ZIKV infection during pregnancy via in utero impairs spinal cord development and function. ZIKV (500 PFU) and mock infection were conducted via in utero at E13.5. (A) fluorescent Nissl staining and quantification of positive cells in the anterior horn of the grey matter (B, n = 3–5 per group, >500 cells were counted) at P30. (C) NeuN staining and quantification of positive cells (D, n = 3–5 per group, >500 cells were counted) at P30. (E) Images of mouse limbs, weights (F), and forepaw grip analyses (G, n = 3–5 per group) at P30. (H) HE staining of hind limb muscle at P30. (I) Images of footprints and analysis at P30 (J, n = 3–5 per group). **P < 0.01, ***P < 0.001, calculated by Student’s t-test. Graphs indicate mean ± SD, Scale bar: A, C, left panel, 200 µm; enlarged images, 50 µm. H, 20 µm.