Figure 5 | Scientific Reports

Figure 5

From: Stress-induced tunneling nanotubes support treatment adaptation in prostate cancer

Figure 5

Stress-associated proteins facilitate TNT formation. LNCaP (A) and PC3 (B) cells were transfected with siRNAs followed with CSS or SS treatment and number of TNTs were quantified. *p < 0.001 compared to siScr. (C) PC3 cells were first transfected with siYB-1 and then with CLU-expressing plasmid followed with SS treatment for 24 hours. *p < 0.001 compared to mock. (D) PC3 cells were first transfected with siCLU and then with YB-1-expressing plasmid followed with SS treatment for 24 hours. *p < 0.001 compared to mock. (E) PC3 cells transfected with siAKT or treated with LY294002 were challenged with SS for 24 hours and then processed for quantification of TNT formation. *p < 0.001 compared to ctrl. (F) PC3 cells transfected with vectors for CLU, YB-1 or mock were treated with SS +/− LY294002. *p < 0.001 compared to mock. (G) LNCaP cells transfected with siAKT or treated with LY294002 were exposed to CSS or ENZA for 24 hours and then processed for TNT quantification. *p < 0.001 compared to ctrl. (H) PC3 cells were transfected with CLU, YB-1 or mock vector; 24 hours later cells were transfected again with siScr or siEsp8, followed with another 24 hours of SS treatment. TNTs formation was quantified. *p < 0.05 compared to SS condiction in the siScr group. (I) PC3 cells transfected with CLU, YB-1 or mock vector were challenged with the Eps8 inhibitor mithramycin A in the presence os SS for 24 hours. TNTs formation was quantified. *p < 0.001 compared to ctrl. All data is presented as average +/− SD from 3 independent experiments.

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