Figure 6 | Scientific Reports

Figure 6

From: Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging

Figure 6

Detection of 5mC-foci with high-resolution Raman imaging of HCT116 cells and mESCs. (a) Anti-5mC immunofluorescence staining of HCT116 WT and DNMT1−/− cells. (b) Heat maps of the Raman images obtained from HCT116 WT and DNMT1−/− cells using the sum intensities of 1257 cm−1, 1379 cm−1 and 1579 cm−1. (c) Average spectra from high-intensity regions and low-intensity regions of the heat maps from panel (b). (d,h) Statistical analysis of the Raman images from panels b and f. Two-tailed t-test, n = 3. (e) Anti-5mC immunofluorescence staining of mESC control and 2i mESCs. (f) Heat maps of the Raman images from mESC control and 2i mESCs using the sum intensities described in panel b. (g) Average spectra from high-intensity regions and low-intensity regions of the heat maps from panel f. Scale bars in all images equal 5 µm.

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