ZIKV infection during pregnancy induces mouse brain development disorder after birth. ZIKV (500 PFU) and mock infection were conducted via in utero at E13.5. (A) E17.5 embryos morphology post- ZIKV and mock infection. (B) Brain images at E17.5. (C) brain weights at E17.5 (n = 3 per group). (D,E) ZIKV detection in the embryo cortex by immunostaining (D) and qPCR (E, n = 3–4 per group). Scale bar: left panels, 50 µm; enlarged images in the right panels, 10 µm. (F) Pups at P3, P9 and P30 and (G) changes in their body weights post-ZIKV infection (n = 5, ZIKV 500 PFU) at different postnatal time points. (H) Brain image and (I) brain weight at P9 after ZIKV infection (500 PFU, n = 5–6 per group). (J) Brain images and (K) brain weight at P30 after ZIKV infection (500 PFU, n = 6 per group). *P < 0.05, **P < 0.01, ***P < 0.001, calculated by Student’s t-test. NS, not significant. Graphs indicate mean ± SD.