Table 1 qPCR probes, oligonucleotides and antibodies used in this study.

From: PA-X antagonises MAVS-dependent accumulation of early type I interferon messenger RNAs during influenza A virus infection

qRT-PCR
Taqman probes (Thermo Fisher Scientific) Assay Probe ID Oligos (Sigma Aldrich) Sequence Reference
Gene Gene
Gapdh Cat. No. 4352932E M F: 5′-CTTCTAACCGAGGTCGAAACGTA Shin et al. 2013, Virology Journal 10:303
Ifnb1 Mm00439546_s1 R: 5′-GGTGACAGGATTGGTCTTGTCTTTA
Ifna4 Mm00833969_s1 Gapdh F: 5′-CATGGCCTTCCGTGTTCCTA Tsujita et al. 2006, PNAS 103:11946–11951
Ifna5 Mm00833976_s1 R: 5′-CCTGCTTCACCACCTTCTTGAT
Ifna6 Mm01703458_s1    
Ifi44 Mm00505670_m1    
Ifit1 Mm00515153_m1    
Isg20 Mm00469585_m1    
Il28a Mm04204158_gh    
Ifng Mm01168134_m1    
Oligos to generate probes for Northern blots
Gene Sequence Template for PCR Reference  
Ifnb1 F: 5′-ATGAACAACAGGTGGATCC pGEM-IFN-β Schulz et al.49, Cell Host Microbe 7:354–361  
R: 5′-GGCATCAACTGACAGGTCTT   
Actb F: 5′-GACTTTGTACATTGTTTTG cDNA from PR8 WT-infected mouse lung This study  
R: 5′-TGCACTTTTATTGGTCTCA  
Oligos for ePAT
Name Sequence Reference   
PAT-anchor primer 5′-GCGAGCTCCGCGGCCGCGTTTTTTTTTTTT Janicke et al.19, RNA 18:1289-1295   
TVN-PAT primer 5′-GCGAGCTCCGCGGCCGCGTTTTTTTTTTTTVN Janicke et al.19, RNA 18:1289-1295   
mIfnb1 ePAT primer 5′-ACCTGTCAGTTGATGCCTCA This study   
Antibodies used for Western blot
Antigen Supplier Host Cat. number Dilution
PA GeneTex Rabbit pAb GTX125932 1:1000
MAVS ENZO Life Sciences Rabbit pAb ALX-210-929-C100 1:500
RIG-I AdipoGen Mouse mAb AG-20B-0009 1:1000
p-IRF3 Cell Signaling Technology Rabbit mAb 4947S 1:1000
β-actin Sigma Mouse mAb (HRP-coupled) A3854 1:100,000