Figure 3 | Scientific Reports

Figure 3

From: Expansion of different subpopulations of CD26−/low T cells in allergic and non-allergic asthmatics

Figure 3

CD4 lymphocytes with a highly differentiated memory phenotype are expanded in non-allergic asthma. (a) CD4 lymphocytes were identified by flow cytometry (left and middle dot plots). Then CD127 and CD26 were used to identify five subsets within the CD4 lymphocyte compartment (right dot plot). (b) Percentage of CD4CD26+ or CD4CD126+ cells from healthy controls (HC; N = 32), rhinitis (R; N = 44), allergic asthma (AA; N = 100) and non-allergic asthma (NAA; N = 92). (c) Percentage (median ± IQR1-3) of CD26CD127, CD26intCD127+, and CD26highCD127+ cells amongst CD4 lymphocytes in HC, R, AA, and NAA. (d) Composition of CD4 lymphocyte subsets based on CD3 (T), CD8 (Tc), CD19 (B), and CD56 (NK, NKT) antigens (Data from 3 representative donors). (e) Phenotypic analysis of CD4 subsets based on CD45RA, CCR7, CD127, CD28, and CD27 markers (data from 3 representative donors). Statistically significant differences between groups are indicated (Kruskal-Wallis test: P < 0.05). In order to make the figure more understandable, sections (c,d and e) show only those CD4 subsets gated in (a) (right dot plot) where differences between groups were observed (CD26CD127, CD26intCD127+, and CD26highCD127+ cells).

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