Figure 1 | Scientific Reports

Figure 1

From: PA-X antagonises MAVS-dependent accumulation of early type I interferon messenger RNAs during influenza A virus infection

Figure 1

Generation of a PA-X-deficient PR8 virus. (A) Schematic illustration of the two viruses used in this study: ‘PR8 WT’ and ‘PR8 FS’ which has greatly reduced translation of PA-X due to mutations in the frameshift motif. (B) HEK293T cells, pre-treated overnight with 100 U/ml IFN-A/D to increase expression of RIG-I to detectable levels, were infected with PR8 WT, PR8 FS or Sendai virus (SeV) at increasing MOIs (0.1, 1, 10). Cells were harvested for immunoblotting 24 h.p.i. using the indicated antibodies. Full-length blots are shown in Fig. S2.

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