Figure 3 | Scientific Reports

Figure 3

From: Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes

Figure 3

Gene expression profile of Fgf8b and PPARy agonist treated white adipocytes. White adipocytes were treated with Fgf8b (125 ng/ml) and/or rosiglitazone (20 µM) during differentiation. (A,B) Transcript abundance of genes indicative for brown adipocytes, brite markers, adipogenesis, and FGF signalling (abbreviations below), n = 3–6. Fgf8b treatment leads to significantly different abundance of all transcripts analysed except Tbx1, Slc21a1 and Fgfrs 3 and 4 both in the absence and in the presence of rosiglitazone and except Fgf21 in the presence of rosiglitazone (Holm-Sidak multiple testing). (C) Representative Western Blot detecting terminal adipogenic differentiation markers HSL and perilipin and mitochondrial marker COX4. (D) Quantified, normalized band intensity of proteins detected in (C), n = 3, bars represent mean values ± SD. Stars indicate a significantly different abundance of this transcript as compared to the control. Ucp1, uncoupling protein 1; Cidea, cell death-inducing DNA fragmentation factor alpha-like effector A; Pgc1a, peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; Cs, citrate synthase; Prdm16, PR domain containing 16; Tmem26, Transmembrane protein 26; Tbx1, T-box transcription factor 1; Cd137, cluster of differentiation 137; Hoxc9, homeobox C9; Shox2, Short stature homeobox 2; Slc27a1, Long-chain fatty acid transport protein 1; Pparg, peroxisome proliferator-activated receptor gamma; Fabp4, fatty acid binding protein 4; Hsl, hormone-sensitive lipase; Fasn, fatty Acid Synthase; Pref-1, preadipocyte secreted factor-1; Fgfr, fibroblast growth factor receptor; Klb, klotho beta; Fgf21, fibroblast growth factor 21; HSL, hormone sensitive lipase; PLIN, perilipin; COX4, cytochrome C oxidase subunit 4.

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