Figure 3 | Scientific Reports

Figure 3

From: PA-X antagonises MAVS-dependent accumulation of early type I interferon messenger RNAs during influenza A virus infection

Figure 3

PA-X does not affect the expression of type I IFN protein or ISGs in the lung. Wild-type mice were infected with 50,000 pfu of PR8 WT, PR8 FS or mock infected via the intranasal route and sacrificed 48 h.p.i. (A) Quantification of IFN-β and IFN-α protein in the BAL fluid by ELISA. Data are from 3–7 (mock) and 7–12 (infected) mice per group ± s.e.m. and are pooled from four independent experiments. (B) Levels of Ifna5 and Ifna6 mRNA in the lung were measured by qRT-PCR. Data are from 4 (mock) and 14 (infected) mice per group and are represented as expression relative to the housekeeping gene Gapdh ± s.e.m., pooled from 4 independent experiments. (C) Ifi44, Ifit1 and Isg20 mRNA levels in the lungs were quantified using qRT-PCR. Data are from 4–28 (mock) and 11–49 (infected) mice per group ± s.e.m. and are pooled from 11 (Ifi44) or three (Isg20 and Ifit1) independent experiments. (D) Quantification of IP-10 protein in the BAL fluid by ELISA. Data are from 7 (mock) and 11 (infected) mice per group ± s.e.m. and are pooled from four independent experiments. Expression of (E) Il28a and (F) Ifng in the lungs were quantified by qRT-PCR. Data are from 14 (mock) and 30–31 (infected) mice per group ± s.e.m. and are pooled from 8 independent experiments. *P ≤ 0.05, ns not significant; unpaired Student’s t-test.

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