Figure 3 | Scientific Reports

Figure 3

From: In utero infection of Zika virus leads to abnormal central nervous system development in mice

Figure 3

ZIKV leads to postnatal brain reduction and impairs young adult neurogenesis. ZIKV (500 PFU) and mock infection were conducted via in utero at E13.5. (A) NeuN staining and thickness calculation (B, n = 3–5 per group) of brain sections at P30. (C) Images show NeuN staining of five layers of the cortex and thickness analyses (D, n = 3–5 per group). (E) Images show the hippocampus, the area calculation (F) and the CA1 image (G) and thickness (H, n = 3–5 per group) at P30. (I) Ki67 staining (J) and quantification of positive cells in the SVZ (J) and Ki67 staining (K) and quantification of positive cells in the DG (L, n = 3–5 per group). >1000 cells were counted in SVZ; >150 cells were counted in DG at P30. (M) DCX staining and quantification of DCX-positive cells (N, n = 3–5 per group, >500 cells were counted) at P30 in hippocampus DG. (0,P) Spontaneous Y-maze test at P30 (n = 3–5 per group). *P < 0.05, **P < 0.01, calculated by Student’s t-test. NS, not significant. Graphs indicate mean ± SD. Scale bar: A, 1 mm; C, 100 µm; E, 500 µm; G, I, K, and M, 20 µm.

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