 4) offer insight into the biological roles of sesamin and its protein target. Our control plants, Pro35S:CYP81Q1 and Pro35S:Steroleosin B, demonstrated that accumulation of sesamin or Steroleosin B alone caused no phenotypic changes, supportive of no interference between endogenous hydroxysterol dehydrogenases and sesamin or between Steroleosin B and endogenous lignans other than sesamin in transgenic Arabidopsis plants21. Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants showed measurable phenotypic changes indicative of their functional interactions in planta. These observations are quite valuable as no reliable, general methodology exists for detecting interactions between natural small molecules without chemical modification and their binding proteins in planta. Since expression of CYP81Q1 and Steroleosin B genes was driven by the same Pro35S promoter, it is possible that sesamin and Steroleosin B accumulate in the same tissues and cells. Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants provide important clues for understanding the biological significance of sesamin and Steroleosin B.
Our data provide evidence that sesamin plays important roles in phenotypic expression profiles that are indispensable for the survival of plants in severe environments because growth suppression is a common and an important strategy for plants to cope with environmental changes, such as water availability and pathogen attack22,23. In particular, the suppression of leaf growth and root elongation by the combined presence of sesamin and Steroleosin B resembled that of transgenic Arabidopsis plants expressing an RNA interference gene for the Hydroxysterol dehydrogenase 1 (AtHSD1)24. The amino acid sequence of the encoded AtHSD1 protein has a 56% similarity with that of Steroleosin B and the related enzyme activity plays roles in the regulation of leaf and root expansion and in seed germination24. Since AtHSD1 is involved in the brassinosteroid signalling pathway that regulates plant growth and development24, it is possible that sesamin and Steroleosin B potentially affect this endogenous pathway and influence the phenotype of Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants. Collectively, these observations suggest that accumulated sesamin and Steroleosin B interfere with endogenous AtHSD1 activity and suppress the growth of Arabidopsis plants. Although the putative activities of Steroleosin B and AtHSD1 are associated with dehydrogenases24, their endogenous substrates have not been identified. The elucidation of putative effects of sesamin binding to Steroleosin B, and of signalling cascade components that involve sesamin and Steroleosin B, is warranted to better understand the molecular basis of the biological function of Steroleosin B.
Sesamin primarily accumulates in seeds, and to a lesser extent in leaves, in sesame and its content varies among plant tissues and their developmental stages5,25. CYP81Q1 and Steroleosin B genes are co-expressed during seed development and germination (Supplementary Fig. S2). In addition, transgenic Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants showed severe growth defects and produced less siliques compared to Arabidopsis plants harbouring Pro35S:CYP81Q1 only (Supplementary Fig. S4b). From these data, we conclude that Steroleosin B may be required for sesamin to be biologically active and influence plant development; as a result, Steroleosin B and sesamin might be biologically relevant in developing seeds and germinating seedlings. The sesamin content of sesame leaves has been shown to be responsive to changes in light conditions26. Thus, sesamin in sesame plants could also be involved in various aspects of growth control to help cope with changes in environmental conditions.
Our approach using affinity nano-beads together with in planta evaluation of potential impact of sesamin–Steroleosin B interactions on Arabidopsis growth has provided new clues for understanding the biological significance of Steroleosin B-sesamin from the viewpoint of their physical interactions. Previous reports show considerable differences in sesamin content across various sesame cultivars27,28. However, correlation between phenotypic traits and sesamin content have not been previously described. The present data may suggest that Steroleosin B acts cooperatively with sesamin to control growth in sesame plants. Thus, more extensive surveillance of plant traits and measurements of accumulated Steroleosin B and sesamin levels in various sesame cultivars would be of great value to the understanding of their biological roles in sesame plants.
Materials and Methods
Synthesis of sesamin derivative
The experimental details are presented in the Supplementary Information.
Preparation of sesame seedling extracts
In order to obtain extracts, 1 g samples of fresh immature seedlings were harvested and ground into fine powder under liquid nitrogen using a mortar and pestle. Each powdered sample was dissolved in 2.5 ml of extraction buffer containing 100-mM Tris-Cl (pH 8.0), 0.1% β-mercaptoethanol and a cocktail of protease inhibitors (1× Complete EDTA-free, Roche, Japan). Homogenates were then passed twice through nylon mesh and desalted twice using a PD-10 column (GE Healthcare, Japan). Finally, desalted homogenates were subjected to nano-bead-based affinity screening for SBPs.
Preparation of SIB and AIB
First, 2 mg aliquots of magnetic FG-nano-beads (Tamagawa Seiki Co.,Ltd.) were treated with sesamin probe 4 at low and high concentrations of 0.2 and 2 mM, respectively, in the presence of N-hydroxysuccinimide (10 mM) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (10 mM) in dimethylformamide (DMF) at room temperature for 12 h. After washing with DMF, unreacted amino groups on nano-beads were masked using acetic anhydride (200 mM) in DMF for 2 h to obtain SIB. Next, SIB were washed sequentially with DMF, MeOH and phosphate buffered saline (PBS) and subjected to affinity pull-down assays. AIB were prepared by acetylation of amine beads, as described above, without sesamin probe 4 treatment. AIB were used as the control beads.
Affinity purification of SBP using SIB
To purify SBPs, 2 mg samples of SIB were pre-equilibrated with PBS and incubated with the aforementioned sesame seedling extracts in 1 ml aliquots of PBS containing 0.1% NP-40; sesamin was used as a competitor at 2 mM. After washing with PBS, bound proteins were eluted with SDS containing PBS at 90 °C for 5 min, separated using SDS-PAGE and visualised by silver staining. The excised bands were then subjected to in-gel digestion with trypsin. Peptide mass fingerprint analyses were performed using nano liquid chromatography-mass spectrometry (nano LC-MS; Thermo Easy nLC1000 equipped Thermo Orbitrap Elite) and analysed by Mascot search software.
Production of GST-Steroleosin B fusion proteins
Cells from Escherichia coli strain Rosetta2 (DE3; Merck Millipore, Japan) were transformed with pDEST15-GST-Steroleosin B and pre-cultured overnight in 3-ml of LB medium at 37 °C. Flasks containing 50 ml of LB medium supplemented with 50-µg/ml kanamycin were inoculated with 500 µl aliquots of saturated culture. Cells were cultivated until the optical density at 600 nm (OD600) reached 1.0. The cultures were then supplemented with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and cultured for a further 3 h at 23 °C. Next, the cells were harvested by centrifugation at 10,000 × g for 10 min at 4 °C. After removal of supernatant, pellets were stored at −80 °C until purification of GST-fused Steroleosin B, as described elsewhere. Briefly, cell pellets were re-suspended in 15 ml aliquots of ice cold extraction buffer containing 1× PBS, 0.1% β-mercaptoethanol and 1× Complete EDTA-free (Sigma-Aldrich, Japan) and then ultrasonically lysed on ice. Homogenates were finally centrifuged at 10,000 × g for 15 min at 4 °C before being subjected to the pull-down assays using SIB, as described above.
STD-NMR analyses of sesamin and GST-fused Steroleosin B
STD-NMR spectra were acquired using a Bruker AVANCE III HD 800 spectrometer equipped with a 5 mm TCI cryogenic probe and a Z-axis gradient (Bruker Biospin AG, Switzerland). All spectra were measured at 298 K using Wilmad 5 mm NMR tubes. Standard Bruker pulse sequences were employed and residual water signals were suppressed by applying an excitation sculpting sequence.
To prepare the samples for the NMR experiments, sesamin (60 μM) and GST-Steroleosin B or control GST (0.5 μM) were dissolved in 500 μl of PBS that had been prepared with deuterium oxide containing 5% dimethyl sulfoxide-d6 (DMSO-d6). On-resonance irradiation of proteins was performed with a chemical shift of 1 part per million (ppm) and off-resonance irradiation was applied at 30 ppm. Selective irradiation was achieved using Gaussian pulses of 50 ms, resulting in a total saturation time of 3.0 s. Other acquisition parameters were as follows: number of data points, 32 K; spectral width, 9615 Hz; relaxation delay, 5.0 s; number of scans, 512 and receiver gain, 203. Chemical shifts are reported relative to peaks of DMSO-d6, δH observed at 2.50 ppm.
Plant materials and growth conditions
S. indicum plants were grown outdoors in pots unless otherwise indicated, and developing seeds were harvested during designated developmental stages5. Arabidopsis thaliana ecotype Col-0 plants were grown in soil or on plates containing half-strength MS salts, 0.5 g/l MES and 5 g/l sucrose at 22 °C under a 16 h light/8 h dark photocycle. In preparation for harvesting seeds, Arabidopsis plants of various genotypes were arranged side by side and grown on soil until senescence after maturity and then air-dried. In preparation for transforming the Arabidopsis plants, Agrobacterium tumefaciens GV3101 was transformed using the indicated plasmids (as below) using electroporation. The resulting bacteria were used to transform plants using the floral-dip method29 with minor modifications. Transgenic Arabidopsis plants were then selected on plates containing appropriate antibiotics or herbicides. Prior to the determination of sesamin content, the Arabidopsis plants were grown vertically for three weeks on plates.
Construction of plasmids
To clone the coding sequence of the Steroleosin B gene (accession number AAM46847), cDNA was generated using total RNA from developing sesame seeds with oligo dT primers and SuperScript III (ThermoFisher Scientific, Japan) and amplified using the appropriate primer sets. To establish protein production in E. coli, the coding sequence of Steroleosin B was subcloned into the vector pDEST15 (pDEST15-GST-Steroleosin B; ThermoFisher Scientific). Arabidopsis plants were transformed by inserting the coding sequences of CYP81Q1 (accession number AB194714) and Steroleosin B genes downstream of the Pro35S promoter using pBINplus30 and pFAST14 plasmids, respectively.
Analysis of gene expression
Total RNA was isolated from individual tissues of sesame and Arabidopsis plants using RNeasy Plant Mini Kits (Qiagen) and reverse transcribed using SuperScript III (Life Technologies) using oligo dT primers. Aliquots of cDNAs were amplified using ExTaq (Takara, Japan) with the primer sets listed in Supplementary Table S3 and a conventional thermal cycler (Veriti 200; Applied Biosystems). The amplified fragments were then separated on agarose gels and visualized using ethidium bromide. Quantitative real-time PCR analyses were performed using a StepOnePlus real-time PCR system and SYBR Green PCR Master Mix Kits (Life Technologies) with the appropriate primer sets (Supplementary Table S3). CYP81Q1 gene expression levels were quantified relative to ACT2 expression using StepOne Software v2.0 (Life Technologies).
Phenotype analyses of Arabidopsis plants
To quantify leaf length, the leaves at the fourth position of four-week-old Arabidopsis plants were photographed and measured along the longitudinal axis. Comparisons of leaf cell numbers were made by reducing the transparency of the leaf at the fourth position31 and counting the mesophyll palisade cells in the sub-epidermal layers along the longitudinal axis using a light microscope (ECLIPSE; Nikon). To quantify root length, the main roots of two-week-old seedlings were detached and directly measured using a ruler. Statistical analyses were performed using BellCurve for Excel software with the default settings (https://bellcurve.jp/ex/). One-way ANOVA was performed, followed by Dunnett’s test. P < 0.05 was considered statistically significant.
Measurement of sesamin content in Arabidopsis plants
Arabidopsis plants were freeze-dried using a FDU-2110 device (EYELA), extracted twice in 50% methanol at 65 °C for 1 h and then centrifuged at 20,000 × g for 3 min. The resulting supernatants were concentrated under vacuum and filtered using a Millex-LH column (Millipore). To determine the sesamin content, filtrates were applied to an ACQUITY UPLC system (Waters Corp.), as described previously with several modifications32. Briefly, 3-μl aliquots of filtrates were added to ACQUITY UPLC HSS C18 Columns (2.1 × 150 mm, 1.8 μm) at 40 °C and analysed with a Waters 470 Scanning Fluorescence Detector (Waters Corp.) with excitation and emission wavelengths set at 280 and 340 nm, respectively. Mobile phases comprised 5% (v/v) acetonitrile in ultra pure water containing 0.05% (v/v) acetic acid (solvent A) and 95% (v/v) acetonitrile in ultra pure water with 0.05% (v/v) acetic acid (solvent B). The gradient elution programme was as follows: 60–90% B for 0–6 min, 90–95% B for 6–6.5 min, 95–90% B for 6.5–8 min, 90–60% B for 8–12 min and 60% B for 12–13 min at a flow rate of 0.3 ml/min. Chromatographic peaks were identified in comparison with the retention times of authentic sesamin and quantified from the peak areas using an external standard method. The resulting chromatograms were then analysed using Waters Empower 2 software.
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We thank Michio Sugahara (Kobe University), Erika Matsumoto and Keiko Shimamoto (Suntory Foundation for Life Sciences) for the technical support and fruitful discussions. MS is a lead of JST, ERATO Suematsu Gas Biology Project until March 2015 which contributed to a platform for affinity-beads screening method.
Eiichiro Ono is an employee of Suntory Global Innovation Center, Ltd. Suntory Foundation for Life Sciences is a non-profit organization. The corresponding authors had full access to all the data in the study and had final responsibility for the decision to submit for publication.
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