Figure 3 | Scientific Reports

Figure 3

From: Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging

Figure 3

Investigation of global DNA methylation changes. (a) 5mC expression in mESCs cultured under control conditions or in 2i medium. 2i medium-adapted cells show a significantly lower DNA methylation. Two-tailed t-test, n = 3. (b) Immunofluorescence staining of 5mC. Scale bars equal 20 µm. (c + d) Quantitative fluorescence intensity analysis of 5mC-stained mESCs. 2i medium-cultured mESCs show a significant lower mean intensity and less 5mC-foci/cell (indicated by white arrows in b) when compared with cells cultured in the presence of serum (control). Two-tailed t-test, n = 3.

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