Table 1 Overview of the different RNA preparation kits and conditions analyzed in this study.

From: Systematic analysis of TruSeq, SMARTer and SMARTer Ultra-Low RNA-seq kits for standard, low and ultra-low quantity samples

Kit Manufacturer RNA type Input (ng) Stranded Acronym PC
Illumina technology
mRNA TruSeq Illumina mRNA 1000 no mRNATruseq_1 ug 13
Stranded mRNA TruSeq Illumina mRNA 1000 yes ssmRNATruseq_1 ug 13
Stranded total RNA TruSeq Illumina total RNA 1000 yes ssTotRNATruseq_1 ug 14
Stranded mRNA TruSeq Illumina mRNA 100 yes ssmRNATruseq_100 ng 15
Stranded total RNA TruSeq Illumina total RNA 100 yes ssTotRNATruseq_100 ng 15
SMARTer technology
RiboGone Stranded SMARTer Takara Bio total RNA 100 yes RiboG_ssTotSmarter_100 ng 18
RiboZero Gold stranded SMARTer Illumina + Takara Bio total RNA 100 yes RiboZ_ssTotSmarter_100 ng 18
RiboZero Gold stranded SMARTer Illumina + Takara Bio total RNA 10 yes RiboZ_ssTotSmarter_10 ng 18
SMARTer Ultra-Low technology
SMARTer UL + Nextera Takara Bio + Illumina mRNA 1 (0.75) no mRNASmarterUL_XT1 ng_750 pg 11/12
SMARTer UL + Nextera Takara Bio + Illumina mRNA 1 (0.13) no mRNASmarterUL_XT1 ng_130 pg 11/12
  1. PC: number of PCR cycles done for each application. For the Ultra-Low SMARTer kits, the first digit is the number of cycles for the LD-PCR (first step), and the second digit is the number of cycles for the PCR (second step).