Figure 1 | Scientific Reports

Figure 1

From: Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging

Figure 1

Investigation of global DNA methylation in HCT116 WT and DNMT1−/− cells. (a) Immunofluorescence staining of 5-methylcytosine (5mC) in WT and DNMT1−/− cells. WT cells show various dense 5mC-foci within the nucleus indicated by white arrows. Scale bars equal 20 µm. (b,c) Semi-quantitative fluorescence intensity analysis of 5mC-stained cells. WT cells show significant more 5mC-foci/cell than DNMT1−/− cells. Two-tailed t-test, n = 3. (d) Global 5mC level in WT cells are significantly higher than in DNMT1−/− cells. Two-tailed t-test, n = 3.

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