Figure 1 | Scientific Reports

Figure 1

From: Surface modification of gold nanoparticles with neuron-targeted exosome for enhanced blood–brain barrier penetration

Figure 1

Schematic representation of gold nanoparticle surface functionalization through exosome coating. In this approach, exosome-producing cells were transiently transfected with a pcDNA GNSTM-3-RVG-10-Lamp2b-HA vector to express Lamp2b fusion protein fused in frame with the neuron-specific rabies viral glycoprotein (RVG) and glycosylation-stabilized (GNSTM) peptides, allowing the engineered cells to produce and secret exosomes with the RVG and GNSTM peptides on their surface as previously described by Hung, M. E., & Leonard, J. N., 201513. These engineered exosomes were collected then coated onto the surfaces of synthetic nanoparticles using the mechanical method or extrusion. Western blot of exosomes produced from transfected or non-transfected cells and probed with anti-HA antibody against HA tag present in Lamp2b fusion protein are also shown in the figure.

Back to article page