Figure 3 | Scientific Reports

Figure 3

From: Ligustilide, a major bioactive component of Angelica sinensis, promotes bone formation via the GPR30/EGFR pathway

Figure 3

LIG attenuates H2O2-induced apoptosis in MC3T3-E1 cells. (a,b) Cell viability was determined by MTT reduction in MC3T3-E1 cells in the presence of H2O2. Error bars indicate s.e.m. (n = 6). (c) Representative immunoreactive bands for Bcl-2 and Bax in MC3T3-E1 cells in the presence of H2O2. Quantification of immunoreactive bands for Bcl-2 (d) and Bax (e) relative to GAPDH. Error bars indicate s.e.m. (n = 3). (f) and (g) Cell viability was determined by MTT reduction in MC3T3-E1 cells with LIG in the presence or absence of H2O2. Error bars indicate s.e.m. (n = 18). (h) and (i) Flow cytometric quantification of apoptosis. Error bars indicate s.e.m. (n = 3). ###P ≤ 0.001 vs. control group. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. model group.

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