mDia1 co-immunoprecipitates differentiation markers MyoD, active β-Catenin and pAkt2 Ser474 along with Phb2 during differentiation. C2C12 cells cultured under growth conditions (GM) or differentiated for 72 (D72) hours were lysed and subjected to IP with anti-mDia1 antibody, and analysed by western blotting using respective antibodies. (a) Co-IP of mDia1 with Akt2 and pAkt2 Ser474 in MT. IP samples were loaded on different gels, cut and processed in parallel for detection of mDia1, Akt2 and pAkt2 Ser474. (b) Co-IP of MyoD, Phb2 and Akt2 by mDia1 in MT. (c) Co-IP of active β-Catenin and Phb2 by mDia1 in MT. (d) IP of Phb1, Akt2 and Phb2 by mDia1 in MT. Act β-Cat- Active β-Catenin. For the blots shown in (b–d) the IP samples for MB and MT were run on a single gel, the blot was cut and processed in parallel for detection using the respective antibodies under the same conditions. Uncropped version of the blots represented in (b–d) are shown in Supplementary Fig. S6.