High miR-130b expression was correlated with poor overall survival in NSCLC clinical specimens. (A–C) TCGA database analysis of miR-130b, miR-301a, and miR-301b in patients with adenocarcinoma. Overall survival analysis in patients with high (copy number: 1) and low (copy number: −1) miR-130 family expression was analysed by Kaplan-Meier analysis with log-rank tests. The number of patients analysed is indicated in parentheses. miR-130b expression levels were measured by real-time qPCR and were compared among normal and tumor tissues (D) and tumor stages (E) in NSCLC clinical specimens, tumor subtypes in specimens with adenocarcinoma or squamous cell carcinoma (F), status of epidermal growth factor receptor gene mutation in specimens with adenocarcinoma (G). Relative miR-130b expression normalized to U6 snRNA is shown. Data are means ± standard deviations. **p < 0.01 and ***p < 0.001 for t-tests (D) and one-way analysis of variance (post-hoc Bonferroni multiple comparison tests) (E–G).