Effect of GANT61 on cell proliferation and apoptosis of breast cancer cells. Cell viability assays were conducted using CCK-8, to observe the effect of GANT61 on cell proliferation in MDA-MB-231 and MCF-7 in both (a). Dose dependent (5, 10, 15, 20 µM) and (b). Time dependent manner (24, 48, 72, 96hrs). IC50 of GANT61 was determined to be 10 µM. MDA-MB-231 and MCF-7 cells were treated with 10uM GANT61 and the control medium containing DMSO (Unpaired t test, ***, ###p < 0.0001). Cell apoptosis was assessed using Annexin V-Cy3 kit and readings were taken after 48hrs of exposure at different concentrations (5, 10, 15, 20 µM) for (c). MDA-MB-231 and (d). MCF-7. Histograms showing quantitative assessment of apoptosis in c and d. Scatter plots and peaks in first panel of c and d represent untreated, unlabeled control, second panel untreated labeled control, and other panels illustrate different doses of GANT61. All results are representative of three independent experiments.