Figure 5 | Scientific Reports

Figure 5

From: Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging

Figure 5

Comparison of Raman spectra from mouse pluripotent stem cells cultured in either control or 2i medium with human colon cancer WT and DNMT−/− cells. (a) 3D score plot of Raman spectra from control mESCs, 2i mESCs as well as human colon cancer WT and DNMT1−/− cells showing PC 2, PC 4 and PC 5. There is a grouping tendency between control mESCs and WT cells (blue shades), as well as 2i mESCs, DNMT1−/− cells (green shades). (b) The grouping tendency is visualized by a box plot of PC 5 score values. It shows significantly lower scores for control mESCs and WT cells when compared with 2i mESC and DNMT1−/− cells. One-way ANOVA, n = 30.

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